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Research Article


Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia

Kholik Kholik, Akhmad Sukri, Katty Hendriana Priscilia Riwu, Shendy Canadya Kurniawan, Aswin Rafif Khairullah.


Abstract
Background:
Bacterial identification can be done using various testing techniques. Molecular techniques are often used to research dangerous diseases, an approach using genetic information on the pathogenic agent. The enterohemorrhagic invasive species Escherichia coli 0157:H7 was identified from the feces of working horses on the island of Sumbawa. Another advance in molecular technology is genome amplification with qPCR which is the gold standard for detecting E. coli.
Aim:
This study aims to detect and identify the invasive species Escherichia coli 0157:H7 using the gene encoding chuA with the qPCR method sourced from horse feces.
Methods:
Fresh fecal samples from horses on Sumbawa Island were isolated and identified, then continued with molecular examination using the gene encoding chuA using the qPCR method.
Results:
qPCR testing in this study showed that 6 sample isolates that were positive for Escherichia coli 0157:H7 were detected for the presence of the chuA gene, which is a gene coding for an invasive species of E. coli bacteria. The highest to lowest Cq values and Tm from the qPCR results of the sample isolates were 15.98 (4KJ), 14.90 (19KG), 14.6 (3KJ), 13.77 (20KG), 12.56 (5KGB), 12.20 (6KJ). Tm values are 86.7 (4KJ), 86.69 (3KJ), 86.56 (5KGB), 85.88 (20KGB), 85.81 (19KG), 85.74 (6KJ).
Conclusion:
Validation, standardization of the development, and modification of qPCR technology must be carried out to harmonize testing throughout to avoid wrong interpretation of the test results so that the determination of actions to eradicate and control diseases originating from animals in the field does not occur.

Key words: Escherichia coli 0157:H7, chuA, qPCR, Horse, Invasive


 
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How to Cite this Article
Pubmed Style

Kholik K, Sukri A, Riwu KHP, Kurniawan SC, Khairullah AR. Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia. Open Vet J. 2024; 14(4): 1051-1058. doi:10.5455/OVJ.2024.v14.i4.12

Web Style

Kholik K, Sukri A, Riwu KHP, Kurniawan SC, Khairullah AR. Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia. https://www.openveterinaryjournal.com/?mno=186481 [Access: May 02, 2024]. doi:10.5455/OVJ.2024.v14.i4.12

AMA (American Medical Association) Style

Kholik K, Sukri A, Riwu KHP, Kurniawan SC, Khairullah AR. Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia. Open Vet J. 2024; 14(4): 1051-1058. doi:10.5455/OVJ.2024.v14.i4.12


Vancouver/ICMJE Style

Kholik K, Sukri A, Riwu KHP, Kurniawan SC, Khairullah AR. Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia. Open Vet J. (2024), [cited May 02, 2024]; 14(4): 1051-1058. doi:10.5455/OVJ.2024.v14.i4.12


Harvard Style

Kholik, K., Sukri, . A., Riwu, . K. H. P., Kurniawan, . S. C. & Khairullah, . A. R. (2024) Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia. Open Vet J, 14 (4), 1051-1058. doi:10.5455/OVJ.2024.v14.i4.12


Turabian Style

Kholik, Kholik, Akhmad Sukri, Katty Hendriana Priscilia Riwu, Shendy Canadya Kurniawan, and Aswin Rafif Khairullah. 2024. Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia. Open Veterinary Journal, 14 (4), 1051-1058. doi:10.5455/OVJ.2024.v14.i4.12


Chicago Style

Kholik, Kholik, Akhmad Sukri, Katty Hendriana Priscilia Riwu, Shendy Canadya Kurniawan, and Aswin Rafif Khairullah. "Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia." Open Veterinary Journal 14 (2024), 1051-1058. doi:10.5455/OVJ.2024.v14.i4.12


MLA (The Modern Language Association) Style

Kholik, Kholik, Akhmad Sukri, Katty Hendriana Priscilia Riwu, Shendy Canadya Kurniawan, and Aswin Rafif Khairullah. "Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia." Open Veterinary Journal 14.4 (2024), 1051-1058. Print. doi:10.5455/OVJ.2024.v14.i4.12


APA (American Psychological Association) Style

Kholik, K., Sukri, . A., Riwu, . K. H. P., Kurniawan, . S. C. & Khairullah, . A. R. (2024) Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia. Open Veterinary Journal, 14 (4), 1051-1058. doi:10.5455/OVJ.2024.v14.i4.12


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