E-ISSN 2218-6050 | ISSN 2226-4485
 

Research Article


Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle

Naglaa Ebrahim Aly, Mohamed Hassan Atwa, Amany Mohamed Abbas, Diana Mohamed Abulmagd, Zeinab Taha Salem, Taradi Abdelfattah Sayed.


Abstract
Background:
The target of vaccination is to encourage a strong, covering and long-lasting immune response against antigens. For achieving these objectives; effective adjuvant and new vaccine strategies are demanded to make the vaccine sufficiently immunogenic to instigate a powerful immune response
Aim:
This study was completed for elaboration and evaluation of freeze-dried combined vaccine against both Rift Valley fever (RVF) and Bovine Ephemeral Fever (BEF) viruses using different stabilizers.
Methods:
Three formulae were prepared from such vaccine including: formula (1): stabilized with a mixture of 5% Lactalbumin Hydrolysate and 2.5% sucrose, formula (2): stabilized with a mixture of 50% the previous stabilizer and 50% of 1% Carbopol and formula (3): stabilized with 1% Carbopol solution. Samples of the three vaccine formulae were reconstituted on the time of experimental animal vaccination using saponin diluent which acts as an adjuvant for both RVFv and BEFv and as inactivator BEF virus. The ratio between both viruses in all vaccine formulae was 1:1.
Results:
All vaccine batches were proved to be free of any foreign contaminants and unharmed for experimentally vaccinated animals. Each of three groups of calves was vaccinated S/C with 2ml of a reconstituted vaccine formula and their immune response was evaluated using serum neutralization test (SNT). The gained results revealed that the prepared combined freeze-dried vaccine with Carbopol elicited better humoral immune response than the other two vaccine formulae.
Conclusion:
It could be recommended to use Carbopol as a stabilizer for preparation of the aimed vaccine.

Key words: Rift Valley fever, Bovine ephemeral fever, Neutralization test, Cytopathic effect


 
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How to Cite this Article
Pubmed Style

Aly NE, Atwa MH, Abbas AM, Abulmagd DM, Salem ZT, Sayed TA. Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle. Open Vet J. 2023; 13(7): 826-833. doi:10.5455/OVJ.2023.v13.i7.3


Web Style

Aly NE, Atwa MH, Abbas AM, Abulmagd DM, Salem ZT, Sayed TA. Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle. https://www.openveterinaryjournal.com/?mno=138162 [Access: February 13, 2024]. doi:10.5455/OVJ.2023.v13.i7.3


AMA (American Medical Association) Style

Aly NE, Atwa MH, Abbas AM, Abulmagd DM, Salem ZT, Sayed TA. Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle. Open Vet J. 2023; 13(7): 826-833. doi:10.5455/OVJ.2023.v13.i7.3



Vancouver/ICMJE Style

Aly NE, Atwa MH, Abbas AM, Abulmagd DM, Salem ZT, Sayed TA. Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle. Open Vet J. (2023), [cited February 13, 2024]; 13(7): 826-833. doi:10.5455/OVJ.2023.v13.i7.3



Harvard Style

Aly, N. E., Atwa, . M. H., Abbas, . A. M., Abulmagd, . D. M., Salem, . Z. T. & Sayed, . T. A. (2023) Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle. Open Vet J, 13 (7), 826-833. doi:10.5455/OVJ.2023.v13.i7.3



Turabian Style

Aly, Naglaa Ebrahim, Mohamed Hassan Atwa, Amany Mohamed Abbas, Diana Mohamed Abulmagd, Zeinab Taha Salem, and Taradi Abdelfattah Sayed. 2023. Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle. Open Veterinary Journal, 13 (7), 826-833. doi:10.5455/OVJ.2023.v13.i7.3



Chicago Style

Aly, Naglaa Ebrahim, Mohamed Hassan Atwa, Amany Mohamed Abbas, Diana Mohamed Abulmagd, Zeinab Taha Salem, and Taradi Abdelfattah Sayed. "Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle." Open Veterinary Journal 13 (2023), 826-833. doi:10.5455/OVJ.2023.v13.i7.3



MLA (The Modern Language Association) Style

Aly, Naglaa Ebrahim, Mohamed Hassan Atwa, Amany Mohamed Abbas, Diana Mohamed Abulmagd, Zeinab Taha Salem, and Taradi Abdelfattah Sayed. "Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle." Open Veterinary Journal 13.7 (2023), 826-833. Print. doi:10.5455/OVJ.2023.v13.i7.3



APA (American Psychological Association) Style

Aly, N. E., Atwa, . M. H., Abbas, . A. M., Abulmagd, . D. M., Salem, . Z. T. & Sayed, . T. A. (2023) Immunogenicity of a freeze-dried combined vaccine against Rift Valley fever and bovine ephemeral fever in cattle. Open Veterinary Journal, 13 (7), 826-833. doi:10.5455/OVJ.2023.v13.i7.3