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Research Article


Escherichia coli and Salmonella enterica isolated from Egyptian dairy cattle herds: The prevalence and molecular characteristics

Mahmoud S.A. Zaki, Amr M.M. Abd-El-All, Amira S.A. Attia, Hesham Dahshan, Manal A. Al-Ashery, Ayman Megahed.


Abstract
Background:
The pathogens E. coli and S. enterica that caused substantial health problems and financial losses were believed to have originated primarily from Egypt's dairy farms.
Aim:
The purpose of this study was to ascertain the occurrence of E. coli and S. enterica in three large dairy farms located in the Egyptian governorate of Sharkia. Furthermore, biochemical and serological characteristics of the isolated isolates were described. Further analysis revealed that several E. coli serovars had the genes stx1, stx2, eaeA, and hylA, while invA, stn, and hilA genes were found in several S. enterica serotypes using a multi-plex PCR.
Methods:
A total of 540 samples of fresh raw cow milk, water, feedstuffs, feces, (108 each), as well as swabs from feeders, milker hands and cattle crushes (36 each ), were gathered and analyzed.
Results:
The recovery of E. coli from various sampling sources was shown to have an overall prevalence of 62.2% (336/540) in the results. Fecal samples had isolated S. enterica, with a frequency of 0.74% (4/540). The existence of various groups of serovars, such as O26, O44, O55, O78 and O111 for E. coli and S. enteritidis, S. typhimurium and S. inganda for S. enterica was revealed by serological identification of the two species. However, it was discovered that a number of E. coli serovars had much higher percentages of the eaeA and hylA genes as well as shiga-toxin types 1 and 2 (stx1 and stx2). The presence of the invA gene, a diagnostic marker for S. enterica was 100% across all serovars. Salmonella enteritidis possessed both the enterotoxin gene (stn) and the hyper-invasive locus gene (hilA). Salmonella typhimurium had the hilA gene, whereas Sal. inganda had the stn gene.
Conclusion:
E. coli and S. enterica recovered in this study have significant genetic risk factors for high pathogenicity and virulence, posing a real threat to dairy population productivity and health, which could spread to the general public through milk.

Key words: Dairy herds, E. coli, S. enterica, Multiplex PCR, Shiga toxins


 
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How to Cite this Article
Pubmed Style

Zaki MS, Abd-El-All AM, Attia AS, Dahshan H, Al-Ashery MA, Megahed A, . Escherichia coli and Salmonella enterica isolated from Egyptian dairy cattle herds: The prevalence and molecular characteristics. Open Vet J. 2024; 14((1) (Zagazig Veterinary Conference)): 214-224. doi:10.5455/OVJ.2024.v14.i1.19


Web Style

Zaki MS, Abd-El-All AM, Attia AS, Dahshan H, Al-Ashery MA, Megahed A, . Escherichia coli and Salmonella enterica isolated from Egyptian dairy cattle herds: The prevalence and molecular characteristics. https://www.openveterinaryjournal.com/?mno=175952 [Access: February 12, 2024]. doi:10.5455/OVJ.2024.v14.i1.19


AMA (American Medical Association) Style

Zaki MS, Abd-El-All AM, Attia AS, Dahshan H, Al-Ashery MA, Megahed A, . Escherichia coli and Salmonella enterica isolated from Egyptian dairy cattle herds: The prevalence and molecular characteristics. Open Vet J. 2024; 14((1) (Zagazig Veterinary Conference)): 214-224. doi:10.5455/OVJ.2024.v14.i1.19



Vancouver/ICMJE Style

Zaki MS, Abd-El-All AM, Attia AS, Dahshan H, Al-Ashery MA, Megahed A, . Escherichia coli and Salmonella enterica isolated from Egyptian dairy cattle herds: The prevalence and molecular characteristics. Open Vet J. (2024), [cited February 12, 2024]; 14((1) (Zagazig Veterinary Conference)): 214-224. doi:10.5455/OVJ.2024.v14.i1.19



Harvard Style

Zaki, M. S., Abd-El-All, A. M., Attia, A. S., Dahshan, H., Al-Ashery, M. A., Megahed, A. & (2024) Escherichia coli and Salmonella enterica isolated from Egyptian dairy cattle herds: The prevalence and molecular characteristics. Open Vet J, 14 ((1) (Zagazig Veterinary Conference)), 214-224. doi:10.5455/OVJ.2024.v14.i1.19



Turabian Style

Zaki, Mahmoud S.A., Amr M.M. Abd-El-All, Amira S.A. Attia, Hesham Dahshan, Manal A. Al-Ashery, Ayman Megahed, and . 2024. Escherichia coli and Salmonella enterica isolated from Egyptian dairy cattle herds: The prevalence and molecular characteristics. Open Veterinary Journal, 14 ((1) (Zagazig Veterinary Conference)), 214-224. doi:10.5455/OVJ.2024.v14.i1.19



Chicago Style

Zaki, Mahmoud S.A., Amr M.M. Abd-El-All, Amira S.A. Attia, Hesham Dahshan, Manal A. Al-Ashery, Ayman Megahed, and . "Escherichia coli and Salmonella enterica isolated from Egyptian dairy cattle herds: The prevalence and molecular characteristics." Open Veterinary Journal 14 (2024), 214-224. doi:10.5455/OVJ.2024.v14.i1.19



MLA (The Modern Language Association) Style

Zaki, Mahmoud S.A., Amr M.M. Abd-El-All, Amira S.A. Attia, Hesham Dahshan, Manal A. Al-Ashery, Ayman Megahed, and . "Escherichia coli and Salmonella enterica isolated from Egyptian dairy cattle herds: The prevalence and molecular characteristics." Open Veterinary Journal 14.(1) (Zagazig Veterinary Conference) (2024), 214-224. Print. doi:10.5455/OVJ.2024.v14.i1.19



APA (American Psychological Association) Style

Zaki, M. S., Abd-El-All, A. M., Attia, A. S., Dahshan, H., Al-Ashery, M. A., Megahed, A. & (2024) Escherichia coli and Salmonella enterica isolated from Egyptian dairy cattle herds: The prevalence and molecular characteristics. Open Veterinary Journal, 14 ((1) (Zagazig Veterinary Conference)), 214-224. doi:10.5455/OVJ.2024.v14.i1.19