E-ISSN 2218-6050 | ISSN 2226-4485
 

Research Article


Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease

Hinano Eto, Atsushi Yamazaki, Yuma Tomo, Koji Tanegashima, Kazuya Edamura.


Abstract
Background:
Canine Legg Calvé Perthes disease (LCPD) occurs during the growth period, and the cause of ischemic necrosis of the femoral head during growth remains unclear. If LCPD-affected femoral head-derived mesenchymal stem cells (LCPD-MSCs) can be generated, they can be used as a new tool for the pathophysiological analysis of canine LCPD.
Aim:
To generate affected femoral head-derived MSCs from dogs with LCPD and investigate the mRNA expression levels of angiogenesis-related factors and osteogenic differentiation potency of LCPD-MSCs.
Methods:
This study was performed using affected femoral heads from dogs diagnosed with LCPD and underwent femoral head and neck ostectomy. The necrotic tissue was harvested from the LCPD-affected femoral head and cultured statically (LCPD group, n=6). Canine bone marrow-derived MSCs (BM-MSCs) were used as controls (control group, n=6). First, the morphology of the cultured cells was observed, and the expression of CD29, CD34, CD44, CD45, CD90, and major histocompatibility complex class II was analyzed using flow cytometry. Additionally, the trilineage differentiation potency of the LCPD-affected head-derived adherent cells was examined. Furthermore, the expression levels of HIF1A, VEGFA, VEGFB, and PDGFB mRNAs and the bone differentiation potency of LCPD-affected head-derived adherent cells were investigated.
Results:
LCPD-affected femoral head-derived adherent cells showed a fibroblast-like morphology, and the expression of cell surface antigens was similar to that of BM-MSCs. In addition, LCPD-affected femoral head-derived adherent cells showed the same trilineage differentiation potency as BM-MSCs and were consistent with MSC characteristics. Furthermore, the mRNA expression levels of angiogenesis-related factors could be objectively measured in LCPD-MSCs and those MSCs had bone differentiation potency.
Conclusion:
In the present study, canine LCPD-MSCs were successfully generated, suggesting their usefulness as a tool for pathological analysis of LCPD in dogs.

Key words: Angiogenesis related gene, Bone differentiation potency, Dog, Legg Calvé Perthes disease, Mesenchymal stem cell


 
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How to Cite this Article
Pubmed Style

HE, Yamazaki A, Tomo Y, Tanegashima K, Edamura K. Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease. Open Vet J. 2024; 14(5): 1172-1181. doi:10.5455/OVJ.2024.v14.i5.12


Web Style

HE, Yamazaki A, Tomo Y, Tanegashima K, Edamura K. Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease. https://www.openveterinaryjournal.com/?mno=189217 [Access: June 04, 2024]. doi:10.5455/OVJ.2024.v14.i5.12


AMA (American Medical Association) Style

HE, Yamazaki A, Tomo Y, Tanegashima K, Edamura K. Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease. Open Vet J. 2024; 14(5): 1172-1181. doi:10.5455/OVJ.2024.v14.i5.12



Vancouver/ICMJE Style

HE, Yamazaki A, Tomo Y, Tanegashima K, Edamura K. Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease. Open Vet J. (2024), [cited June 04, 2024]; 14(5): 1172-1181. doi:10.5455/OVJ.2024.v14.i5.12



Harvard Style

, H. E., Yamazaki, . A., Tomo, . Y., Tanegashima, . K. & Edamura, . K. (2024) Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease. Open Vet J, 14 (5), 1172-1181. doi:10.5455/OVJ.2024.v14.i5.12



Turabian Style

, Hinano Eto, Atsushi Yamazaki, Yuma Tomo, Koji Tanegashima, and Kazuya Edamura. 2024. Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease. Open Veterinary Journal, 14 (5), 1172-1181. doi:10.5455/OVJ.2024.v14.i5.12



Chicago Style

, Hinano Eto, Atsushi Yamazaki, Yuma Tomo, Koji Tanegashima, and Kazuya Edamura. "Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease." Open Veterinary Journal 14 (2024), 1172-1181. doi:10.5455/OVJ.2024.v14.i5.12



MLA (The Modern Language Association) Style

, Hinano Eto, Atsushi Yamazaki, Yuma Tomo, Koji Tanegashima, and Kazuya Edamura. "Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease." Open Veterinary Journal 14.5 (2024), 1172-1181. Print. doi:10.5455/OVJ.2024.v14.i5.12



APA (American Psychological Association) Style

, H. E., Yamazaki, . A., Tomo, . Y., Tanegashima, . K. & Edamura, . K. (2024) Generation and characterization of mesenchymal stem cells from the affected femoral heads of dogs with Legg Calvé Perthes disease. Open Veterinary Journal, 14 (5), 1172-1181. doi:10.5455/OVJ.2024.v14.i5.12