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Research Article


Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity

Gabriel Martín Alvarez, Sofía Villanueva, Elizabeth Breininger, Marisa Geller, Claudio Ruhlmann, Gabriel Carlos Dalvit, Pablo Daniel Cetica, Masashige Kuwayama.


Abstract
Background:
Intracytoplasmic sperm injection (ICSI) technique has low efficiency in cattle. This has mainly been attributed to the oocyte activation failure due to oocyte and/or sperm factors.
Aim:
Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with PLCɀ activity of both species.
Methods:
In vitro matured bovine oocytes were randomly divided into five groups and were fertilized as follows: conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCɀ activity was determined in bull and human sperm samples.
Results:
Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages, and ICSI + chemical activation presented the highest 2 PN, cleavage and blastocyst rates (P<0.05). Within the groups using human sperm, the oocytes fertilized by Piezo-ICSI presented higher 2 PN and cleavage rates than those activated by conventional ICSI (P<0.05). Piezo-ICSI with human sperm increased bovine oocyte activation as much as conventional ICSI + chemical activation with bovine sperm (P<0.05). Higher values of PLCɀ activity were found in human sperm compared with bovine sperm (P<0.05).
Conclusion:
Our results suggest that the higher stability of the bovine sperm in combination with its relative low content of PLCɀ impair bovine oocyte activation after ICSI.

Key words: Bovine, ICSI, Piezo-ICSI, PLCɀ, Oocyte activation


 
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How to Cite this Article
Pubmed Style

Alvarez GM, Villanueva S, Breininger E, MG, Ruhlmann C, Dalvit GC, Cetica PD, Kuwayama M. Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity. Open Vet J. 2024; 14(5): 1191-1198. doi:10.5455/OVJ.2024.v14.i5.14


Web Style

Alvarez GM, Villanueva S, Breininger E, MG, Ruhlmann C, Dalvit GC, Cetica PD, Kuwayama M. Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity. https://www.openveterinaryjournal.com/?mno=190174 [Access: June 04, 2024]. doi:10.5455/OVJ.2024.v14.i5.14


AMA (American Medical Association) Style

Alvarez GM, Villanueva S, Breininger E, MG, Ruhlmann C, Dalvit GC, Cetica PD, Kuwayama M. Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity. Open Vet J. 2024; 14(5): 1191-1198. doi:10.5455/OVJ.2024.v14.i5.14



Vancouver/ICMJE Style

Alvarez GM, Villanueva S, Breininger E, MG, Ruhlmann C, Dalvit GC, Cetica PD, Kuwayama M. Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity. Open Vet J. (2024), [cited June 04, 2024]; 14(5): 1191-1198. doi:10.5455/OVJ.2024.v14.i5.14



Harvard Style

Alvarez, G. M., Villanueva, . S., Breininger, . E., , . M. G., Ruhlmann, . C., Dalvit, . G. C., Cetica, . P. D. & Kuwayama, . M. (2024) Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity. Open Vet J, 14 (5), 1191-1198. doi:10.5455/OVJ.2024.v14.i5.14



Turabian Style

Alvarez, Gabriel Martín, Sofía Villanueva, Elizabeth Breininger, Marisa Geller, Claudio Ruhlmann, Gabriel Carlos Dalvit, Pablo Daniel Cetica, and Masashige Kuwayama. 2024. Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity. Open Veterinary Journal, 14 (5), 1191-1198. doi:10.5455/OVJ.2024.v14.i5.14



Chicago Style

Alvarez, Gabriel Martín, Sofía Villanueva, Elizabeth Breininger, Marisa Geller, Claudio Ruhlmann, Gabriel Carlos Dalvit, Pablo Daniel Cetica, and Masashige Kuwayama. "Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity." Open Veterinary Journal 14 (2024), 1191-1198. doi:10.5455/OVJ.2024.v14.i5.14



MLA (The Modern Language Association) Style

Alvarez, Gabriel Martín, Sofía Villanueva, Elizabeth Breininger, Marisa Geller, Claudio Ruhlmann, Gabriel Carlos Dalvit, Pablo Daniel Cetica, and Masashige Kuwayama. "Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity." Open Veterinary Journal 14.5 (2024), 1191-1198. Print. doi:10.5455/OVJ.2024.v14.i5.14



APA (American Psychological Association) Style

Alvarez, G. M., Villanueva, . S., Breininger, . E., , . M. G., Ruhlmann, . C., Dalvit, . G. C., Cetica, . P. D. & Kuwayama, . M. (2024) Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCɀ activity. Open Veterinary Journal, 14 (5), 1191-1198. doi:10.5455/OVJ.2024.v14.i5.14