E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 8
14 / 8Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Research Article


Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya

Fawzia Mohamed, Ayiman Aboulqassim, Monier Sharif, Salh Belgasem, Abraheem Omar, Nagi Saeed.


Abstract
Background:
The most common natural prion disease that primarily affects sheep and goats is scrapie. It belongs to a group of disorders known as transmissible spongiform encephalopathies (TSEs), which impact both humans and animals.
Aim:
The research is aimed to examine and confirm the presence of Scrapie in Libya using
immunohistochemistry (IHC) techniques.
Methods:
Brain samples were collected from thirty-three sheep older than two years of age showing clinical signs resembling to scrapie during the period between 2018 to 2023, regardless of race or gender. Three animals, six months old, healthy, and without any symptoms, were used as negative controls. Different parts of the brain, including obex and cerebellum, were taken from each case. The IHC technique used in this study involved staining with monoclonal antibody L42 and DAB (3,3′-diaminobenzidine) as a chromogenic substrate.
Results:
The IHC examination showed the expression of prion proteins in brain tissue in twenty-three samples. The staining intensity was markedly observed in the neuronal cell bodies and around blood vessels.
Conclusion:
The findings of this study provide evidence that Scrapie exists in Libya.

Key words: Immunohistochemistry, Libya, Prion, Scrapie, Sheep


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Fawzia Mohamed
Articles by Ayiman Aboulqassim
Articles by Monier Sharif
Articles by Salh Belgasem
Articles by Abraheem Omar
Articles by Nagi Saeed
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Mohamed F, Aboulqassim A, Sharif M, Belgasem S, Omar A, Saeed N. Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Vet J. 2024; 14(8): 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12

Web Style

Mohamed F, Aboulqassim A, Sharif M, Belgasem S, Omar A, Saeed N. Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. https://www.openveterinaryjournal.com/?mno=197391 [Access: September 16, 2024]. doi:10.5455/OVJ.2024.v14.i8.12

AMA (American Medical Association) Style

Mohamed F, Aboulqassim A, Sharif M, Belgasem S, Omar A, Saeed N. Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Vet J. 2024; 14(8): 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


Vancouver/ICMJE Style

Mohamed F, Aboulqassim A, Sharif M, Belgasem S, Omar A, Saeed N. Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Vet J. (2024), [cited September 16, 2024]; 14(8): 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


Harvard Style

Mohamed, F., Aboulqassim, . A., Sharif, . M., Belgasem, . S., Omar, . A. & Saeed, . N. (2024) Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Vet J, 14 (8), 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


Turabian Style

Mohamed, Fawzia, Ayiman Aboulqassim, Monier Sharif, Salh Belgasem, Abraheem Omar, and Nagi Saeed. 2024. Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Veterinary Journal, 14 (8), 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


Chicago Style

Mohamed, Fawzia, Ayiman Aboulqassim, Monier Sharif, Salh Belgasem, Abraheem Omar, and Nagi Saeed. "Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya." Open Veterinary Journal 14 (2024), 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


MLA (The Modern Language Association) Style

Mohamed, Fawzia, Ayiman Aboulqassim, Monier Sharif, Salh Belgasem, Abraheem Omar, and Nagi Saeed. "Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya." Open Veterinary Journal 14.8 (2024), 1843-1849. Print. doi:10.5455/OVJ.2024.v14.i8.12


APA (American Psychological Association) Style

Mohamed, F., Aboulqassim, . A., Sharif, . M., Belgasem, . S., Omar, . A. & Saeed, . N. (2024) Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Veterinary Journal, 14 (8), 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly