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Research Article


Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells

Agung Budianto Achmad, Annise Proboningrat, Arif Nur Muhammad Ansori, Amaq Fadholly, Siti Eliana Rochmi, Rinza Rahmawati Samsudin, Nanik Hidayatik, Gracia Angelina Hendarti, Shara Jayanti.


Abstract
Background:
Cervical cancer is a severe concern for women throughout the world. This percentage of cancer incidence causes sufferers to die at a high rate. It is believed that the bark of the Pinus merkusii tree contains anti-cancer compounds that inhibit cervical cancer cell growth.

Aim:
This present study aims to examine the cytotoxic ability of P. merkusii tree bark ethanol extract (PMBE) by inducing apoptosis in HeLa cells.

Methods:
We administered the PMBE at concentrations of 50, 100, 200, and 400 µg/mL to HeLa cell cultures. We then conducted the MTT cytotoxicity assay, detected apoptosis via Annexin V binding, and observed caspase 9 expression via immunocytochemistry.

Results:
PMBE showed cytotoxic activity on HeLa cells with an IC50 of 226.6 µg/mL for 24 hours of treatment. PMBE caused early apoptosis in up to 81.31% of HeLa cells, as well as increased caspase-9 expression.

Conclusion:
Based on this study, PMBE is predicted to have dose-dependent antiproliferative or cytotoxicity effects on the HeLa cell line through the intrinsic pathway apoptosis mechanism.

Key words: Anticancer, Cervical cancer, Apoptosis, Pinaceae


 
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How to Cite this Article
Pubmed Style

Achmad AB, Proboningrat A, Ansori ANM, Fadholly A, Rochmi SE, Samsudin RR, Hidayatik N, Hendarti GA, Jayanti S. Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells. Open Vet J. 2024; 14(10): 2628-2633. doi:10.5455/OVJ.2024.v14.i10.12

Web Style

Achmad AB, Proboningrat A, Ansori ANM, Fadholly A, Rochmi SE, Samsudin RR, Hidayatik N, Hendarti GA, Jayanti S. Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells. https://www.openveterinaryjournal.com/?mno=208671 [Access: October 30, 2024]. doi:10.5455/OVJ.2024.v14.i10.12

AMA (American Medical Association) Style

Achmad AB, Proboningrat A, Ansori ANM, Fadholly A, Rochmi SE, Samsudin RR, Hidayatik N, Hendarti GA, Jayanti S. Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells. Open Vet J. 2024; 14(10): 2628-2633. doi:10.5455/OVJ.2024.v14.i10.12


Vancouver/ICMJE Style

Achmad AB, Proboningrat A, Ansori ANM, Fadholly A, Rochmi SE, Samsudin RR, Hidayatik N, Hendarti GA, Jayanti S. Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells. Open Vet J. (2024), [cited October 30, 2024]; 14(10): 2628-2633. doi:10.5455/OVJ.2024.v14.i10.12


Harvard Style

Achmad, A. B., Proboningrat, . A., Ansori, . A. N. M., Fadholly, . A., Rochmi, . S. E., Samsudin, . R. R., Hidayatik, . N., Hendarti, . G. A. & Jayanti, . S. (2024) Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells. Open Vet J, 14 (10), 2628-2633. doi:10.5455/OVJ.2024.v14.i10.12


Turabian Style

Achmad, Agung Budianto, Annise Proboningrat, Arif Nur Muhammad Ansori, Amaq Fadholly, Siti Eliana Rochmi, Rinza Rahmawati Samsudin, Nanik Hidayatik, Gracia Angelina Hendarti, and Shara Jayanti. 2024. Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells. Open Veterinary Journal, 14 (10), 2628-2633. doi:10.5455/OVJ.2024.v14.i10.12


Chicago Style

Achmad, Agung Budianto, Annise Proboningrat, Arif Nur Muhammad Ansori, Amaq Fadholly, Siti Eliana Rochmi, Rinza Rahmawati Samsudin, Nanik Hidayatik, Gracia Angelina Hendarti, and Shara Jayanti. "Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells." Open Veterinary Journal 14 (2024), 2628-2633. doi:10.5455/OVJ.2024.v14.i10.12


MLA (The Modern Language Association) Style

Achmad, Agung Budianto, Annise Proboningrat, Arif Nur Muhammad Ansori, Amaq Fadholly, Siti Eliana Rochmi, Rinza Rahmawati Samsudin, Nanik Hidayatik, Gracia Angelina Hendarti, and Shara Jayanti. "Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells." Open Veterinary Journal 14.10 (2024), 2628-2633. Print. doi:10.5455/OVJ.2024.v14.i10.12


APA (American Psychological Association) Style

Achmad, A. B., Proboningrat, . A., Ansori, . A. N. M., Fadholly, . A., Rochmi, . S. E., Samsudin, . R. R., Hidayatik, . N., Hendarti, . G. A. & Jayanti, . S. (2024) Stem bark ethanolic extract of Pinus merkusii induces caspase 9-mediated apoptosis in HeLa cells. Open Veterinary Journal, 14 (10), 2628-2633. doi:10.5455/OVJ.2024.v14.i10.12


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