Abstract
Background:
Cisplatin is a highly effective chemotherapeutic drug. However, it is associated with various side effects, including kidney damage, due to its nephrotoxic properties.

Aim:
This study aimed to evaluate the renoprotective potential of the combined extract of Curcuma longa and Curcuma zedoaria in reducing nephrotoxicity by examining its effects on TNF-α, KIM-1, and Caspase-3 levels.

Methods:
Twenty-five rats were divided into normal control groups (NS), cisplatin control groups (CIS), and three treatment groups that received doses of the combined extract at 100, 200, and 400 mg/kg (CUR100, CUR200, and CUR400), respectively on day 1-20. All groups, except the NS group (receiving normal saline i. p.), received intraperitoneal cisplatin (1 mg/kg) on days 7 and 14 of the 20-day extract treatment.

Results:
Compared with the rats in the CIS group, rats given the combined extract had a considerable gain in body weight and decreased TNF-α, KIM-1, and caspase-3 expression levels. Histopathological examination revealed that the extract group experienced less kidney damage than the CIS group. The combined extract, administered at 200 mg/kg, dexertedthe most apparent protective effect, decreasing renal TNF-α, KIM-1,, and caspase 3.

Conclusion:
The combined extract of Curcuma longa and Curcuma zedoaria has the potential to be a therapeutic agent for reducing nephrotoxicity by suppressing TNF-α, KIM-1, and caspase-3 levels. Further research is required to determine the potential of this combination therapy in humans.

Key words: Cisplatin, Curcuma longa, Curcuma zedoaria, Inflammation, Nephrotoxicity