E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Phylogenetic analysis of some Newcastle disease virus isolates from the Sudan

N.A. Elmardi, M.A. Bakheit, A.I. Khalafalla.

Abstract
A reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify 1412 bp of the fusion protein gene (F gene) of four Newcastle disease virus (NDV) isolates; two velogenic (TY-1/90 and DIK-90) and two lentogenic isolates (Dongla 88/1 and GD.S.1). Following sequencing, nucleotide sequences were annotated and 894 bp were compared phylogenetically with those from strains previously reported in the Sudan and the virus strains published on the GenBank. It could be demonstrated that TY-1/90 and DIK-90 strains belong to the genotype VI of NDV and are in close genetic relationship to sub- genotype VIb. TY-1/90 and DIK-90 strains were observed to be genetically unrelated to the earlier Sudanese isolates of 1970/80s and the late of 2000s suggesting a different origin. The close genetic relationship to the European and African pigeon paramyxovirus type 1 (PPMV-1) suggests a common ancestor. Dongola, GD.S.1 strains were classified into genotype II that comprises non-pathogenic lentogenic NDV strains. The present genetic classification of NDV isolates of the Sudan provides valuable information on genotypes of NDV. Further molecular epidemiological investigations of the recent outbreaks of Newcastle disease in the Sudan are needed in order to improve the efficiency of control strategies and vaccine development.

Key words: Newcastle disease virus, Phylogenetic analysis, Sudan isolates.


 
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How to Cite this Article
Pubmed Style

N.A. Elmardi, M.A. Bakheit, A.I. Khalafalla. Phylogenetic analysis of some Newcastle disease virus isolates from the Sudan. Open Vet J. 2016; 6(2): 89-97. doi:10.4314/ovj.v6i2.4


Web Style

N.A. Elmardi, M.A. Bakheit, A.I. Khalafalla. Phylogenetic analysis of some Newcastle disease virus isolates from the Sudan. https://www.openveterinaryjournal.com/?mno=233988 [Access: November 28, 2022]. doi:10.4314/ovj.v6i2.4


AMA (American Medical Association) Style

N.A. Elmardi, M.A. Bakheit, A.I. Khalafalla. Phylogenetic analysis of some Newcastle disease virus isolates from the Sudan. Open Vet J. 2016; 6(2): 89-97. doi:10.4314/ovj.v6i2.4



Vancouver/ICMJE Style

N.A. Elmardi, M.A. Bakheit, A.I. Khalafalla. Phylogenetic analysis of some Newcastle disease virus isolates from the Sudan. Open Vet J. (2016), [cited November 28, 2022]; 6(2): 89-97. doi:10.4314/ovj.v6i2.4



Harvard Style

N.A. Elmardi, M.A. Bakheit, A.I. Khalafalla (2016) Phylogenetic analysis of some Newcastle disease virus isolates from the Sudan. Open Vet J, 6 (2), 89-97. doi:10.4314/ovj.v6i2.4



Turabian Style

N.A. Elmardi, M.A. Bakheit, A.I. Khalafalla. 2016. Phylogenetic analysis of some Newcastle disease virus isolates from the Sudan. Open Veterinary Journal, 6 (2), 89-97. doi:10.4314/ovj.v6i2.4



Chicago Style

N.A. Elmardi, M.A. Bakheit, A.I. Khalafalla. "Phylogenetic analysis of some Newcastle disease virus isolates from the Sudan." Open Veterinary Journal 6 (2016), 89-97. doi:10.4314/ovj.v6i2.4



MLA (The Modern Language Association) Style

N.A. Elmardi, M.A. Bakheit, A.I. Khalafalla. "Phylogenetic analysis of some Newcastle disease virus isolates from the Sudan." Open Veterinary Journal 6.2 (2016), 89-97. Print. doi:10.4314/ovj.v6i2.4



APA (American Psychological Association) Style

N.A. Elmardi, M.A. Bakheit, A.I. Khalafalla (2016) Phylogenetic analysis of some Newcastle disease virus isolates from the Sudan. Open Veterinary Journal, 6 (2), 89-97. doi:10.4314/ovj.v6i2.4