E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study

A.G. Pose, E.S. Rodríguez, A.C. Méndez, J.N. Gómez, A.V. Redondo, E.R. Rodríguez, E.M.G. Ramos, A.Á. Gutiérrez, M.P.R. Moltó, D.G. Roche, Y.S. Ugalde, A.M. López.

Abstract
In this study we demonstrated that the vaccine candidate against avian influenza virus H5N1 based on the hemagglutinin H5 (HA) fused to the chicken CD154 (HACD) can also be used for differentiating infected from vaccinated animals (DIVA). As the strategy of DIVA requires at least two proteins, we obtained a variant of the nucleoprotein (NP49-375) in E. coli. After its purification by IMAC, the competence of the proteins NP49-375 and HACD as coating antigens in indirect ELISA assays were tested by using the sera of chickens immunized with the proteins HA and HACD and the reference sera from several avian influenza subtypes. Together with these sera, the sera from different species of birds and the sera of chickens infected with other avian viral diseases were analyzed by competition ELISA assays coated with the proteins NP49-375 and HACD. The results showed that the segment CD154 in the chimeric protein HACD did not interfere with the recognition of the molecule HA by its specific antibodies. Also, we observed variable detection levels when the reference sera were analyzed in the ELISA plates coated with the protein NP49-375. Moreover, only the antibodies of the reference serum subtype H5 were detected in the ELISA plates coated with the protein HACD. The competition ELISA assays showed percentages of inhibition of 88-91% for the positives sera and less than 20% for the negative sera. We fixed the cut-off value of these assays at 25%. No antibody detection was observed in the sera from different species of birds or the sera of chickens infected with other avian viral diseases. This study supported the fact that the ELISA assays using the proteins NP49-375 and HACD could be valuable tools for avian influenza surveillance and as a strategy of DIVA for counteracting the highly pathogenic avian influenza virus H5N1 outbreaks.

Key words: Avian influenza virus, CD154, Competition ELISA, DIVA, Hemagglutinin.


 
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How to Cite this Article
Pubmed Style

A.G. Pose, E.S. Rodríguez, A.C. Méndez, J.N. Gómez, A.V. Redondo, E.R. Rodríguez, E.M.G. Ramos, A.Á. Gutiérrez, M.P.R. Moltó, D.G. Roche, Y.S. Ugalde, A.M. López. Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study. Open Vet J. 2015; 5(2): 138-147. doi:10.5455/OVJ.2015.v5.i2.p138


Web Style

A.G. Pose, E.S. Rodríguez, A.C. Méndez, J.N. Gómez, A.V. Redondo, E.R. Rodríguez, E.M.G. Ramos, A.Á. Gutiérrez, M.P.R. Moltó, D.G. Roche, Y.S. Ugalde, A.M. López. Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study. https://www.openveterinaryjournal.com/?mno=235331 [Access: February 07, 2023]. doi:10.5455/OVJ.2015.v5.i2.p138


AMA (American Medical Association) Style

A.G. Pose, E.S. Rodríguez, A.C. Méndez, J.N. Gómez, A.V. Redondo, E.R. Rodríguez, E.M.G. Ramos, A.Á. Gutiérrez, M.P.R. Moltó, D.G. Roche, Y.S. Ugalde, A.M. López. Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study. Open Vet J. 2015; 5(2): 138-147. doi:10.5455/OVJ.2015.v5.i2.p138



Vancouver/ICMJE Style

A.G. Pose, E.S. Rodríguez, A.C. Méndez, J.N. Gómez, A.V. Redondo, E.R. Rodríguez, E.M.G. Ramos, A.Á. Gutiérrez, M.P.R. Moltó, D.G. Roche, Y.S. Ugalde, A.M. López. Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study. Open Vet J. (2015), [cited February 07, 2023]; 5(2): 138-147. doi:10.5455/OVJ.2015.v5.i2.p138



Harvard Style

A.G. Pose, E.S. Rodríguez, A.C. Méndez, J.N. Gómez, A.V. Redondo, E.R. Rodríguez, E.M.G. Ramos, A.Á. Gutiérrez, M.P.R. Moltó, D.G. Roche, Y.S. Ugalde, A.M. López (2015) Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study. Open Vet J, 5 (2), 138-147. doi:10.5455/OVJ.2015.v5.i2.p138



Turabian Style

A.G. Pose, E.S. Rodríguez, A.C. Méndez, J.N. Gómez, A.V. Redondo, E.R. Rodríguez, E.M.G. Ramos, A.Á. Gutiérrez, M.P.R. Moltó, D.G. Roche, Y.S. Ugalde, A.M. López. 2015. Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study. Open Veterinary Journal, 5 (2), 138-147. doi:10.5455/OVJ.2015.v5.i2.p138



Chicago Style

A.G. Pose, E.S. Rodríguez, A.C. Méndez, J.N. Gómez, A.V. Redondo, E.R. Rodríguez, E.M.G. Ramos, A.Á. Gutiérrez, M.P.R. Moltó, D.G. Roche, Y.S. Ugalde, A.M. López. "Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study." Open Veterinary Journal 5 (2015), 138-147. doi:10.5455/OVJ.2015.v5.i2.p138



MLA (The Modern Language Association) Style

A.G. Pose, E.S. Rodríguez, A.C. Méndez, J.N. Gómez, A.V. Redondo, E.R. Rodríguez, E.M.G. Ramos, A.Á. Gutiérrez, M.P.R. Moltó, D.G. Roche, Y.S. Ugalde, A.M. López. "Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study." Open Veterinary Journal 5.2 (2015), 138-147. Print. doi:10.5455/OVJ.2015.v5.i2.p138



APA (American Psychological Association) Style

A.G. Pose, E.S. Rodríguez, A.C. Méndez, J.N. Gómez, A.V. Redondo, E.R. Rodríguez, E.M.G. Ramos, A.Á. Gutiérrez, M.P.R. Moltó, D.G. Roche, Y.S. Ugalde, A.M. López (2015) Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study. Open Veterinary Journal, 5 (2), 138-147. doi:10.5455/OVJ.2015.v5.i2.p138