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Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt

Mahmoud E R Hamdy, Hoda M Zaki.


Cited By:15

Abstract
The current study involved detection of three virulence genes (bvfA, virB, ure) by PCR in 52 isolates of Brucella melitensis biovar 3, recovered from different animal species (29 sheep, 10 goats, 9 cattle and 5 buffaloes). Of the 52 B. melitensis strains; 48 (92.3%) isolates carried bvfA genes, 51 (98.1%) isolates had virB genes and 50 (96.2%) isolates were positive for ure genes.
The distribution of the virulence genes is not affected by crossing the original host barriers of the animal species, as the three virulence factors (bvfA, virB and ure) detected in 29 B. melitensis isolates obtained from ovine species in a ratio of 27/29 (93.1%), 28/29 (96.6%) and 29/29 (100%), respectively. While 10 isolates originating from goats revealed a ratio of 10/10 (100%), 10/10 (100%) and 9/10 (90%) to the same order of virulence genes. Nearly, similar results of virulence genes detection were obtained in B. melitensis obtained from bovine (8/9, 9/9 and 8/9) and Buffalos (4/5, 5/5 and 5/5), respectively. The high prevalence of virulence-associated genes among the B. melitensis isolates detected from different animal species in Egypt is indicated a potential virulence of this bacterium.

Key words: Brucella melitensis biovar.3 – bvfA – virB - ure – PCR


 
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Pubmed Style

Mahmoud E R Hamdy, Hoda M Zaki. Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Vet J. 2018; 8(1): 112-117. doi:10.4314/ovj.v8i1.17

Web Style

Mahmoud E R Hamdy, Hoda M Zaki. Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. https://www.openveterinaryjournal.com/?mno=286052 [Access: December 06, 2024]. doi:10.4314/ovj.v8i1.17

AMA (American Medical Association) Style

Mahmoud E R Hamdy, Hoda M Zaki. Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Vet J. 2018; 8(1): 112-117. doi:10.4314/ovj.v8i1.17


Vancouver/ICMJE Style

Mahmoud E R Hamdy, Hoda M Zaki. Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Vet J. (2018), [cited December 06, 2024]; 8(1): 112-117. doi:10.4314/ovj.v8i1.17


Harvard Style

Mahmoud E R Hamdy, Hoda M Zaki (2018) Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Vet J, 8 (1), 112-117. doi:10.4314/ovj.v8i1.17


Turabian Style

Mahmoud E R Hamdy, Hoda M Zaki. 2018. Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Veterinary Journal, 8 (1), 112-117. doi:10.4314/ovj.v8i1.17


Chicago Style

Mahmoud E R Hamdy, Hoda M Zaki. "Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt." Open Veterinary Journal 8 (2018), 112-117. doi:10.4314/ovj.v8i1.17


MLA (The Modern Language Association) Style

Mahmoud E R Hamdy, Hoda M Zaki. "Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt." Open Veterinary Journal 8.1 (2018), 112-117. Print. doi:10.4314/ovj.v8i1.17


APA (American Psychological Association) Style

Mahmoud E R Hamdy, Hoda M Zaki (2018) Detection of virulence-associated genes in Brucella melitensis biovar 3, the prevalent field Strain in different animal species in Egypt. Open Veterinary Journal, 8 (1), 112-117. doi:10.4314/ovj.v8i1.17


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