Abstract
Background:
Wound healing continues to pose a considerable clinical challenge, especially in the context of surgical intervention, despite numerous treatment options; an ideal solution for wound injuries has yet to be established. Small intestinal submucosa-extracellular matrix (SIS-ECM) has shown potential in regenerative medicine due to its properties that may facilitate tissue repair and wound healing.

Aim:
This study aims to assess the efficacy of SIS-ECM powder in promoting the healing of full-thickness wounds in the rabbit model, with particular emphasis on focusing on epithelialization rate and inflammatory response reduction.

Methods:
Twenty clinically healthy adult male rabbits were allocated into two experimental groups. A full-thickness excisional wounds measuring 2 × 2 cm was surgically induced on the dorsal skin of each animal. Group A (control group) received phosphate-buffered saline, whereas Group B (treatment group) was administered SIS-ECM powder. Histopathological evaluation was conducted via biopsy on days 7th, 14th, and 35th post wounding to monitor healing progression.

Results:
Topical application SIS-ECM powder-significantly accelerated epithelialization by day 21st of treatment (p < 0.05) and markedly reduced inflammation within the week signs within the first week (p < 0. 01). Compared to the control group These statistically significant findings suggest that SIS-ECM powder fosters a supportive microenvironment that conductive to cellular proliferation, and differentiation, thereby enhancing the repair of full-thickness wounds.

Conclusion:
SIS-ECM powder creates a favorable microenviroment that supports cell growth and differentiation, thereby enhancing the healing process of full-thickness wounds.

Key words: Healing; Intestine; Rabbit; SIS-ECM powder; Wounds.