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Research Article


Effect of Moringa oleifera leaf extract supplementation on motility and viability of Boer crossbreed buck spermatozoa during cold storage

Rahmat Rahmat, Muhammad Rizal, Nursyam Andi Syarifuddin, Muhammad Riyadhi, Athhar Manabi Diansyah, Muhammad Fajar Amrullah, Andi Ni'mahtul Churriyah, Anis Wahdi, Ahmad Alfaruqi Syahrandi Adam, Andi Muhammad Alfian, Muhammad Rizal.


Abstract
Background:
Chilled semen preservation is essential for supporting artificial insemination (AI) programs in Boer goats, but spermatozoa rapidly deteriorate during cold storage due to cold shock and oxidative stress. Natural antioxidant such as Moringa oleifera leaf extract (MLE) which contains flavonoids, phenolics and vitamin may enhance extender performance for maintaining semen quality by reducing oxidative damage. However, Studies evaluating MLE supplementation in commercial extenders such as Andromed® are still limited and have not beend widely conducted, particularly for Boer goat semen stored at 5 °C.

Aim:
This study aims to evaluate the effect of different concentrations of Moringa oleifera leaf extract supplementation (2%, 4%, and 6%) in Andromed® extender on the motility and viability of Boer goat sperm during five days of chilled storage.

Methods:
Semen from six Boer goats was diluted with Andromed® containing 0% (T0 as control), 2% (T1), 4% (T2) and 6% (T3) MLE and stored at 5°C for 5 days. Sperm motility and viability were assessed daily using eosin-nigrosin staining.

Results:
Sperm motility and viability shoved distinct responses among treatments during storage. The 4% (T2) MLE treatment consistently maintained the highest sperm motility and viability from Day 2 onward. On Day 5, T2 exhibited the greatest motility (40.00 ± 0.00%) and viability (51.00 ± 2.16%), significantly outperforming T0, T1 and T3. Increasing the MLE concentrations to 6% did not provide further improvement and resulted in lower values than T2, suggesting that excessive extract may exert negative effects.

Conclusion:
In conclusion, supplementation of 4% Moringa oleifera leaf extract in Andromed® extender effectively preserves sperm motility and viability in chilled Boer goat semen. MLE serves as a promising natural antioxidant additive for improving short- term semen preservation, particulary under tropical conditions.

Key words: Antioxidant supplementation; Boer buck; Chilled semen; Moringa oleifera leaf extract; Semen extender.


 
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How to Cite this Article
Pubmed Style

Rahmat R, Rizal M, Syarifuddin NA, Riyadhi M, Diansyah AM, Amrullah MF, Churriyah AN, Wahdi A, Adam AAS, Alfian AM, Rizal M. Effect of Moringa oleifera leaf extract supplementation on motility and viability of Boer crossbreed buck spermatozoa during cold storage. Open Vet. J.. 2026; 16(5): 2823-2830. doi:10.5455/OVJ.2026.v16.i5.24

Web Style

Rahmat R, Rizal M, Syarifuddin NA, Riyadhi M, Diansyah AM, Amrullah MF, Churriyah AN, Wahdi A, Adam AAS, Alfian AM, Rizal M. Effect of Moringa oleifera leaf extract supplementation on motility and viability of Boer crossbreed buck spermatozoa during cold storage. https://www.openveterinaryjournal.com/?mno=299211 [Access: June 10, 2026]. doi:10.5455/OVJ.2026.v16.i5.24

AMA (American Medical Association) Style

Rahmat R, Rizal M, Syarifuddin NA, Riyadhi M, Diansyah AM, Amrullah MF, Churriyah AN, Wahdi A, Adam AAS, Alfian AM, Rizal M. Effect of Moringa oleifera leaf extract supplementation on motility and viability of Boer crossbreed buck spermatozoa during cold storage. Open Vet. J.. 2026; 16(5): 2823-2830. doi:10.5455/OVJ.2026.v16.i5.24


Vancouver/ICMJE Style

Rahmat R, Rizal M, Syarifuddin NA, Riyadhi M, Diansyah AM, Amrullah MF, Churriyah AN, Wahdi A, Adam AAS, Alfian AM, Rizal M. Effect of Moringa oleifera leaf extract supplementation on motility and viability of Boer crossbreed buck spermatozoa during cold storage. Open Vet. J.. (2026), [cited June 10, 2026]; 16(5): 2823-2830. doi:10.5455/OVJ.2026.v16.i5.24


Harvard Style

Rahmat, R., Rizal, . M., Syarifuddin, . N. A., Riyadhi, . M., Diansyah, . A. M., Amrullah, . M. F., Churriyah, . A. N., Wahdi, . A., Adam, . A. A. S., Alfian, . A. M. & Rizal, . M. (2026) Effect of Moringa oleifera leaf extract supplementation on motility and viability of Boer crossbreed buck spermatozoa during cold storage. Open Vet. J., 16 (5), 2823-2830. doi:10.5455/OVJ.2026.v16.i5.24


Turabian Style

Rahmat, Rahmat, Muhammad Rizal, Nursyam Andi Syarifuddin, Muhammad Riyadhi, Athhar Manabi Diansyah, Muhammad Fajar Amrullah, Andi Nimahtul Churriyah, Anis Wahdi, Ahmad Alfaruqi Syahrandi Adam, Andi Muhammad Alfian, and Muhammad Rizal. 2026. Effect of Moringa oleifera leaf extract supplementation on motility and viability of Boer crossbreed buck spermatozoa during cold storage. Open Veterinary Journal, 16 (5), 2823-2830. doi:10.5455/OVJ.2026.v16.i5.24


Chicago Style

Rahmat, Rahmat, Muhammad Rizal, Nursyam Andi Syarifuddin, Muhammad Riyadhi, Athhar Manabi Diansyah, Muhammad Fajar Amrullah, Andi Nimahtul Churriyah, Anis Wahdi, Ahmad Alfaruqi Syahrandi Adam, Andi Muhammad Alfian, and Muhammad Rizal. "Effect of Moringa oleifera leaf extract supplementation on motility and viability of Boer crossbreed buck spermatozoa during cold storage." Open Veterinary Journal 16 (2026), 2823-2830. doi:10.5455/OVJ.2026.v16.i5.24


MLA (The Modern Language Association) Style

Rahmat, Rahmat, Muhammad Rizal, Nursyam Andi Syarifuddin, Muhammad Riyadhi, Athhar Manabi Diansyah, Muhammad Fajar Amrullah, Andi Nimahtul Churriyah, Anis Wahdi, Ahmad Alfaruqi Syahrandi Adam, Andi Muhammad Alfian, and Muhammad Rizal. "Effect of Moringa oleifera leaf extract supplementation on motility and viability of Boer crossbreed buck spermatozoa during cold storage." Open Veterinary Journal 16.5 (2026), 2823-2830. Print. doi:10.5455/OVJ.2026.v16.i5.24


APA (American Psychological Association) Style

Rahmat, R., Rizal, . M., Syarifuddin, . N. A., Riyadhi, . M., Diansyah, . A. M., Amrullah, . M. F., Churriyah, . A. N., Wahdi, . A., Adam, . A. A. S., Alfian, . A. M. & Rizal, . M. (2026) Effect of Moringa oleifera leaf extract supplementation on motility and viability of Boer crossbreed buck spermatozoa during cold storage. Open Veterinary Journal, 16 (5), 2823-2830. doi:10.5455/OVJ.2026.v16.i5.24


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