E-ISSN 2218-6050 | ISSN 2226-4485
 

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Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan

A.E. El Tigani-Asil1,*, S.M. EL Sanousi2, M.A. Aljameel3, H. El Beir4, A. Adam5, M.M. Abdallatif6 and M.E. Hamid7.


Cited By:1

Abstract
This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments out of 165 positive AFB specimens, of which 52 isolates were identified as bovine farcy causative agents, while 7 cultures were excluded due to drying. 16S-23S rDNA ITS of NTM revealed three different amplicons 500 bp. (32) isolates, 550 bp. (2) isolates and 600 bp. (14) isolates. Four isolates were contaminated.

Key words: Acid fast bacteria, Bovine farcy, Nontuberculous mycobacteria, Pyogenic infection


 
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How to Cite this Article
Pubmed Style

A.E. El Tigani-Asil,, S.M. EL Sanousi, M.A. Aljameel, H. El Beir, A. Adam, M.M. Abdallatif and M.E. Hamid. Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan. Open Vet J. 2014; 4(1): 16-19. doi:10.5455/OVJ.2014.v4.i1.p16


Web Style

A.E. El Tigani-Asil,, S.M. EL Sanousi, M.A. Aljameel, H. El Beir, A. Adam, M.M. Abdallatif and M.E. Hamid. Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan. https://www.openveterinaryjournal.com/?mno=302656844 [Access: February 13, 2024]. doi:10.5455/OVJ.2014.v4.i1.p16


AMA (American Medical Association) Style

A.E. El Tigani-Asil,, S.M. EL Sanousi, M.A. Aljameel, H. El Beir, A. Adam, M.M. Abdallatif and M.E. Hamid. Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan. Open Vet J. 2014; 4(1): 16-19. doi:10.5455/OVJ.2014.v4.i1.p16



Vancouver/ICMJE Style

A.E. El Tigani-Asil,, S.M. EL Sanousi, M.A. Aljameel, H. El Beir, A. Adam, M.M. Abdallatif and M.E. Hamid. Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan. Open Vet J. (2014), [cited February 13, 2024]; 4(1): 16-19. doi:10.5455/OVJ.2014.v4.i1.p16



Harvard Style

A.E. El Tigani-Asil,, S.M. EL Sanousi, M.A. Aljameel, H. El Beir, A. Adam, M.M. Abdallatif and M.E. Hamid (2014) Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan. Open Vet J, 4 (1), 16-19. doi:10.5455/OVJ.2014.v4.i1.p16



Turabian Style

A.E. El Tigani-Asil,, S.M. EL Sanousi, M.A. Aljameel, H. El Beir, A. Adam, M.M. Abdallatif and M.E. Hamid. 2014. Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan. Open Veterinary Journal, 4 (1), 16-19. doi:10.5455/OVJ.2014.v4.i1.p16



Chicago Style

A.E. El Tigani-Asil,, S.M. EL Sanousi, M.A. Aljameel, H. El Beir, A. Adam, M.M. Abdallatif and M.E. Hamid. "Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan." Open Veterinary Journal 4 (2014), 16-19. doi:10.5455/OVJ.2014.v4.i1.p16



MLA (The Modern Language Association) Style

A.E. El Tigani-Asil,, S.M. EL Sanousi, M.A. Aljameel, H. El Beir, A. Adam, M.M. Abdallatif and M.E. Hamid. "Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan." Open Veterinary Journal 4.1 (2014), 16-19. Print. doi:10.5455/OVJ.2014.v4.i1.p16



APA (American Psychological Association) Style

A.E. El Tigani-Asil,, S.M. EL Sanousi, M.A. Aljameel, H. El Beir, A. Adam, M.M. Abdallatif and M.E. Hamid (2014) Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan. Open Veterinary Journal, 4 (1), 16-19. doi:10.5455/OVJ.2014.v4.i1.p16