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Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa

M.A. Hamedani1,*, A.M. Tahmasbi1 and Y.J. Ahangari2.

Abstract
The present study was undertaken to investigate the effect of various levels of vitamin B12 in Tris extender on semen quality of Dallagh rams following the cooling and freeze/thawing process. Semen was collected from six healthy and mature rams with an average body weight of 60.0 ± 5.0 Kg using an electro ejaculator. High quality samples were mixed and diluted in Tris extender supplemented with different concentrations of vitamin B12 (0, 1, 2 and 3 mg/ml). The semen aliquots were cooled and preserved at 5°C and their qualities were evaluated during pre-freezing and then the cooled semen samples were packaged into 0.25 ml straws. Straws were frozen in the vapor of liquid nitrogen, and were then stored at -196ºC. Straws were thawed seven days later and the characteristics of spermatozoa were examined. Results of this study showed that the effect of vitamin B12 on characteristics such as viability, motility, progressive motility and normality of spermatozoa were significant in pre and post freezing conditions (P<0.05). In conclusion, for long term storage of semen of Dallagh rams, we recommend using 2 mg/ml of vitamin B12 in semen extender.

Key words: Dallagh ram, Freezing, Semen characteristics, Vitamin B12.


 
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Pubmed Style

M.A. Hamedani,, A.M. Tahmasbi and Y.J. Ahangari. Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Vet J. 2013; 3(2): 140-144. doi:10.5455/OVJ.2013.v3.i2.p140


Web Style

M.A. Hamedani,, A.M. Tahmasbi and Y.J. Ahangari. Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. https://www.openveterinaryjournal.com/?mno=302656865 [Access: February 07, 2023]. doi:10.5455/OVJ.2013.v3.i2.p140


AMA (American Medical Association) Style

M.A. Hamedani,, A.M. Tahmasbi and Y.J. Ahangari. Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Vet J. 2013; 3(2): 140-144. doi:10.5455/OVJ.2013.v3.i2.p140



Vancouver/ICMJE Style

M.A. Hamedani,, A.M. Tahmasbi and Y.J. Ahangari. Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Vet J. (2013), [cited February 07, 2023]; 3(2): 140-144. doi:10.5455/OVJ.2013.v3.i2.p140



Harvard Style

M.A. Hamedani,, A.M. Tahmasbi and Y.J. Ahangari (2013) Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Vet J, 3 (2), 140-144. doi:10.5455/OVJ.2013.v3.i2.p140



Turabian Style

M.A. Hamedani,, A.M. Tahmasbi and Y.J. Ahangari. 2013. Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Veterinary Journal, 3 (2), 140-144. doi:10.5455/OVJ.2013.v3.i2.p140



Chicago Style

M.A. Hamedani,, A.M. Tahmasbi and Y.J. Ahangari. "Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa." Open Veterinary Journal 3 (2013), 140-144. doi:10.5455/OVJ.2013.v3.i2.p140



MLA (The Modern Language Association) Style

M.A. Hamedani,, A.M. Tahmasbi and Y.J. Ahangari. "Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa." Open Veterinary Journal 3.2 (2013), 140-144. Print. doi:10.5455/OVJ.2013.v3.i2.p140



APA (American Psychological Association) Style

M.A. Hamedani,, A.M. Tahmasbi and Y.J. Ahangari (2013) Effects of vitamin B12 supplementation on the quality of Ovine spermatozoa. Open Veterinary Journal, 3 (2), 140-144. doi:10.5455/OVJ.2013.v3.i2.p140