E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait

Maha S. AlAzemi, Qais A. H. Majeed, Attia Samy, Adawia A. Henedi, Wessam Youssef, Nadra-Elwgoud M. I. Abdou.

Abstract
Background: Diarrhea in newborn small ruminants continues to be the cause of significant financial losses in sheep and goat farms around the world. Commercial immunochromatographic assays (IC) have been designed and evaluated to be used for the diagnosis of diarrhea in cattle; however, there are no trials to use rapid tests in small ruminants.
Aim: This study was carried out in Kuwait to evaluate the performance of rapid immunochromatography test (BoviD-4, BioNote, Inc, Korea) for diagnostics of Cryptosporidium, rotavirus A (RVA), bovine coronavirus (BCoV) and Escherichia coli K99 (E. coli K99) in fecal samples of small ruminants.
Methods: Eighty-five samples were examined using BoviD- 4, and the results were compared with that of polymerase chain reaction (PCR) for Cryptosporidium, RVA, BCoV, whereas for E. coli K99 by isolation and identification as reference tests.
Results: The Kappa test (κ) agreement results between the rapid assay and reference tests were 0.870 (perfect), 0.783 (substantial), 0.728 (substantial), and 0.281 (fair) for the detection of E. coli K99, Cryptosporidium, RVA, and BCoV, respectively. The sensitivity of BoviD-4 kit was 91.2%, 80.0%, 90.0% and 37.5% and the specificity was 88.2%, 96.0%, 96.4% and 92.2% for Cryptosporidium, RVA, E. coli K99, and BCoV, respectively.
Conclusion: The Bovid-4 kit can be used as a rapid pen-side test for Cryptosporidium spp., E. coli K99, and RVA in the field. Nonetheless, care must be taken while interpreting BCoV results of the kit.

Key words: Diarrhea, Sheep, Goats, Immunochromatography assay, Kuwait.


 
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How to Cite this Article
Pubmed Style

AlAzemi MS, Majeed QAH, Samy A, Henedi AA, Youssef W, NMIA, . Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait. Open Vet J. 2021; 11(3): 500-507. doi:10.5455/OVJ.2021.v11.i3.23


Web Style

AlAzemi MS, Majeed QAH, Samy A, Henedi AA, Youssef W, NMIA, . Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait. https://www.openveterinaryjournal.com/?mno=74188 [Access: February 04, 2023]. doi:10.5455/OVJ.2021.v11.i3.23


AMA (American Medical Association) Style

AlAzemi MS, Majeed QAH, Samy A, Henedi AA, Youssef W, NMIA, . Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait. Open Vet J. 2021; 11(3): 500-507. doi:10.5455/OVJ.2021.v11.i3.23



Vancouver/ICMJE Style

AlAzemi MS, Majeed QAH, Samy A, Henedi AA, Youssef W, NMIA, . Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait. Open Vet J. (2021), [cited February 04, 2023]; 11(3): 500-507. doi:10.5455/OVJ.2021.v11.i3.23



Harvard Style

AlAzemi, M. S., Majeed, Q. A. H., Samy, A., Henedi, A. A., Youssef, W., , N. M. I. A. & (2021) Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait. Open Vet J, 11 (3), 500-507. doi:10.5455/OVJ.2021.v11.i3.23



Turabian Style

AlAzemi, Maha S., Qais A. H. Majeed, Attia Samy, Adawia A. Henedi, Wessam Youssef, Nadra-Elwgoud M. I. Abdou, and . 2021. Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait. Open Veterinary Journal, 11 (3), 500-507. doi:10.5455/OVJ.2021.v11.i3.23



Chicago Style

AlAzemi, Maha S., Qais A. H. Majeed, Attia Samy, Adawia A. Henedi, Wessam Youssef, Nadra-Elwgoud M. I. Abdou, and . "Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait." Open Veterinary Journal 11 (2021), 500-507. doi:10.5455/OVJ.2021.v11.i3.23



MLA (The Modern Language Association) Style

AlAzemi, Maha S., Qais A. H. Majeed, Attia Samy, Adawia A. Henedi, Wessam Youssef, Nadra-Elwgoud M. I. Abdou, and . "Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait." Open Veterinary Journal 11.3 (2021), 500-507. Print. doi:10.5455/OVJ.2021.v11.i3.23



APA (American Psychological Association) Style

AlAzemi, M. S., Majeed, Q. A. H., Samy, A., Henedi, A. A., Youssef, W., , N. M. I. A. & (2021) Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait. Open Veterinary Journal, 11 (3), 500-507. doi:10.5455/OVJ.2021.v11.i3.23