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2024, Vol: 14, Issue: 12
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Research Article


Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae

Najiah M. Alyamani.


Abstract
Background:
Mitochondrial genomes play a key role in molecular biology research by providing essential information about evolutionary links, population history, and genetic diversity.

Aim:
The aim of this investigation was to produce partial sequence of 12S rRNA and 16S rRNA genes, as well as complete sequence of tRNA-Val gene in some species of family Labridae.

Methods:
Five species of labrid fishes (Oxycheilinus digramma, Cheilio inermis, Epibulus insidiator, Coris aygula and Gomphosus caeruleus) belong to Family Labridae were collected from the Red Sea, thereafter, taken to a lab for morphological identification in accordance with Randall (1982). Using forward and reverse primers (Wang, 2000), genome DNA was amplified through polymerase chain reaction.

Results:
The tRNA-Val gene's entire sequence, the 12S rRNA gene's partial sequence, and the 16S rRNA gene's partial sequence were all submitted to GenBank/NCBI with accession numbers (PP962382.1 - PP962386.1). The sequences' outcomes showed that the average A+T values were higher than the C+G values.

Conclusion:
The partial sequences of 12S RNA and 16S RNA, and the whole sequence of the tRNA-Val gene, were arranged that, the 12S RNA and 16S RNA have been distinguished by the tRNA-Val gene.

Key words: Mitochondrial 12S rRNA gene, 16S rRNA gene, tRNA-Val gene, Labridae


 
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How to Cite this Article
Pubmed Style

Najiah M. Alyamani. Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae. Open Vet J. 2024; 14(12): 3336-3344. doi:10.5455/OVJ.2024.v14.i12.18

Web Style

Najiah M. Alyamani. Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae. https://www.openveterinaryjournal.com/?mno=217238 [Access: January 22, 2025]. doi:10.5455/OVJ.2024.v14.i12.18

AMA (American Medical Association) Style

Najiah M. Alyamani. Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae. Open Vet J. 2024; 14(12): 3336-3344. doi:10.5455/OVJ.2024.v14.i12.18


Vancouver/ICMJE Style

Najiah M. Alyamani. Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae. Open Vet J. (2024), [cited January 22, 2025]; 14(12): 3336-3344. doi:10.5455/OVJ.2024.v14.i12.18


Harvard Style

Najiah M. Alyamani (2024) Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae. Open Vet J, 14 (12), 3336-3344. doi:10.5455/OVJ.2024.v14.i12.18


Turabian Style

Najiah M. Alyamani. 2024. Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae. Open Veterinary Journal, 14 (12), 3336-3344. doi:10.5455/OVJ.2024.v14.i12.18


Chicago Style

Najiah M. Alyamani. "Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae." Open Veterinary Journal 14 (2024), 3336-3344. doi:10.5455/OVJ.2024.v14.i12.18


MLA (The Modern Language Association) Style

Najiah M. Alyamani. "Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae." Open Veterinary Journal 14.12 (2024), 3336-3344. Print. doi:10.5455/OVJ.2024.v14.i12.18


APA (American Psychological Association) Style

Najiah M. Alyamani (2024) Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae. Open Veterinary Journal, 14 (12), 3336-3344. doi:10.5455/OVJ.2024.v14.i12.18


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