E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation

Alfi Ruham Burjiah, Ashon Sa’adi, Widjiati Widjiati.

Abstract
Background: Endometriosis is a common benign, estrogen-dependent, and chronic gynecological disease. Immune system disturbances and inflammatory abnormalities were involved in the pathogenesis of endometriosis. , Therefore, it is logical to use vitamin D, which has an immunomodulatory capacity, as supportive therapy for endometriosis.
Aims: This research time to study the effect of different doses of vitamin D on IL-17 expression in endometriosis mice models.
Methods: Endometriosis was induced in twenty-four mice divided into four groups of six. Group C received no treatment, while groups T1, T2, and T3 received graded doses of vitamin D to the stomach, sequentially 8 IU, 16 IU, and 24 IU for three weeks. Interleukin-17 expression and the extent of endometriotic peritoneal lesions were also measured and analyzed. Statistical tests were performed to see the difference in the mean area of endometriosis lesions and IL-17 expression between the control and treatment groups, as well as the correlation between the extent of endometriosis lesions and IL-17. We performed a statistical test to determine whether stratified doses have different IL-17 and endometriosis area outcomes.
Results: The area of endometriosis lesions was decreased after 16 IU and 24 IU of vitamin D administration (p 0.023 and 0.009). Endometriosis lesions also tended to be smaller after 8 IU of vitamin D supplementation, although insignificant (p > 0.05). Interleukin-17 expression was significantly lower after 24 IU vitamin D administration (p = 0.004). Vitamin D doses of 8 IU and 16 IU caused lower IL-17 expression, although not significant (p = 0.452 and p = 0.645). The regulation of IL-17 expression was significantly dependent on the dose level of vitamin D with an optimal dose of 24 IU (p = 0.004). This dose also caused the smallest endometriosis lesion, although not optimal (p > 0.05). IL-17 expression was moderately and positively correlated with the extent of endometriosis lesions (p = 0.012, rho = 0.505).
Conclusion: By modulating the expression of interleukin-17 in endometriotic lesions, vitamin D inhibits the development of endometriotic lesions in the endometriosis mice model. The optimal vitamin D dose in this study was 24 IU.

Key words: Endometriosis lesion size, Interleukin 17, Reproductive Health, Vitamin D


 
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How to Cite this Article
Pubmed Style

Burjiah AR, Sa'adi A, Widjiati W, . Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation. Open Vet J. 2022; 12(6): 956-964. doi:10.5455/OVJ.2022.v12.i6.23


Web Style

Burjiah AR, Sa'adi A, Widjiati W, . Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation. https://www.openveterinaryjournal.com/?mno=115890 [Access: February 04, 2023]. doi:10.5455/OVJ.2022.v12.i6.23


AMA (American Medical Association) Style

Burjiah AR, Sa'adi A, Widjiati W, . Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation. Open Vet J. 2022; 12(6): 956-964. doi:10.5455/OVJ.2022.v12.i6.23



Vancouver/ICMJE Style

Burjiah AR, Sa'adi A, Widjiati W, . Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation. Open Vet J. (2022), [cited February 04, 2023]; 12(6): 956-964. doi:10.5455/OVJ.2022.v12.i6.23



Harvard Style

Burjiah, A. R., Sa'adi, A., Widjiati, W. & (2022) Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation. Open Vet J, 12 (6), 956-964. doi:10.5455/OVJ.2022.v12.i6.23



Turabian Style

Burjiah, Alfi Ruham, Ashon Sa'adi, Widjiati Widjiati, and . 2022. Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation. Open Veterinary Journal, 12 (6), 956-964. doi:10.5455/OVJ.2022.v12.i6.23



Chicago Style

Burjiah, Alfi Ruham, Ashon Sa'adi, Widjiati Widjiati, and . "Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation." Open Veterinary Journal 12 (2022), 956-964. doi:10.5455/OVJ.2022.v12.i6.23



MLA (The Modern Language Association) Style

Burjiah, Alfi Ruham, Ashon Sa'adi, Widjiati Widjiati, and . "Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation." Open Veterinary Journal 12.6 (2022), 956-964. Print. doi:10.5455/OVJ.2022.v12.i6.23



APA (American Psychological Association) Style

Burjiah, A. R., Sa'adi, A., Widjiati, W. & (2022) Vitamin D inhibited endometriosis development in mice model through interleukin 17 modulation. Open Veterinary Journal, 12 (6), 956-964. doi:10.5455/OVJ.2022.v12.i6.23