E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems

María Paula Herrera-Sánchez, Kelly Johanna Lozano-Villegas, Iang Schroniltgen Rondón-Barragán, Roy Rodríguez-Hernández.


Abstract
Background:
The liver and spleen play a pivotal role in metabolism and immune response. During stress, neuroendocrine response induces changes in gene expression and its assessment demands the validation of the stability of the reference genes to perform relative gene expression experiments.
Aim:
The objective of this study was to determine the expression stability of four reference genes (GAPDH, ACTB, RNA18S, and HMBS) in the liver and spleen tissues from laying hens housed in conventional cage (CC) and cage-free (CF) egg production systems.
Methods:
Liver and spleen from Hy-Line Brown hens housed in CC and CF egg production systems were used. mRNA transcript levels were determined by qPCR and the gene expression stability was evaluated using geNorm, BestKeeper and NormFinder algorithms.
Results:
The most stable gene from liver tissue was ACTB in CC, CF and CC-CF group (overall data). In the spleen, the most stables genes were GAPDH (CC), HMBS (CF), and ACTB (CC-CF).
Conclusion:
The ACTB gene was the most stable gene in the liver, and GAPDH and HMBS genes were stable in spleen tissues that could be used for the normalization in qPCR experiments performed in liver and spleen tissues of laying hens housed CC and CF production systems.

Key words: Laying hens, Liver, mRNA, Reference genes, Spleen


 
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How to Cite this Article
Pubmed Style

Herrera-Sánchez MP, Lozano-Villegas KJ, Rondón-Barragán IS, Rodríguez-Hernández R. Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Vet J. 2023; 13(3): 270-277. doi:10.5455/OVJ.2023.v13.i3.3


Web Style

Herrera-Sánchez MP, Lozano-Villegas KJ, Rondón-Barragán IS, Rodríguez-Hernández R. Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. https://www.openveterinaryjournal.com/?mno=133560 [Access: April 09, 2024]. doi:10.5455/OVJ.2023.v13.i3.3


AMA (American Medical Association) Style

Herrera-Sánchez MP, Lozano-Villegas KJ, Rondón-Barragán IS, Rodríguez-Hernández R. Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Vet J. 2023; 13(3): 270-277. doi:10.5455/OVJ.2023.v13.i3.3



Vancouver/ICMJE Style

Herrera-Sánchez MP, Lozano-Villegas KJ, Rondón-Barragán IS, Rodríguez-Hernández R. Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Vet J. (2023), [cited April 09, 2024]; 13(3): 270-277. doi:10.5455/OVJ.2023.v13.i3.3



Harvard Style

Herrera-Sánchez, M. P., Lozano-Villegas, . K. J., Rondón-Barragán, . I. S. & Rodríguez-Hernández, . R. (2023) Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Vet J, 13 (3), 270-277. doi:10.5455/OVJ.2023.v13.i3.3



Turabian Style

Herrera-Sánchez, María Paula, Kelly Johanna Lozano-Villegas, Iang Schroniltgen Rondón-Barragán, and Roy Rodríguez-Hernández. 2023. Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Veterinary Journal, 13 (3), 270-277. doi:10.5455/OVJ.2023.v13.i3.3



Chicago Style

Herrera-Sánchez, María Paula, Kelly Johanna Lozano-Villegas, Iang Schroniltgen Rondón-Barragán, and Roy Rodríguez-Hernández. "Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems." Open Veterinary Journal 13 (2023), 270-277. doi:10.5455/OVJ.2023.v13.i3.3



MLA (The Modern Language Association) Style

Herrera-Sánchez, María Paula, Kelly Johanna Lozano-Villegas, Iang Schroniltgen Rondón-Barragán, and Roy Rodríguez-Hernández. "Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems." Open Veterinary Journal 13.3 (2023), 270-277. Print. doi:10.5455/OVJ.2023.v13.i3.3



APA (American Psychological Association) Style

Herrera-Sánchez, M. P., Lozano-Villegas, . K. J., Rondón-Barragán, . I. S. & Rodríguez-Hernández, . R. (2023) Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Veterinary Journal, 13 (3), 270-277. doi:10.5455/OVJ.2023.v13.i3.3