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Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes

Yktiyar Sarybayev, Yessengali Ussenbekov, Orynbassar Turebekov, Bekbolat Bekbolat, Ibragim Tutkyshbay.


Abstract
Background:
Genotyping offers a promising avenue for identifying the healthy reproductive system in cows. The healthy reproductive system in cows is determined by measuring the level of ovulation and by identifying the type polymorphism of specific genes.
Aim:
The aim of the article is to explore how polymorphism of Follicle Stimulating Hormone Receptor (FSHR) and Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) genes affect the reproduction trait of Holstein cows.
Methods:
Here we define a reproducible protocol to genotype and identify the polymorphism in specific genes from the extracted DNA of cows.
Results:
The results of genotyping showed that the only C allele (CC genotype) was observed in 100% of cows at the LHCGR locus, and three genotypes were observed at the FSHR locus (CC – 67.74%, CG - 9.03%, GG – 23.22%). In cows with the CC genotype at the FSHR locus, the hormone concentration during ovulation was 1.1 – 2.5 ng/ml, which is within the physiological range for healthy reproduction.
Conclusion:
Cows with the CC genotype at the FSHR locus have a healthy course of the ovulation process, therefore good reproduction.

Key words: Genotyping, Ovulation, LHCGR, FSHR, Polymorphism


 
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How to Cite this Article
Pubmed Style

Sarybayev Y, Ussenbekov Y, Turebekov O, BB, Tutkyshbay I. Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes. Open Vet J. 2023; 13(3): 352-357. doi:10.5455/OVJ.2023.v13.i3.12

Web Style

Sarybayev Y, Ussenbekov Y, Turebekov O, BB, Tutkyshbay I. Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes. https://www.openveterinaryjournal.com/?mno=136165 [Access: April 21, 2024]. doi:10.5455/OVJ.2023.v13.i3.12

AMA (American Medical Association) Style

Sarybayev Y, Ussenbekov Y, Turebekov O, BB, Tutkyshbay I. Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes. Open Vet J. 2023; 13(3): 352-357. doi:10.5455/OVJ.2023.v13.i3.12


Vancouver/ICMJE Style

Sarybayev Y, Ussenbekov Y, Turebekov O, BB, Tutkyshbay I. Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes. Open Vet J. (2023), [cited April 21, 2024]; 13(3): 352-357. doi:10.5455/OVJ.2023.v13.i3.12


Harvard Style

Sarybayev, Y., Ussenbekov, . Y., Turebekov, . O., , . B. B. & Tutkyshbay, . I. (2023) Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes. Open Vet J, 13 (3), 352-357. doi:10.5455/OVJ.2023.v13.i3.12


Turabian Style

Sarybayev, Yktiyar, Yessengali Ussenbekov, Orynbassar Turebekov, Bekbolat Bekbolat, and Ibragim Tutkyshbay. 2023. Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes. Open Veterinary Journal, 13 (3), 352-357. doi:10.5455/OVJ.2023.v13.i3.12


Chicago Style

Sarybayev, Yktiyar, Yessengali Ussenbekov, Orynbassar Turebekov, Bekbolat Bekbolat, and Ibragim Tutkyshbay. "Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes." Open Veterinary Journal 13 (2023), 352-357. doi:10.5455/OVJ.2023.v13.i3.12


MLA (The Modern Language Association) Style

Sarybayev, Yktiyar, Yessengali Ussenbekov, Orynbassar Turebekov, Bekbolat Bekbolat, and Ibragim Tutkyshbay. "Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes." Open Veterinary Journal 13.3 (2023), 352-357. Print. doi:10.5455/OVJ.2023.v13.i3.12


APA (American Psychological Association) Style

Sarybayev, Y., Ussenbekov, . Y., Turebekov, . O., , . B. B. & Tutkyshbay, . I. (2023) Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes. Open Veterinary Journal, 13 (3), 352-357. doi:10.5455/OVJ.2023.v13.i3.12


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