E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula

Aboubaker M. Garbaj, Samira A. Farag, Jihan A. Sherif, Aml F. Lawila, Hanan L. Eshamah, Salah M. Azwai, Fatim T. Gammoudi, Hesham T. Naas, Allaaeddin A. El-Salabi, Ibrahim M. Eldaghayes.


Abstract
Background:
Cronobacter species is the most significant foodborne pathogens in infant milk formula (IMF). These pathogens have been incriminated in sever form of neonatal meningitis, sepsis and necrotizing enterocolitis with high mortality rate.
Aim:
The current study was performed to demonstrate the effect of heat stress on Cronobacter spp. (C. sakazakii and C. pulveris) in reconstituted IMF (RIMF).
Methods:
The reconstituted formula was inoculated with five C. sakazakii isolates and four C. pulveris isolates separately. The nine isolates of Cronobacter spp. were heated in RIMF at 48, 52, 56, 60, 64 and 66°C. The D- and z-values were determined by using linear regression analysis.
Results:
The D˗values of all isolates of C. sakazakii (CS1, CS3, CS4, CS5 and CS6) at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C ranged from 7.29 ˗ 23.47, 2.77˗15.50, 0.62˗1.04, 0.62˗1.02, 0.62˗1.00, 0.62˗1.00 min, respectively, while the z˗ values extended from 2.50˗4.28°C. The D˗ values of C. pulveris isolates (CP1, CP2, CP3, CP4) ranged from 7.60 ˗ 22.32, 1.42 ˗ 8.45, 0.62 ˗ 1.08, 0.62 ˗ 0.78, 0.62 ˗ 0.78, 0.62 ˗ 0.79 min. at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C, respectively and the calculated z-values ranged from 3.33 ˗ 4.89°C.
Conclusion:
This study may contribute to improve the understanding of behavior of C. sakazakii and C. pulveris isolates in RIMF at various heat stress temperature and may participate in effective control of this pathogens in infant’s foods production.

Key words: Cronobacter sakazakii, Cronobacter pulveris, Thermo-tolerance, D˗ value, z˗ value, Infant milk formula


 
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How to Cite this Article
Pubmed Style

Garbaj AM, Farag SA, Sherif JA, Lawila AF, Eshamah HL, Azwai SM, Gammoudi FT, Naas HT, El-Salabi AA, Eldaghayes IM. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Vet J. 2023; 13(1): 108-113. doi:10.5455/OVJ.2023.v13.i1.11


Web Style

Garbaj AM, Farag SA, Sherif JA, Lawila AF, Eshamah HL, Azwai SM, Gammoudi FT, Naas HT, El-Salabi AA, Eldaghayes IM. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. https://www.openveterinaryjournal.com/?mno=138198 [Access: February 13, 2024]. doi:10.5455/OVJ.2023.v13.i1.11


AMA (American Medical Association) Style

Garbaj AM, Farag SA, Sherif JA, Lawila AF, Eshamah HL, Azwai SM, Gammoudi FT, Naas HT, El-Salabi AA, Eldaghayes IM. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Vet J. 2023; 13(1): 108-113. doi:10.5455/OVJ.2023.v13.i1.11



Vancouver/ICMJE Style

Garbaj AM, Farag SA, Sherif JA, Lawila AF, Eshamah HL, Azwai SM, Gammoudi FT, Naas HT, El-Salabi AA, Eldaghayes IM. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Vet J. (2023), [cited February 13, 2024]; 13(1): 108-113. doi:10.5455/OVJ.2023.v13.i1.11



Harvard Style

Garbaj, A. M., Farag, . S. A., Sherif, . J. A., Lawila, . A. F., Eshamah, . H. L., Azwai, . S. M., Gammoudi, . F. T., Naas, . H. T., El-Salabi, . A. A. & Eldaghayes, . I. M. (2023) Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Vet J, 13 (1), 108-113. doi:10.5455/OVJ.2023.v13.i1.11



Turabian Style

Garbaj, Aboubaker M., Samira A. Farag, Jihan A. Sherif, Aml F. Lawila, Hanan L. Eshamah, Salah M. Azwai, Fatim T. Gammoudi, Hesham T. Naas, Allaaeddin A. El-Salabi, and Ibrahim M. Eldaghayes. 2023. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Veterinary Journal, 13 (1), 108-113. doi:10.5455/OVJ.2023.v13.i1.11



Chicago Style

Garbaj, Aboubaker M., Samira A. Farag, Jihan A. Sherif, Aml F. Lawila, Hanan L. Eshamah, Salah M. Azwai, Fatim T. Gammoudi, Hesham T. Naas, Allaaeddin A. El-Salabi, and Ibrahim M. Eldaghayes. "Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula." Open Veterinary Journal 13 (2023), 108-113. doi:10.5455/OVJ.2023.v13.i1.11



MLA (The Modern Language Association) Style

Garbaj, Aboubaker M., Samira A. Farag, Jihan A. Sherif, Aml F. Lawila, Hanan L. Eshamah, Salah M. Azwai, Fatim T. Gammoudi, Hesham T. Naas, Allaaeddin A. El-Salabi, and Ibrahim M. Eldaghayes. "Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula." Open Veterinary Journal 13.1 (2023), 108-113. Print. doi:10.5455/OVJ.2023.v13.i1.11



APA (American Psychological Association) Style

Garbaj, A. M., Farag, . S. A., Sherif, . J. A., Lawila, . A. F., Eshamah, . H. L., Azwai, . S. M., Gammoudi, . F. T., Naas, . H. T., El-Salabi, . A. A. & Eldaghayes, . I. M. (2023) Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Veterinary Journal, 13 (1), 108-113. doi:10.5455/OVJ.2023.v13.i1.11