E-ISSN 2218-6050 | ISSN 2226-4485
 

Research Article


Asiatic acid in Centella asiatica extract towards morphological development in an intermittent hypoxia intrauterine embryo model and molecular prediction pathway of insulin-like growth factor-1 (IGF-1) receptor signalling

Ariani Ariani, Imelda Febriana Ghofar, Husnul Khotimah, Nurdiana Nurdiana, Masruroh Rahayu.


Abstract
Background:
Hypoxia during pregnancy generates oxidative stress that alters the growth and development of the human fetus. Insulin-like growth factor-I (IGF-1) receptors are essential for normal fetal growth. Asiatic acid in Centella asiatica (CA) has antioxidant properties to prevent growth impairment in hypoxia.
Aims:
This study aimed to investigate the effect of asiatic acid on the morphological development of an intermittent hypoxia zebrafish embryo model and analyse molecular docking prediction in insulin-like growth factor-1 receptor (IGF-1R) signalling.
Methods:
Embryos of zebrafish at 2 hours postfertilization (hpf) were assigned to control negative (C), intermittent hypoxia (IH), and combination IH and CA extract groups consisting of 1.25 (IHCA1), 2.5 (IHCA2), and 5 (IHCA3) µg/ml. Hypoxia treatment (conducted 4 hours/day) and CA extract were administered for 3 days (2 - 72 hpf). The parameters of body length and head length were evaluated at 3, 6, and 9 days postfertilization (dpf). The data were analysed by a two-way ANOVA (P<0.05). Molecular docking was performed to explore the binding affinity of asiatic acid to IGF-1R by Molegro Virtual Docker ver.5 software.
Results:
The body length and head length of embryos in the IH and treatment groups (IHCA) were shorter than those in the control group at 3 dpf (p<0.05). However, the body length was more prolonged in the IHCA1 group, but the head length was longer in the IHCA2 group than in the IH group at 6 and 9 dpf. Molecular docking showed the reliable interaction of asiatic acid with IGF-1R signalling in an IH animal model.
Conclusion:
The administration of CA extract benefits intermittent hypoxia through the development and growth of zebrafish embryos at a dose of 2.5 to 5 µg/ml. Asiatic acid has a binding affinity for IGF-1R signalling.

Key words: Asiatic acid, Centella asiatica, Zebrafish embryo, Morphology, IGF-1R


 
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How to Cite this Article
Pubmed Style

Ariani A, Ghofar IF, Khotimah H, Nurdiana N, Rahayu M. Asiatic acid in Centella asiatica extract towards morphological development in an intermittent hypoxia intrauterine embryo model and molecular prediction pathway of insulin-like growth factor-1 (IGF-1) receptor signalling. Open Vet J. 2023; 13(5): 629-637. doi:10.5455/OVJ.2023.v13.i5.16


Web Style

Ariani A, Ghofar IF, Khotimah H, Nurdiana N, Rahayu M. Asiatic acid in Centella asiatica extract towards morphological development in an intermittent hypoxia intrauterine embryo model and molecular prediction pathway of insulin-like growth factor-1 (IGF-1) receptor signalling. https://www.openveterinaryjournal.com/?mno=142889 [Access: February 13, 2024]. doi:10.5455/OVJ.2023.v13.i5.16


AMA (American Medical Association) Style

Ariani A, Ghofar IF, Khotimah H, Nurdiana N, Rahayu M. Asiatic acid in Centella asiatica extract towards morphological development in an intermittent hypoxia intrauterine embryo model and molecular prediction pathway of insulin-like growth factor-1 (IGF-1) receptor signalling. Open Vet J. 2023; 13(5): 629-637. doi:10.5455/OVJ.2023.v13.i5.16



Vancouver/ICMJE Style

Ariani A, Ghofar IF, Khotimah H, Nurdiana N, Rahayu M. Asiatic acid in Centella asiatica extract towards morphological development in an intermittent hypoxia intrauterine embryo model and molecular prediction pathway of insulin-like growth factor-1 (IGF-1) receptor signalling. Open Vet J. (2023), [cited February 13, 2024]; 13(5): 629-637. doi:10.5455/OVJ.2023.v13.i5.16



Harvard Style

Ariani, A., Ghofar, . I. F., Khotimah, . H., Nurdiana, . N. & Rahayu, . M. (2023) Asiatic acid in Centella asiatica extract towards morphological development in an intermittent hypoxia intrauterine embryo model and molecular prediction pathway of insulin-like growth factor-1 (IGF-1) receptor signalling. Open Vet J, 13 (5), 629-637. doi:10.5455/OVJ.2023.v13.i5.16



Turabian Style

Ariani, Ariani, Imelda Febriana Ghofar, Husnul Khotimah, Nurdiana Nurdiana, and Masruroh Rahayu. 2023. Asiatic acid in Centella asiatica extract towards morphological development in an intermittent hypoxia intrauterine embryo model and molecular prediction pathway of insulin-like growth factor-1 (IGF-1) receptor signalling. Open Veterinary Journal, 13 (5), 629-637. doi:10.5455/OVJ.2023.v13.i5.16



Chicago Style

Ariani, Ariani, Imelda Febriana Ghofar, Husnul Khotimah, Nurdiana Nurdiana, and Masruroh Rahayu. "Asiatic acid in Centella asiatica extract towards morphological development in an intermittent hypoxia intrauterine embryo model and molecular prediction pathway of insulin-like growth factor-1 (IGF-1) receptor signalling." Open Veterinary Journal 13 (2023), 629-637. doi:10.5455/OVJ.2023.v13.i5.16



MLA (The Modern Language Association) Style

Ariani, Ariani, Imelda Febriana Ghofar, Husnul Khotimah, Nurdiana Nurdiana, and Masruroh Rahayu. "Asiatic acid in Centella asiatica extract towards morphological development in an intermittent hypoxia intrauterine embryo model and molecular prediction pathway of insulin-like growth factor-1 (IGF-1) receptor signalling." Open Veterinary Journal 13.5 (2023), 629-637. Print. doi:10.5455/OVJ.2023.v13.i5.16



APA (American Psychological Association) Style

Ariani, A., Ghofar, . I. F., Khotimah, . H., Nurdiana, . N. & Rahayu, . M. (2023) Asiatic acid in Centella asiatica extract towards morphological development in an intermittent hypoxia intrauterine embryo model and molecular prediction pathway of insulin-like growth factor-1 (IGF-1) receptor signalling. Open Veterinary Journal, 13 (5), 629-637. doi:10.5455/OVJ.2023.v13.i5.16