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Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Isolated From Diarrheic Calves in Argentina

Ramon Alejandro Gonzalez Pasayo, Marcelo Eduardo Sanz, Nora Lia Padola, Ana Rita Moreira.


Cited By:13

Abstract
Enterotoxigenic Escherichia coli (ETEC) is the most common and global cause of neonatal calf diarrhea, but there is little information regarding calf ETEC strains in Argentina. In this study, five ETEC isolates from diarrheic dairy calves (2-10 days-old) from Buenos Aires and Cordoba, Argentina, were characterized on the basis of virulence gene (VG) pattern, O:H serotyping, hemolytic phenotype, phylogenetic group affiliation, antimicrobial resistance profile and presence of integron class 1 and 2.
The five isolates were examined by PCR for the presence of 18 bovine VGs and showed the following genotypes: F5+/F41+/sta+ (D242), F5+/sta+ (D158), F5+/sta+ (D157), F5+ (D151-9) and F5+/iucD+ (D151-5). These VGs confer pathogenic potential and most of them are associated with the ETEC pathotype. The five isolates showed a non-hemolytic phenotype, belonged to five different serotypes: O101:H-, O141:H-, O60:H-, ONT:H10, ONT:H- and were assigned to the phylogenetic group A by the quadruplex Clermont PCR method. The antimicrobial (AM) resistance of the three isolates D242, D157 and D151-5 was determined by agar disk diffusion method for 24 AMs and they exhibited a multiresistance phenotype (resistance to 4 different AM classes: Cephalosporins, Penicillins, Macrolides and Ansamycins). In addition, class 1 integrons were found in the isolate D151-5 containing the dfrA17-aadA5 gene cassette and in the bovine ETEC reference strain FV10191 containing the dfrA1-aadA1 gene cassette. The present study revealed for the first time the occurrence of multiresistant ETEC associated with neonatal diarrhea in dairy calves in Argentina. This finding may be used for diagnostic and therapeutic purposes.

Key words: Escherichia coli, dairy cattle, neonatal diarrhea, virulence gene, antimicrobial resistance.


 
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How to Cite this Article
Pubmed Style

Pasayo RAG, Sanz ME, Padola NL, Moreira AR, . Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Isolated From Diarrheic Calves in Argentina. Open Vet J. 2019; 9(1): 65-73. doi:10.4314/ovj.v9i1.12


Web Style

Pasayo RAG, Sanz ME, Padola NL, Moreira AR, . Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Isolated From Diarrheic Calves in Argentina. https://www.openveterinaryjournal.com/?mno=1794 [Access: February 28, 2024]. doi:10.4314/ovj.v9i1.12


AMA (American Medical Association) Style

Pasayo RAG, Sanz ME, Padola NL, Moreira AR, . Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Isolated From Diarrheic Calves in Argentina. Open Vet J. 2019; 9(1): 65-73. doi:10.4314/ovj.v9i1.12



Vancouver/ICMJE Style

Pasayo RAG, Sanz ME, Padola NL, Moreira AR, . Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Isolated From Diarrheic Calves in Argentina. Open Vet J. (2019), [cited February 28, 2024]; 9(1): 65-73. doi:10.4314/ovj.v9i1.12



Harvard Style

Pasayo, R. A. G., Sanz, M. E., Padola, N. . L., Moreira, A. . R. & (2019) Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Isolated From Diarrheic Calves in Argentina. Open Vet J, 9 (1), 65-73. doi:10.4314/ovj.v9i1.12



Turabian Style

Pasayo, Ramon Alejandro Gonzalez, Marcelo Eduardo Sanz, Nora Lia Padola, Ana Rita Moreira, and . 2019. Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Isolated From Diarrheic Calves in Argentina. Open Veterinary Journal, 9 (1), 65-73. doi:10.4314/ovj.v9i1.12



Chicago Style

Pasayo, Ramon Alejandro Gonzalez, Marcelo Eduardo Sanz, Nora Lia Padola, Ana Rita Moreira, and . "Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Isolated From Diarrheic Calves in Argentina." Open Veterinary Journal 9 (2019), 65-73. doi:10.4314/ovj.v9i1.12



MLA (The Modern Language Association) Style

Pasayo, Ramon Alejandro Gonzalez, Marcelo Eduardo Sanz, Nora Lia Padola, Ana Rita Moreira, and . "Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Isolated From Diarrheic Calves in Argentina." Open Veterinary Journal 9.1 (2019), 65-73. Print. doi:10.4314/ovj.v9i1.12



APA (American Psychological Association) Style

Pasayo, R. A. G., Sanz, M. E., Padola, N. . L., Moreira, A. . R. & (2019) Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia coli Isolated From Diarrheic Calves in Argentina. Open Veterinary Journal, 9 (1), 65-73. doi:10.4314/ovj.v9i1.12