E-ISSN 2218-6050 | ISSN 2226-4485
 

Research Article


Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus)

José Goicochea-Vargas, Max Salvatierra-Alor, Fidel Acosta-Pachorro, Wilson Rondón-Jorge, Arnold Herrera-Briceño, Edson Morales-Parra, Eric Mialhe.


Abstract
Background:
Presently, there exists a growing interest in mitigating the utilization of antibiotics in response to the challenges emanating from their usage in livestock. A viable alternative strategy encompasses the introduction of live microorganisms recognized as probiotics, exerting advantageous impacts on the immune system and nutritional aspects of the host animals. Native lactic acid bacteria, inherently possessing specific properties and adaptive capabilities tailored to each animal, are deemed optimal contenders for probiotic advancement.
Aim:
In the current investigation, microorganisms exhibiting probiotic potential were isolated, characterized, and identified from the fecal samples of guinea pigs (Cavia porcellus) belonging to the Peruvian breed.
Methods:
The lactic acid bacteria isolated on MRS agar underwent Gram staining, catalase testing, proteolytic, amylolytic, and cellulolytic activity assays, low pH tolerance assessment, hemolytic evaluation, antagonism against Salmonella sp., determination of autoaggregation and coaggregation capacity, and genotypic characterization through sequencing of the 16S rRNA gene.
Results:
A total of 33 lactic acid bacteria were isolated from the feces of 30 guinea pigs, with 10 selected based on Gram staining and catalase testing. All strains exhibited proteolytic activity, although only one demonstrated amylolytic capability, while none displayed cellulase activity. These bacteria demonstrated higher tolerance to pH 5.0 and, to a lesser extent, to pH 4.0. Furthermore, they exhibited antagonistic activity against Salmonella sp. Only 2 bacteria demonstrated hemolytic activity, and they were subsequently excluded from further evaluations. Subsequent assessments revealed autoaggregation capacities ranging from 4.55% to 23.19%, with a lesser degree of coaggregation with Salmonella sp. ranging from 3.53% to 8.94% for the remaining 8 bacterial isolates. Based on these comprehensive tests, 5 bacteria with notable probiotic potential were identified through molecular assays, belonging to the groups Leuconostoc citreum, Enterococcus gallinarum, Exiguobacterium sp., and Lactococcus lactis.
Conclusion:
The identified bacteria stand out as promising probiotic candidates, deserving further assessment in Peruvian breed guinea pigs. This exploration aims to enhance production outcomes while mitigating the adverse effects induced by pathogenic microorganisms.

Key words: Lactic acid bacteria, Enzymatic capacity, Salmonella sp., PCR, 16S rRNA.


 
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How to Cite this Article
Pubmed Style

Goicochea-Vargas J, Salvatierra-Alor M, Acosta-Pachorro F, Rondón-Jorge W, Herrera-Briceño A, Morales-Parra E, Mialhe E. Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus). Open Vet J. 2024; 14(2): 716-729. doi:10.5455/OVJ.2024.v14.i2.12


Web Style

Goicochea-Vargas J, Salvatierra-Alor M, Acosta-Pachorro F, Rondón-Jorge W, Herrera-Briceño A, Morales-Parra E, Mialhe E. Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus). https://www.openveterinaryjournal.com/?mno=180402 [Access: April 09, 2024]. doi:10.5455/OVJ.2024.v14.i2.12


AMA (American Medical Association) Style

Goicochea-Vargas J, Salvatierra-Alor M, Acosta-Pachorro F, Rondón-Jorge W, Herrera-Briceño A, Morales-Parra E, Mialhe E. Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus). Open Vet J. 2024; 14(2): 716-729. doi:10.5455/OVJ.2024.v14.i2.12



Vancouver/ICMJE Style

Goicochea-Vargas J, Salvatierra-Alor M, Acosta-Pachorro F, Rondón-Jorge W, Herrera-Briceño A, Morales-Parra E, Mialhe E. Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus). Open Vet J. (2024), [cited April 09, 2024]; 14(2): 716-729. doi:10.5455/OVJ.2024.v14.i2.12



Harvard Style

Goicochea-Vargas, J., Salvatierra-Alor, . M., Acosta-Pachorro, . F., Rondón-Jorge, . W., Herrera-Briceño, . A., Morales-Parra, . E. & Mialhe, . E. (2024) Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus). Open Vet J, 14 (2), 716-729. doi:10.5455/OVJ.2024.v14.i2.12



Turabian Style

Goicochea-Vargas, José, Max Salvatierra-Alor, Fidel Acosta-Pachorro, Wilson Rondón-Jorge, Arnold Herrera-Briceño, Edson Morales-Parra, and Eric Mialhe. 2024. Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus). Open Veterinary Journal, 14 (2), 716-729. doi:10.5455/OVJ.2024.v14.i2.12



Chicago Style

Goicochea-Vargas, José, Max Salvatierra-Alor, Fidel Acosta-Pachorro, Wilson Rondón-Jorge, Arnold Herrera-Briceño, Edson Morales-Parra, and Eric Mialhe. "Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus)." Open Veterinary Journal 14 (2024), 716-729. doi:10.5455/OVJ.2024.v14.i2.12



MLA (The Modern Language Association) Style

Goicochea-Vargas, José, Max Salvatierra-Alor, Fidel Acosta-Pachorro, Wilson Rondón-Jorge, Arnold Herrera-Briceño, Edson Morales-Parra, and Eric Mialhe. "Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus)." Open Veterinary Journal 14.2 (2024), 716-729. Print. doi:10.5455/OVJ.2024.v14.i2.12



APA (American Psychological Association) Style

Goicochea-Vargas, J., Salvatierra-Alor, . M., Acosta-Pachorro, . F., Rondón-Jorge, . W., Herrera-Briceño, . A., Morales-Parra, . E. & Mialhe, . E. (2024) Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus). Open Veterinary Journal, 14 (2), 716-729. doi:10.5455/OVJ.2024.v14.i2.12