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Research Article


Clinacanthus nutans leaf extract reduces pancreatic β-cell apoptosis by inhibiting JNK activation and modulating oxidative stress and inflammation in Streptozotocin-induced diabetic rats

Nurlaili Susanti, Arifa Mustika, Junaidi Khotib.


Abstract
Background:
Controlling apoptosis induced by oxidative stress in pancreatic β-cells provides promising strategies for preventing and treating diabetes. Clinacanthus nutans leaves possess bioactive constituents with potential antioxidant and anti-diabetic properties.
Aim:
This study aimed to investigate the molecular mechanisms by which C. nutans extract protects pancreatic β-cells from apoptotic damage in streptozotocin-induced diabetic rats.
Methods:
Diabetes was induced in male Wistar rats by intraperitoneal injection of 45 mg/kg streptozotocin, followed by 28 days of treatment with C. nutans leaf extract and Glibenclamide as the standard drug. At the end of the study, blood samples were collected to measure glucose levels, oxidative stress markers, and inflammation. Pancreatic tissue was stained immunohistochemically to detect c-Jun N-terminal kinase (JNK) and Caspase-3 expression.
Results:
The administration of C. nutans leaf extract to diabetic rats significantly reduced fasting blood glucose, malondialdehyde, and tumor necrosis factor-α levels, while concurrently enhancing the activity of superoxide dismutase. The immunohistochemical studies revealed a decrease in the expression of JNK and caspase-3 in the pancreatic islets of diabetic rats.
Conclusion:
C. nutans exhibits the potential to protect pancreatic β-cells from apoptosis by suppressing oxidative stress and inflammation.

Key words: Apoptosis, Clinacanthus nutans, Diabetes, Inflammation, Oxidative stress


 
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How to Cite this Article
Pubmed Style

NS, AM, Khotib J. Clinacanthus nutans leaf extract reduces pancreatic β-cell apoptosis by inhibiting JNK activation and modulating oxidative stress and inflammation in Streptozotocin-induced diabetic rats. Open Vet J. 2024; 14(2): 730-737. doi:10.5455/OVJ.2024.v14.i2.13

Web Style

NS, AM, Khotib J. Clinacanthus nutans leaf extract reduces pancreatic β-cell apoptosis by inhibiting JNK activation and modulating oxidative stress and inflammation in Streptozotocin-induced diabetic rats. https://www.openveterinaryjournal.com/?mno=174651 [Access: November 08, 2024]. doi:10.5455/OVJ.2024.v14.i2.13

AMA (American Medical Association) Style

NS, AM, Khotib J. Clinacanthus nutans leaf extract reduces pancreatic β-cell apoptosis by inhibiting JNK activation and modulating oxidative stress and inflammation in Streptozotocin-induced diabetic rats. Open Vet J. 2024; 14(2): 730-737. doi:10.5455/OVJ.2024.v14.i2.13


Vancouver/ICMJE Style

NS, AM, Khotib J. Clinacanthus nutans leaf extract reduces pancreatic β-cell apoptosis by inhibiting JNK activation and modulating oxidative stress and inflammation in Streptozotocin-induced diabetic rats. Open Vet J. (2024), [cited November 08, 2024]; 14(2): 730-737. doi:10.5455/OVJ.2024.v14.i2.13


Harvard Style

, N. S., , . A. M. & Khotib, . J. (2024) Clinacanthus nutans leaf extract reduces pancreatic β-cell apoptosis by inhibiting JNK activation and modulating oxidative stress and inflammation in Streptozotocin-induced diabetic rats. Open Vet J, 14 (2), 730-737. doi:10.5455/OVJ.2024.v14.i2.13


Turabian Style

, Nurlaili Susanti, Arifa Mustika, and Junaidi Khotib. 2024. Clinacanthus nutans leaf extract reduces pancreatic β-cell apoptosis by inhibiting JNK activation and modulating oxidative stress and inflammation in Streptozotocin-induced diabetic rats. Open Veterinary Journal, 14 (2), 730-737. doi:10.5455/OVJ.2024.v14.i2.13


Chicago Style

, Nurlaili Susanti, Arifa Mustika, and Junaidi Khotib. "Clinacanthus nutans leaf extract reduces pancreatic β-cell apoptosis by inhibiting JNK activation and modulating oxidative stress and inflammation in Streptozotocin-induced diabetic rats." Open Veterinary Journal 14 (2024), 730-737. doi:10.5455/OVJ.2024.v14.i2.13


MLA (The Modern Language Association) Style

, Nurlaili Susanti, Arifa Mustika, and Junaidi Khotib. "Clinacanthus nutans leaf extract reduces pancreatic β-cell apoptosis by inhibiting JNK activation and modulating oxidative stress and inflammation in Streptozotocin-induced diabetic rats." Open Veterinary Journal 14.2 (2024), 730-737. Print. doi:10.5455/OVJ.2024.v14.i2.13


APA (American Psychological Association) Style

, N. S., , . A. M. & Khotib, . J. (2024) Clinacanthus nutans leaf extract reduces pancreatic β-cell apoptosis by inhibiting JNK activation and modulating oxidative stress and inflammation in Streptozotocin-induced diabetic rats. Open Veterinary Journal, 14 (2), 730-737. doi:10.5455/OVJ.2024.v14.i2.13


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