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Research Article


Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells

Ahmed Abdelfattah-Hassan, Doaa Ibrahim, Ayman A. Saleh, Asmaa T.Y. Kishawy, Reham H.A. Mohamed, Safaa I. Khater.


Abstract
Background:
5-FU is an antimetabolic agent used for treating slowly growing solid tumors like breast and ovarian carcinoma. Thymoquinone (TQ) is a main biologically active constituent of Nigella sativa, it has been found to demonstrate anticancerous effects in several preclinical studies, and this is because TQ possesses multi-target nature. Stem cells-derived exosomes are in the spotlight of research and are promising tissue regenerative and anticancer cell-derived nanovesicles.
Aim:
Herein, we studied the antineoplastic effects of Exosomes derived from mammary stem cells (MaSCs-Exo) on breast cancer cells, alone or combined with TQ when compared to a breast cancer chemotherapeutic agent; 5-FU.
Methods:
Our approach included performing viability test and measuring the expression of pro-apoptotic gene (Bax), anti-apoptotic gene (BCL-2) and angiogenic gene (VEGF) on Human MCF-7 cells (breast adenocarcinoma cells), the MCF-7 cells were cultured and incubated with medium containing 5-FU (25 μg/mL), Thymoquinone (200 μg/mL), MaSCs-Exo (100 μg protein equivalent), a combination of TQ (200 μg/mL) and MaSCs-Exo (100 μg).
Results:
Our obtained results show that TQ and MaSCs-Exo each can effectively inhibit breast cancer cell line (MCF-7) proliferation and growth. Also, the results show that the combination of TQ and MaSCs-Exo had higher cytotoxic effects on MCF-7 breast cancer cells than TQ or 5-FU, alone.
Conclusion:
The present study shows promising anticancer potential of exosomes isolated from mammary stem cells; this effect was potentiated by adding thymoquinone with MaSCs-derived exosomes.

Key words: Mammary stem cells, 5-fluorouracil, Apoptosis, Breast cancer, Anti-tumor


 
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How to Cite this Article
Pubmed Style

Abdelfattah-Hassan A, Ibrahim D, Saleh AA, Kishawy AT, Mohamed RH, Khater SI. Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Vet J. 2024; 14((1) (Zagazig Veterinary Conference)): 525-533. doi:10.5455/OVJ.2024.v14.i1.47


Web Style

Abdelfattah-Hassan A, Ibrahim D, Saleh AA, Kishawy AT, Mohamed RH, Khater SI. Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. https://www.openveterinaryjournal.com/?mno=182418 [Access: April 09, 2024]. doi:10.5455/OVJ.2024.v14.i1.47


AMA (American Medical Association) Style

Abdelfattah-Hassan A, Ibrahim D, Saleh AA, Kishawy AT, Mohamed RH, Khater SI. Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Vet J. 2024; 14((1) (Zagazig Veterinary Conference)): 525-533. doi:10.5455/OVJ.2024.v14.i1.47



Vancouver/ICMJE Style

Abdelfattah-Hassan A, Ibrahim D, Saleh AA, Kishawy AT, Mohamed RH, Khater SI. Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Vet J. (2024), [cited April 09, 2024]; 14((1) (Zagazig Veterinary Conference)): 525-533. doi:10.5455/OVJ.2024.v14.i1.47



Harvard Style

Abdelfattah-Hassan, A., Ibrahim, . D., Saleh, . A. A., Kishawy, . A. T., Mohamed, . R. H. & Khater, . S. I. (2024) Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Vet J, 14 ((1) (Zagazig Veterinary Conference)), 525-533. doi:10.5455/OVJ.2024.v14.i1.47



Turabian Style

Abdelfattah-Hassan, Ahmed, Doaa Ibrahim, Ayman A. Saleh, Asmaa T.Y. Kishawy, Reham H.A. Mohamed, and Safaa I. Khater. 2024. Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Veterinary Journal, 14 ((1) (Zagazig Veterinary Conference)), 525-533. doi:10.5455/OVJ.2024.v14.i1.47



Chicago Style

Abdelfattah-Hassan, Ahmed, Doaa Ibrahim, Ayman A. Saleh, Asmaa T.Y. Kishawy, Reham H.A. Mohamed, and Safaa I. Khater. "Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells." Open Veterinary Journal 14 (2024), 525-533. doi:10.5455/OVJ.2024.v14.i1.47



MLA (The Modern Language Association) Style

Abdelfattah-Hassan, Ahmed, Doaa Ibrahim, Ayman A. Saleh, Asmaa T.Y. Kishawy, Reham H.A. Mohamed, and Safaa I. Khater. "Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells." Open Veterinary Journal 14.(1) (Zagazig Veterinary Conference) (2024), 525-533. Print. doi:10.5455/OVJ.2024.v14.i1.47



APA (American Psychological Association) Style

Abdelfattah-Hassan, A., Ibrahim, . D., Saleh, . A. A., Kishawy, . A. T., Mohamed, . R. H. & Khater, . S. I. (2024) Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Veterinary Journal, 14 ((1) (Zagazig Veterinary Conference)), 525-533. doi:10.5455/OVJ.2024.v14.i1.47