E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 10
14 / 10Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Research Article


Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells

Ahmed Abdelfattah-Hassan, Doaa Ibrahim, Ayman A. Saleh, Asmaa T.Y. Kishawy, Reham H.A. Mohamed, Safaa I. Khater.


Cited By:1

Abstract
Background:
5-FU is an antimetabolic agent used for treating slowly growing solid tumors like breast and ovarian carcinoma. Thymoquinone (TQ) is a main biologically active constituent of Nigella sativa, it has been found to demonstrate anticancerous effects in several preclinical studies, and this is because TQ possesses multi-target nature. Stem cells-derived exosomes are in the spotlight of research and are promising tissue regenerative and anticancer cell-derived nanovesicles.
Aim:
Herein, we studied the antineoplastic effects of Exosomes derived from mammary stem cells (MaSCs-Exo) on breast cancer cells, alone or combined with TQ when compared to a breast cancer chemotherapeutic agent; 5-FU.
Methods:
Our approach included performing viability test and measuring the expression of pro-apoptotic gene (Bax), anti-apoptotic gene (BCL-2) and angiogenic gene (VEGF) on Human MCF-7 cells (breast adenocarcinoma cells), the MCF-7 cells were cultured and incubated with medium containing 5-FU (25 μg/mL), Thymoquinone (200 μg/mL), MaSCs-Exo (100 μg protein equivalent), a combination of TQ (200 μg/mL) and MaSCs-Exo (100 μg).
Results:
Our obtained results show that TQ and MaSCs-Exo each can effectively inhibit breast cancer cell line (MCF-7) proliferation and growth. Also, the results show that the combination of TQ and MaSCs-Exo had higher cytotoxic effects on MCF-7 breast cancer cells than TQ or 5-FU, alone.
Conclusion:
The present study shows promising anticancer potential of exosomes isolated from mammary stem cells; this effect was potentiated by adding thymoquinone with MaSCs-derived exosomes.

Key words: Mammary stem cells, 5-fluorouracil, Apoptosis, Breast cancer, Anti-tumor


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ahmed Abdelfattah-Hassan
Articles by Doaa Ibrahim
Articles by Ayman A. Saleh
Articles by Asmaa T.Y. Kishawy
Articles by Reham H.A. Mohamed
Articles by Safaa I. Khater
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Abdelfattah-Hassan A, Ibrahim D, Saleh AA, Kishawy AT, Mohamed RH, Khater SI. Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Vet J. 2024; 14((1) (Zagazig Veterinary Conference)): 525-533. doi:10.5455/OVJ.2024.v14.i1.47

Web Style

Abdelfattah-Hassan A, Ibrahim D, Saleh AA, Kishawy AT, Mohamed RH, Khater SI. Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. https://www.openveterinaryjournal.com/?mno=182418 [Access: November 08, 2024]. doi:10.5455/OVJ.2024.v14.i1.47

AMA (American Medical Association) Style

Abdelfattah-Hassan A, Ibrahim D, Saleh AA, Kishawy AT, Mohamed RH, Khater SI. Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Vet J. 2024; 14((1) (Zagazig Veterinary Conference)): 525-533. doi:10.5455/OVJ.2024.v14.i1.47


Vancouver/ICMJE Style

Abdelfattah-Hassan A, Ibrahim D, Saleh AA, Kishawy AT, Mohamed RH, Khater SI. Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Vet J. (2024), [cited November 08, 2024]; 14((1) (Zagazig Veterinary Conference)): 525-533. doi:10.5455/OVJ.2024.v14.i1.47


Harvard Style

Abdelfattah-Hassan, A., Ibrahim, . D., Saleh, . A. A., Kishawy, . A. T., Mohamed, . R. H. & Khater, . S. I. (2024) Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Vet J, 14 ((1) (Zagazig Veterinary Conference)), 525-533. doi:10.5455/OVJ.2024.v14.i1.47


Turabian Style

Abdelfattah-Hassan, Ahmed, Doaa Ibrahim, Ayman A. Saleh, Asmaa T.Y. Kishawy, Reham H.A. Mohamed, and Safaa I. Khater. 2024. Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Veterinary Journal, 14 ((1) (Zagazig Veterinary Conference)), 525-533. doi:10.5455/OVJ.2024.v14.i1.47


Chicago Style

Abdelfattah-Hassan, Ahmed, Doaa Ibrahim, Ayman A. Saleh, Asmaa T.Y. Kishawy, Reham H.A. Mohamed, and Safaa I. Khater. "Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells." Open Veterinary Journal 14 (2024), 525-533. doi:10.5455/OVJ.2024.v14.i1.47


MLA (The Modern Language Association) Style

Abdelfattah-Hassan, Ahmed, Doaa Ibrahim, Ayman A. Saleh, Asmaa T.Y. Kishawy, Reham H.A. Mohamed, and Safaa I. Khater. "Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells." Open Veterinary Journal 14.(1) (Zagazig Veterinary Conference) (2024), 525-533. Print. doi:10.5455/OVJ.2024.v14.i1.47


APA (American Psychological Association) Style

Abdelfattah-Hassan, A., Ibrahim, . D., Saleh, . A. A., Kishawy, . A. T., Mohamed, . R. H. & Khater, . S. I. (2024) Effects of 5-fluorouracil, thymoquinone and mammary stem cells’ exosomes on in vitro cultured breast cancer cells. Open Veterinary Journal, 14 ((1) (Zagazig Veterinary Conference)), 525-533. doi:10.5455/OVJ.2024.v14.i1.47


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly