E-ISSN 2218-6050 | ISSN 2226-4485
 

Research Article


Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae

Ariani Ariani, Husnul Khotimah, Arum Sulistyarini, Araisa Sabrina Daniaridevi.


Abstract
Background:
Oxygen deprivation (OD) is a critical condition that can lead to brain damage and even death. Current hypoxia management approaches are limited in effectiveness. Centella asiatica, known for its neuroprotective properties, offers a potential alternative for OD treatment.
Aim:
This study aims to investigate the neuroprotective effects of Centella asiatica (CA) on the expression of Brain-Derived Neurotrophic Factor (BDNF) and Vesicular Glutamate Transporter 1 (VGLUT1) in zebrafish larvae under oxygen-deficient conditions.
Methods:
Zebrafish embryos were subjected to low oxygen levels (1.5 mg/L) 0-2 hours post-fertilization (hpf) until three days post-fertilization (dpf), simulating the early stages of OD. Subsequent treatment involved varying concentrations of CA (1.25-5 µg/ml) up to nine days post-fertilization. The expression levels of BDNF and VGLUT1 were measured using PCR methods. Statistical analysis was conducted using Two-way ANOVA to evaluate the impact of CA on the expression of BDNF and VGLUT1 in zebrafish larvae aged 3 and 9 dpf in oxygen-deprived conditions.
Results:
CA significantly influenced the expression of BDNF and VGLUT1 under oxygen deprivation (p<0.001). An increase in BDNF expression (p<0.001) and a decrease in VGLUT1 (p<0.01) were observed in zebrafish larvae experiencing OD and treated with CA. There was no significant difference in BDNF and VGLUT1 expression across age variations in zebrafish larvae at 3 dpf and 9 dpf in the treatment groups (p>0.05). CA concentration of 2.5 µg/ml effectively enhanced BDNF and reduced VGLUT1 in 3-9 dpf zebrafish larvae.
Conclusion:
CA demonstrates potential as a neuroprotective agent, modulating increased BDNF expression and reduced VGLUT1 under OD conditions. These findings lay a foundation for further research in developing therapies for oxygen deficiency.

Key words: Centella asiatica, Neuroprotective, Neurotoxicity, Neuroplasticity


 
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How to Cite this Article
Pubmed Style

Ariani A, Khotimah H, Sulistyarini A, Daniaridevi AS. Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae. Open Vet J. 2024; 14(5): 1154-1160. doi:10.5455/OVJ.2024.v14.i5.9


Web Style

Ariani A, Khotimah H, Sulistyarini A, Daniaridevi AS. Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae. https://www.openveterinaryjournal.com/?mno=188192 [Access: June 03, 2024]. doi:10.5455/OVJ.2024.v14.i5.9


AMA (American Medical Association) Style

Ariani A, Khotimah H, Sulistyarini A, Daniaridevi AS. Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae. Open Vet J. 2024; 14(5): 1154-1160. doi:10.5455/OVJ.2024.v14.i5.9



Vancouver/ICMJE Style

Ariani A, Khotimah H, Sulistyarini A, Daniaridevi AS. Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae. Open Vet J. (2024), [cited June 03, 2024]; 14(5): 1154-1160. doi:10.5455/OVJ.2024.v14.i5.9



Harvard Style

Ariani, A., Khotimah, . H., Sulistyarini, . A. & Daniaridevi, . A. S. (2024) Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae. Open Vet J, 14 (5), 1154-1160. doi:10.5455/OVJ.2024.v14.i5.9



Turabian Style

Ariani, Ariani, Husnul Khotimah, Arum Sulistyarini, and Araisa Sabrina Daniaridevi. 2024. Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae. Open Veterinary Journal, 14 (5), 1154-1160. doi:10.5455/OVJ.2024.v14.i5.9



Chicago Style

Ariani, Ariani, Husnul Khotimah, Arum Sulistyarini, and Araisa Sabrina Daniaridevi. "Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae." Open Veterinary Journal 14 (2024), 1154-1160. doi:10.5455/OVJ.2024.v14.i5.9



MLA (The Modern Language Association) Style

Ariani, Ariani, Husnul Khotimah, Arum Sulistyarini, and Araisa Sabrina Daniaridevi. "Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae." Open Veterinary Journal 14.5 (2024), 1154-1160. Print. doi:10.5455/OVJ.2024.v14.i5.9



APA (American Psychological Association) Style

Ariani, A., Khotimah, . H., Sulistyarini, . A. & Daniaridevi, . A. S. (2024) Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae. Open Veterinary Journal, 14 (5), 1154-1160. doi:10.5455/OVJ.2024.v14.i5.9