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Research Article


Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep

Nanik Hidayatik, Sefi Lestyo Harini, Nafas Triwidiawati, Shalsa Izza Putri, Annise Proboningrat, Luviana Kristianingtyas, Aswin Rafif Khairullah, Lucia Tri Suwanti, Eka Pramyrtha Hestianah, Suryo Kuncorojakti, M. Gandul Atik Yuliani, Arindita Niatazya Novianti, Diky Ramdani, Ririn Siti Rahmatillah, Anuraga Jayanegara.


Abstract
Background:
The Trichuris eggs are collected from naturally infected sheep. Natural antihelmintics such as herbal medicines are needed as an alternative, such as natural compounds from endemic plants.
Aim:
This present study aims to evaluate the ovicidal activity and cytotoxicity effects of ethanolic extract of Curcuma longa (EECL) and Camelia sinensis (EECS) as a biological anthelmintic against the egg of Trichuris sp.
Methods:
The Trichuris eggs are collected from naturally infected sheep. CMC-Na solution 1% was used as control. The treatments were 0.12% EECL; 0.24% EECL; 0.15% EECS; 0.30% EECS; combination of 0.12% EECL and 0.30% EECS; combination 0.24% EECL; and 0.15% EECS. Ovicidal activity testing by microscopic examination of eggs treated using different concentrations of EECL extract, EECS and a combination of them. They were exposed for various times (7, 14, 21 and 28 days) and incubated at room temperature.
Results:
The study shows that combination of Curcuma longa extract and tea extract exhibits good ovicidal anthelmintic activity against the Trichuris sp. in sheep. Cytotoxicity examination using the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide (MTT) test. Based on MTT data processed using regression analysis, the obtained LC50 from administration of EECL, EECS and a combination of both in a ratio of 1:1, 2:2, 1:2 and 2:1. The combination of EECL extract and EECS with the highest concentration produced cell viability of 28.46%, 17.25%, 56.01%, and 46.47%, respectively.
Conclusion:
It can be concluded that the most cytotoxic ingredient is found in the combination of EECL and EECS (2:2) at 17.25% and the safest is in the ratio (1:2) at 56.01%.

Key words: Animal health, Camellia sinensis, Cytotoxicity, Curcuma longa, Ovicidal


 
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How to Cite this Article
Pubmed Style

Hidayatik N, Harini SL, Triwidiawati N, Putri SI, Proboningrat A, Kristianingtyas L, Khairullah AR, Suwanti LT, Hestianah EP, Kuncorojakti S, Yuliani MGA, Novianti AN, Ramdani D, RSR, Jayanegara A. Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep. Open Vet J. 2024; 14(6): 1467-1475. doi:10.5455/OVJ.2024.v14.i6.15

Web Style

Hidayatik N, Harini SL, Triwidiawati N, Putri SI, Proboningrat A, Kristianingtyas L, Khairullah AR, Suwanti LT, Hestianah EP, Kuncorojakti S, Yuliani MGA, Novianti AN, Ramdani D, RSR, Jayanegara A. Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep. https://www.openveterinaryjournal.com/?mno=197206 [Access: July 12, 2024]. doi:10.5455/OVJ.2024.v14.i6.15

AMA (American Medical Association) Style

Hidayatik N, Harini SL, Triwidiawati N, Putri SI, Proboningrat A, Kristianingtyas L, Khairullah AR, Suwanti LT, Hestianah EP, Kuncorojakti S, Yuliani MGA, Novianti AN, Ramdani D, RSR, Jayanegara A. Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep. Open Vet J. 2024; 14(6): 1467-1475. doi:10.5455/OVJ.2024.v14.i6.15


Vancouver/ICMJE Style

Hidayatik N, Harini SL, Triwidiawati N, Putri SI, Proboningrat A, Kristianingtyas L, Khairullah AR, Suwanti LT, Hestianah EP, Kuncorojakti S, Yuliani MGA, Novianti AN, Ramdani D, RSR, Jayanegara A. Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep. Open Vet J. (2024), [cited July 12, 2024]; 14(6): 1467-1475. doi:10.5455/OVJ.2024.v14.i6.15


Harvard Style

Hidayatik, N., Harini, . S. L., Triwidiawati, . N., Putri, . S. I., Proboningrat, . A., Kristianingtyas, . L., Khairullah, . A. R., Suwanti, . L. T., Hestianah, . E. P., Kuncorojakti, . S., Yuliani, . M. G. A., Novianti, . A. N., Ramdani, . D., , . R. S. R. & Jayanegara, . A. (2024) Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep. Open Vet J, 14 (6), 1467-1475. doi:10.5455/OVJ.2024.v14.i6.15


Turabian Style

Hidayatik, Nanik, Sefi Lestyo Harini, Nafas Triwidiawati, Shalsa Izza Putri, Annise Proboningrat, Luviana Kristianingtyas, Aswin Rafif Khairullah, Lucia Tri Suwanti, Eka Pramyrtha Hestianah, Suryo Kuncorojakti, M. Gandul Atik Yuliani, Arindita Niatazya Novianti, Diky Ramdani, Ririn Siti Rahmatillah, and Anuraga Jayanegara. 2024. Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep. Open Veterinary Journal, 14 (6), 1467-1475. doi:10.5455/OVJ.2024.v14.i6.15


Chicago Style

Hidayatik, Nanik, Sefi Lestyo Harini, Nafas Triwidiawati, Shalsa Izza Putri, Annise Proboningrat, Luviana Kristianingtyas, Aswin Rafif Khairullah, Lucia Tri Suwanti, Eka Pramyrtha Hestianah, Suryo Kuncorojakti, M. Gandul Atik Yuliani, Arindita Niatazya Novianti, Diky Ramdani, Ririn Siti Rahmatillah, and Anuraga Jayanegara. "Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep." Open Veterinary Journal 14 (2024), 1467-1475. doi:10.5455/OVJ.2024.v14.i6.15


MLA (The Modern Language Association) Style

Hidayatik, Nanik, Sefi Lestyo Harini, Nafas Triwidiawati, Shalsa Izza Putri, Annise Proboningrat, Luviana Kristianingtyas, Aswin Rafif Khairullah, Lucia Tri Suwanti, Eka Pramyrtha Hestianah, Suryo Kuncorojakti, M. Gandul Atik Yuliani, Arindita Niatazya Novianti, Diky Ramdani, Ririn Siti Rahmatillah, and Anuraga Jayanegara. "Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep." Open Veterinary Journal 14.6 (2024), 1467-1475. Print. doi:10.5455/OVJ.2024.v14.i6.15


APA (American Psychological Association) Style

Hidayatik, N., Harini, . S. L., Triwidiawati, . N., Putri, . S. I., Proboningrat, . A., Kristianingtyas, . L., Khairullah, . A. R., Suwanti, . L. T., Hestianah, . E. P., Kuncorojakti, . S., Yuliani, . M. G. A., Novianti, . A. N., Ramdani, . D., , . R. S. R. & Jayanegara, . A. (2024) Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep. Open Veterinary Journal, 14 (6), 1467-1475. doi:10.5455/OVJ.2024.v14.i6.15


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