Abstract
Background:
The increase in the intensity of agricultural production is associated with the action of various stress factors on the organism of birds, which can lead to negative consequences. Prevention of the development of stress conditions in farm birds, particularly broiler chickens, in industrial production is the most important task facing scientists and practitioners.
Aim:
The objective of this study was to investigate the effect of a combined probiotic preparation and zinc glycinate on the indicators of the immunity, biochemical parameters and antioxidant status.
Methods:
The study was conducted on broilers of Arbor Acres cross: the birds in the negative control group received a balanced feed mixture, a mineral and vitamin premix without zinc; the positive control group treated with an injection of cyclophosphamide and received the balanced feed with the mineral and vitamin premix without zinc; in experimental group I, broilers was treated with the injection of cyclophosphamide and received balanced feed, probiotic and zinc glycinate; in experimental group II, broilers received balanced feed, probiotic and zinc glycinate. At the end of the experiment, some blood from the wing vein was collected for evaluation of hematological and biochemical blood parameters using automatic morphological and biochemical analyzers, evaluation of immune status by enzyme immunoassay and antioxidant status by colorimetric method.
Results:
It was found that the developed strategy nutrition leveled the effects of immunosuppression - there was an increase in the level of lymphocytes, interleukin-4 and interleukin-10; level of cholesterol, triglycerides, glucose and total protein tended to the control values; there was a significant increase in serum interleukin-2 and interferon gamma; increase in total protein on the background of triglycerides decrease in broilers of experimental group II. An increase in the activity of superoxide dismutase and catalase against the background of a decrease in the level of malonic dialdehyde was revealed in the experimental groups.
Conclusion:
The developed strategy of broiler chicken nutrition can be used for successful protection of birds from immunodeficiency states, improvement of antioxidant status and maintenance of complete protein and lipid metabolism.

Key words: Poultry, Antioxidant status, Immune status, Immunodeficiency, Metabolism