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Research Article


Multi-drug resistance and phylogenetic analyses of Pseudomonas aeruginosa based on the 16S rRNA gene of isolates recovered from clinical samples and their susceptibility to silver-nanoparticle

Zeena Fouad Saleh, Shujon Hassan Jadaan, Hiba Shehab Ahmed, Saba Falah Klaif, Shahad Mazin Daham.


Abstract
Background:
Pseudomonas aeruginosa is a highly antimicrobial-resistant pathogen with a very narrow range of effective antibacterial agents. Therefore, finding alternative compounds is highly required, such as silver nanoparticles (AgNPs).
Aim:
The current study was conducted to identify the multi-drug resistance (MDR) profile and perform a phylogenetic analysis on Pseudomonas aeruginosa isolates recovered from clinical samples (human, cows, cats, and fish) and to study their susceptibility to AgNPs.
Methods:
40 samples were subjected to conventional cultivation and biochemical analyses to identify P. aeruginosa. Moreover, these isolates were tested for their antibiotic resistance profile and their response to AgNPs using disk diffusion methods. PCR and Sanger-based sequencing were performed using the 16S rRNA gene as a target.
Results:
The results showed that all isolates were resistant to cefixime and sensitive to meropenem. Conversely, the AgNPs were effective in producing larger zones of inhibition. The PCR revealed amplification of the target, and the sequencing and phylogenetic tree of four isolates revealed close similarity with global human sequences from different regions.
Conclusion:
The study reveals MDR characteristics of Pseudomonas aeruginosa. The isolates are highly susceptible to silver nanoparticles.

Key words: Alternative medicine, Antibiotic resistance, Multi-drug resistance, Nosocomial infection, Pseudomonas aeruginosa


 
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How to Cite this Article
Pubmed Style

Saleh ZF, Jadaan SH, Ahmed HS, Klaif SF, Daham SM. Multi-drug resistance and phylogenetic analyses of Pseudomonas aeruginosa based on the 16S rRNA gene of isolates recovered from clinical samples and their susceptibility to silver-nanoparticle. Open Vet J. 2024; 14(9): 2433-2440. doi:10.5455/OVJ.2024.v14.i9.32

Web Style

Saleh ZF, Jadaan SH, Ahmed HS, Klaif SF, Daham SM. Multi-drug resistance and phylogenetic analyses of Pseudomonas aeruginosa based on the 16S rRNA gene of isolates recovered from clinical samples and their susceptibility to silver-nanoparticle. https://www.openveterinaryjournal.com/?mno=205821 [Access: November 07, 2024]. doi:10.5455/OVJ.2024.v14.i9.32

AMA (American Medical Association) Style

Saleh ZF, Jadaan SH, Ahmed HS, Klaif SF, Daham SM. Multi-drug resistance and phylogenetic analyses of Pseudomonas aeruginosa based on the 16S rRNA gene of isolates recovered from clinical samples and their susceptibility to silver-nanoparticle. Open Vet J. 2024; 14(9): 2433-2440. doi:10.5455/OVJ.2024.v14.i9.32


Vancouver/ICMJE Style

Saleh ZF, Jadaan SH, Ahmed HS, Klaif SF, Daham SM. Multi-drug resistance and phylogenetic analyses of Pseudomonas aeruginosa based on the 16S rRNA gene of isolates recovered from clinical samples and their susceptibility to silver-nanoparticle. Open Vet J. (2024), [cited November 07, 2024]; 14(9): 2433-2440. doi:10.5455/OVJ.2024.v14.i9.32


Harvard Style

Saleh, Z. F., Jadaan, . S. H., Ahmed, . H. S., Klaif, . S. F. & Daham, . S. M. (2024) Multi-drug resistance and phylogenetic analyses of Pseudomonas aeruginosa based on the 16S rRNA gene of isolates recovered from clinical samples and their susceptibility to silver-nanoparticle. Open Vet J, 14 (9), 2433-2440. doi:10.5455/OVJ.2024.v14.i9.32


Turabian Style

Saleh, Zeena Fouad, Shujon Hassan Jadaan, Hiba Shehab Ahmed, Saba Falah Klaif, and Shahad Mazin Daham. 2024. Multi-drug resistance and phylogenetic analyses of Pseudomonas aeruginosa based on the 16S rRNA gene of isolates recovered from clinical samples and their susceptibility to silver-nanoparticle. Open Veterinary Journal, 14 (9), 2433-2440. doi:10.5455/OVJ.2024.v14.i9.32


Chicago Style

Saleh, Zeena Fouad, Shujon Hassan Jadaan, Hiba Shehab Ahmed, Saba Falah Klaif, and Shahad Mazin Daham. "Multi-drug resistance and phylogenetic analyses of Pseudomonas aeruginosa based on the 16S rRNA gene of isolates recovered from clinical samples and their susceptibility to silver-nanoparticle." Open Veterinary Journal 14 (2024), 2433-2440. doi:10.5455/OVJ.2024.v14.i9.32


MLA (The Modern Language Association) Style

Saleh, Zeena Fouad, Shujon Hassan Jadaan, Hiba Shehab Ahmed, Saba Falah Klaif, and Shahad Mazin Daham. "Multi-drug resistance and phylogenetic analyses of Pseudomonas aeruginosa based on the 16S rRNA gene of isolates recovered from clinical samples and their susceptibility to silver-nanoparticle." Open Veterinary Journal 14.9 (2024), 2433-2440. Print. doi:10.5455/OVJ.2024.v14.i9.32


APA (American Psychological Association) Style

Saleh, Z. F., Jadaan, . S. H., Ahmed, . H. S., Klaif, . S. F. & Daham, . S. M. (2024) Multi-drug resistance and phylogenetic analyses of Pseudomonas aeruginosa based on the 16S rRNA gene of isolates recovered from clinical samples and their susceptibility to silver-nanoparticle. Open Veterinary Journal, 14 (9), 2433-2440. doi:10.5455/OVJ.2024.v14.i9.32


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