E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article

Online Publishing Date: 30 / 06 / 2016



The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures

M. Garvey, G. Coughlan, N. Murphy, N. Rowan.


Cited By:5

Abstract
The presence of pathogenic organisms namely parasite species and bacteria in biofilms in veterinary settings, is a public health concern in relation to human and animal exposure. Veterinary clinics represent a significant risk factor for the transfer of pathogens from housed animals to humans, especially in cases of wound infection and the shedding of faecal matter. This study aims to provide a means of detecting veterinary relevant parasite species in bacterial biofilms, and to provide a means of disinfecting these biofilms. A real time PCR assay was utilized to detect parasite DNA in Bacillus cereus biofilms on stainless steel and PVC surfaces. Results show that both Cryptosporidium and Giardia attach to biofilms in large numbers (100-1000 oo/cysts) in as little as 72 hours. Pulsed light successfully inactivated all test species (Listeria, Salmonella, Bacillus, Escherichia) in planktonic and biofilm form with an increase in inactivation for every increase in UV dose.

Key words: Biofilms, Cryptosporidium, Giardia, PCR, Veterinary


 
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How to Cite this Article
Pubmed Style

M. Garvey, G. Coughlan, N. Murphy, N. Rowan. The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures. Open Vet J. 2016; 6(1): 15-22. doi:10.4314/ovj.v6i1.3


Web Style

M. Garvey, G. Coughlan, N. Murphy, N. Rowan. The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures. https://www.openveterinaryjournal.com/?mno=234356 [Access: February 13, 2024]. doi:10.4314/ovj.v6i1.3


AMA (American Medical Association) Style

M. Garvey, G. Coughlan, N. Murphy, N. Rowan. The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures. Open Vet J. 2016; 6(1): 15-22. doi:10.4314/ovj.v6i1.3



Vancouver/ICMJE Style

M. Garvey, G. Coughlan, N. Murphy, N. Rowan. The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures. Open Vet J. (2016), [cited February 13, 2024]; 6(1): 15-22. doi:10.4314/ovj.v6i1.3



Harvard Style

M. Garvey, G. Coughlan, N. Murphy, N. Rowan (2016) The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures. Open Vet J, 6 (1), 15-22. doi:10.4314/ovj.v6i1.3



Turabian Style

M. Garvey, G. Coughlan, N. Murphy, N. Rowan. 2016. The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures. Open Veterinary Journal, 6 (1), 15-22. doi:10.4314/ovj.v6i1.3



Chicago Style

M. Garvey, G. Coughlan, N. Murphy, N. Rowan. "The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures." Open Veterinary Journal 6 (2016), 15-22. doi:10.4314/ovj.v6i1.3



MLA (The Modern Language Association) Style

M. Garvey, G. Coughlan, N. Murphy, N. Rowan. "The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures." Open Veterinary Journal 6.1 (2016), 15-22. Print. doi:10.4314/ovj.v6i1.3



APA (American Psychological Association) Style

M. Garvey, G. Coughlan, N. Murphy, N. Rowan (2016) The pulsed light inactivation of veterinary relevant microbial biofilms and the use of a RTPCR assay to detect parasite species within biofilm structures. Open Veterinary Journal, 6 (1), 15-22. doi:10.4314/ovj.v6i1.3