E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 7
15 / 7Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form

Research Article


Molecular characterization and phylogenetic analysis of the lumpy skin disease virus isolated in Al-Zawiya, Western Libya

Ahmad A. A. Saad, Mohammed A. Murshid, Marium M. Hussein, Tarek A. Zahmol, Najwa A. I. Abdulsalam, Esmaiel I. F. Saad, Osama N. Elwaer, Esmahan M. Ismaeil, Abderrahman. J. Jbeel.


Abstract
Background:
Cattle are naturally susceptible to the viral disease lumpy skin disease (LSD), which has become a significant concern for the livestock industry in Libya since its first emergence in 2023. Originating from Africa, it rapidly spread across the country, facilitated by the movement of animals both within Libya and from neighboring regions.

Aim:
This investigation determined the molecular features and the phylogenetic analysis of LSD virus (LSDV) isolated from cattle from Al-Zawiya, Western Libya.

Methods:
Polymerase chain reaction (PCR) was performed on 14 collected samples. Real-time PCR was employed to detect and amplify specific viral DNA sequences, confirming the presence and identifying the genetic profile of LSDV.

Results:
Results confirmed the presence of LSDV in 13 of 14 samples, with subsequent sequencing revealing distinct genetic markers characteristic of the virus strains in Libya. The findings also revealed that the LSDV responsible for these outbreaks is closely related to LSDV Sudan/06-Obied_GU119938, LSDV Egypt/89-Ismalia_GU119947, and LSDV Serbia/Buj/2016_KY702007. Additionally, genetic analysis revealed that the Libyan LSDV strain is unique among all LSDV isolates due to an additional single -nucleotide polymorphism at position 111 (C→A), which may affect the efficacy of immunization efforts to control the disease.

Conclusion:
This study serves as a valuable resource for understanding the genetic characteristics of LSDV in Libya and may assist in selecting the most appropriate vaccine strain for disease control.

Key words: Lumpy skin disease, PCR, Sequence, Outbreak, Libya


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ahmad A. A. Saad
Articles by Mohammed A. Murshid
Articles by Marium M. Hussein
Articles by Tarek A. Zahmol
Articles by Najwa A. I. Abdulsalam
Articles by Esmaiel I. F. Saad
Articles by Osama N. Elwaer
Articles by Esmahan M. Ismaeil
Articles by Abderrahman. J. Jbeel
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Saad AAA, Murshid MA, Hussein MM, Zahmol TA, Abdulsalam NAI, Saad EIF, Elwaer ON, Ismaeil EM, Jbeel AJ. Molecular characterization and phylogenetic analysis of the lumpy skin disease virus isolated in Al-Zawiya, Western Libya. Open Vet. J.. 2025; 15(7): 3044-3053. doi:10.5455/OVJ.2025.v15.i7.15

Web Style

Saad AAA, Murshid MA, Hussein MM, Zahmol TA, Abdulsalam NAI, Saad EIF, Elwaer ON, Ismaeil EM, Jbeel AJ. Molecular characterization and phylogenetic analysis of the lumpy skin disease virus isolated in Al-Zawiya, Western Libya. https://www.openveterinaryjournal.com/?mno=235282 [Access: August 29, 2025]. doi:10.5455/OVJ.2025.v15.i7.15

AMA (American Medical Association) Style

Saad AAA, Murshid MA, Hussein MM, Zahmol TA, Abdulsalam NAI, Saad EIF, Elwaer ON, Ismaeil EM, Jbeel AJ. Molecular characterization and phylogenetic analysis of the lumpy skin disease virus isolated in Al-Zawiya, Western Libya. Open Vet. J.. 2025; 15(7): 3044-3053. doi:10.5455/OVJ.2025.v15.i7.15


Vancouver/ICMJE Style

Saad AAA, Murshid MA, Hussein MM, Zahmol TA, Abdulsalam NAI, Saad EIF, Elwaer ON, Ismaeil EM, Jbeel AJ. Molecular characterization and phylogenetic analysis of the lumpy skin disease virus isolated in Al-Zawiya, Western Libya. Open Vet. J.. (2025), [cited August 29, 2025]; 15(7): 3044-3053. doi:10.5455/OVJ.2025.v15.i7.15


Harvard Style

Saad, A. A. A., Murshid, . M. A., Hussein, . M. M., Zahmol, . T. A., Abdulsalam, . N. A. I., Saad, . E. I. F., Elwaer, . O. N., Ismaeil, . E. M. & Jbeel, . A. J. (2025) Molecular characterization and phylogenetic analysis of the lumpy skin disease virus isolated in Al-Zawiya, Western Libya. Open Vet. J., 15 (7), 3044-3053. doi:10.5455/OVJ.2025.v15.i7.15


Turabian Style

Saad, Ahmad A. A., Mohammed A. Murshid, Marium M. Hussein, Tarek A. Zahmol, Najwa A. I. Abdulsalam, Esmaiel I. F. Saad, Osama N. Elwaer, Esmahan M. Ismaeil, and Abderrahman. J. Jbeel. 2025. Molecular characterization and phylogenetic analysis of the lumpy skin disease virus isolated in Al-Zawiya, Western Libya. Open Veterinary Journal, 15 (7), 3044-3053. doi:10.5455/OVJ.2025.v15.i7.15


Chicago Style

Saad, Ahmad A. A., Mohammed A. Murshid, Marium M. Hussein, Tarek A. Zahmol, Najwa A. I. Abdulsalam, Esmaiel I. F. Saad, Osama N. Elwaer, Esmahan M. Ismaeil, and Abderrahman. J. Jbeel. "Molecular characterization and phylogenetic analysis of the lumpy skin disease virus isolated in Al-Zawiya, Western Libya." Open Veterinary Journal 15 (2025), 3044-3053. doi:10.5455/OVJ.2025.v15.i7.15


MLA (The Modern Language Association) Style

Saad, Ahmad A. A., Mohammed A. Murshid, Marium M. Hussein, Tarek A. Zahmol, Najwa A. I. Abdulsalam, Esmaiel I. F. Saad, Osama N. Elwaer, Esmahan M. Ismaeil, and Abderrahman. J. Jbeel. "Molecular characterization and phylogenetic analysis of the lumpy skin disease virus isolated in Al-Zawiya, Western Libya." Open Veterinary Journal 15.7 (2025), 3044-3053. Print. doi:10.5455/OVJ.2025.v15.i7.15


APA (American Psychological Association) Style

Saad, A. A. A., Murshid, . M. A., Hussein, . M. M., Zahmol, . T. A., Abdulsalam, . N. A. I., Saad, . E. I. F., Elwaer, . O. N., Ismaeil, . E. M. & Jbeel, . A. J. (2025) Molecular characterization and phylogenetic analysis of the lumpy skin disease virus isolated in Al-Zawiya, Western Libya. Open Veterinary Journal, 15 (7), 3044-3053. doi:10.5455/OVJ.2025.v15.i7.15


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldaghayes.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly