Abstract
Background:
Zinc oxide nanoparticles (ZnO NPs) have low toxicity and utilized extensively in medicine. The properties of zinc oxide nanoparticles include the promotion of growth, the modulation of the immune system, and the regulation of animal reproduction.

Aim:
An evaluation of the renal and hepatic function of lambs subjected to zinc oxide nanoparticle treatment

Methods:
Fifteen apparently healthy local breed lambs, aged between seven and nine months, were utilized in the present study. The lambs were divided randomly into three equal groups. The control group (Group I) received water and glycerol only, the treatment group (Group II) received 3.5 mg/kg of ZnO NPs orally, and the third group (Group III) received 7 mg/kg of ZnO NPs orally once daily for a period of 90 days. A comprehensive biochemical analysis encompasses a range of essential parameters, including AST, ALP, ALP, BU and TSB.

Results:
A significant increase in T.S.B. was observed among the control group (I) in comparison with the initial baseline. Furthermore, a significant increase in T.S.B. was noted in group I when it was incorporated with groups II and III at the end of the study. A significant increase in serum AST and ALK was observed among groups I, II and III at the study's conclusion in comparison with the initial baseline. However, no significant changes were detected between these groups. Conversely, serum ALP values exhibited a significant decrease at the study's conclusion in comparison with the baseline. There was a substantial increase in serum urea and creatinine levels at the end of the study in all groups when compared with the baseline.

Conclusion:
Due to the different results of toxicological studies on experimental animals. Current recommends carrying out more studies to clarify the toxic effects, in order to install the appropriate doses for animals to determine the desired benefit of Nano-Zinc to reach logical conclusions.

Key words: Kidney, Liver, Function, Lambs, Zinc oxide nanoparticles