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Research Article


Molecular detection of blaSHV gene in multidrug resistance of Klebsiella pneumoniae isolated from chicken egg shell swab from a traditional market in Surabaya

Azhari Athaillah Sulaiman, Mustofa Helmi Effendi, Leonardo Reza Srimaryanto, Aswin Rafif Khairullah, Pitaloka Kurniasari, Wiwiek Tyasningsih, Ikechukwu Benjamin Moses, Riza Zainuddin Ahmad, Saifur Rehman, Budiastuti Budiastuti, Daniah Ashri Afnani, Sheila Marty Yanestria, Katty Hendriana Priscilia Riwu, Agus Widodo.


Abstract
Background:
Contamination with Klebsiella pneumoniae in food ingredients, including eggs, causes various dangers because it threatens public health, because it acts as a multidrug resistance (MDR) bacteria, especially the extendedspectrum beta-lactamase (ESBL) strain. The ESBL blaSHV gene is part of a broad-spectrum ESBL that is often found in Gram-negative bacteria.

Aim:
This study aimed to identify the ESBL blaSHV gene in K. pneumoniae MDR from chicken eggshells.

Methods:
This study used 160 samples of chicken eggshell swabs isolated on 1% BPW media from 10 traditional Surabaya markets. Samples were isolated using MCA media and were identified using Gram staining and biochemical tests. Detection of MDR using Muller-Hinton Agar.

Results:
Confirmation of ESBL in multidrug-resistant (MDR) isolates was performed using polymerase chain reaction to detect ESBL genes. The results showed that the isolation and identification of K. pneumoniae bacteria were 25.62% (41/160). Amoxicillin antibiotics showed the highest level of resistance at a percentage of 100% (41/41), followed by antibiotic resistance to erythromycin (90.24% (37/41), Streptomycin antibiotics were 26.82% (11/41), ciprofloxacin (14.63% (6/41), and Tetracycline antibiotic resistance was 7.31% (3/41). The results of MDR from K. pneumoniae showed 34.14% (14/41) of the isolates were then tested by PCR, which showed positive results for the blaSHV gene of 71.42% (10/14).

Conclusion:
The data from this study confirm the existence of K. pneumoniae bacteria isolated from egg shell swabs carrying the blaSHV gene from MDR isolates.

Key words: K. pneumoniae, MDR, ESBL, Public health, Egg shell


 
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How to Cite this Article
Pubmed Style

Sulaiman AA, Effendi MH, Srimaryanto LR, Khairullah AR, Kurniasari P, Tyasningsih W, Moses IB, Ahmad RZ, Rehman S, Budiastuti B, Afnani DA, Yanestria SM, Riwu KHP, Widodo A. Molecular detection of blaSHV gene in multidrug resistance of Klebsiella pneumoniae isolated from chicken egg shell swab from a traditional market in Surabaya. Open Vet J. 2025; 15(5): 2193-2205. doi:10.5455/OVJ.2025.v15.i5.37

Web Style

Sulaiman AA, Effendi MH, Srimaryanto LR, Khairullah AR, Kurniasari P, Tyasningsih W, Moses IB, Ahmad RZ, Rehman S, Budiastuti B, Afnani DA, Yanestria SM, Riwu KHP, Widodo A. Molecular detection of blaSHV gene in multidrug resistance of Klebsiella pneumoniae isolated from chicken egg shell swab from a traditional market in Surabaya. https://www.openveterinaryjournal.com/?mno=244245 [Access: June 06, 2025]. doi:10.5455/OVJ.2025.v15.i5.37

AMA (American Medical Association) Style

Sulaiman AA, Effendi MH, Srimaryanto LR, Khairullah AR, Kurniasari P, Tyasningsih W, Moses IB, Ahmad RZ, Rehman S, Budiastuti B, Afnani DA, Yanestria SM, Riwu KHP, Widodo A. Molecular detection of blaSHV gene in multidrug resistance of Klebsiella pneumoniae isolated from chicken egg shell swab from a traditional market in Surabaya. Open Vet J. 2025; 15(5): 2193-2205. doi:10.5455/OVJ.2025.v15.i5.37


Vancouver/ICMJE Style

Sulaiman AA, Effendi MH, Srimaryanto LR, Khairullah AR, Kurniasari P, Tyasningsih W, Moses IB, Ahmad RZ, Rehman S, Budiastuti B, Afnani DA, Yanestria SM, Riwu KHP, Widodo A. Molecular detection of blaSHV gene in multidrug resistance of Klebsiella pneumoniae isolated from chicken egg shell swab from a traditional market in Surabaya. Open Vet J. (2025), [cited June 06, 2025]; 15(5): 2193-2205. doi:10.5455/OVJ.2025.v15.i5.37


Harvard Style

Sulaiman, A. A., Effendi, . M. H., Srimaryanto, . L. R., Khairullah, . A. R., Kurniasari, . P., Tyasningsih, . W., Moses, . I. B., Ahmad, . R. Z., Rehman, . S., Budiastuti, . B., Afnani, . D. A., Yanestria, . S. M., Riwu, . K. H. P. & Widodo, . A. (2025) Molecular detection of blaSHV gene in multidrug resistance of Klebsiella pneumoniae isolated from chicken egg shell swab from a traditional market in Surabaya. Open Vet J, 15 (5), 2193-2205. doi:10.5455/OVJ.2025.v15.i5.37


Turabian Style

Sulaiman, Azhari Athaillah, Mustofa Helmi Effendi, Leonardo Reza Srimaryanto, Aswin Rafif Khairullah, Pitaloka Kurniasari, Wiwiek Tyasningsih, Ikechukwu Benjamin Moses, Riza Zainuddin Ahmad, Saifur Rehman, Budiastuti Budiastuti, Daniah Ashri Afnani, Sheila Marty Yanestria, Katty Hendriana Priscilia Riwu, and Agus Widodo. 2025. Molecular detection of blaSHV gene in multidrug resistance of Klebsiella pneumoniae isolated from chicken egg shell swab from a traditional market in Surabaya. Open Veterinary Journal, 15 (5), 2193-2205. doi:10.5455/OVJ.2025.v15.i5.37


Chicago Style

Sulaiman, Azhari Athaillah, Mustofa Helmi Effendi, Leonardo Reza Srimaryanto, Aswin Rafif Khairullah, Pitaloka Kurniasari, Wiwiek Tyasningsih, Ikechukwu Benjamin Moses, Riza Zainuddin Ahmad, Saifur Rehman, Budiastuti Budiastuti, Daniah Ashri Afnani, Sheila Marty Yanestria, Katty Hendriana Priscilia Riwu, and Agus Widodo. "Molecular detection of blaSHV gene in multidrug resistance of Klebsiella pneumoniae isolated from chicken egg shell swab from a traditional market in Surabaya." Open Veterinary Journal 15 (2025), 2193-2205. doi:10.5455/OVJ.2025.v15.i5.37


MLA (The Modern Language Association) Style

Sulaiman, Azhari Athaillah, Mustofa Helmi Effendi, Leonardo Reza Srimaryanto, Aswin Rafif Khairullah, Pitaloka Kurniasari, Wiwiek Tyasningsih, Ikechukwu Benjamin Moses, Riza Zainuddin Ahmad, Saifur Rehman, Budiastuti Budiastuti, Daniah Ashri Afnani, Sheila Marty Yanestria, Katty Hendriana Priscilia Riwu, and Agus Widodo. "Molecular detection of blaSHV gene in multidrug resistance of Klebsiella pneumoniae isolated from chicken egg shell swab from a traditional market in Surabaya." Open Veterinary Journal 15.5 (2025), 2193-2205. Print. doi:10.5455/OVJ.2025.v15.i5.37


APA (American Psychological Association) Style

Sulaiman, A. A., Effendi, . M. H., Srimaryanto, . L. R., Khairullah, . A. R., Kurniasari, . P., Tyasningsih, . W., Moses, . I. B., Ahmad, . R. Z., Rehman, . S., Budiastuti, . B., Afnani, . D. A., Yanestria, . S. M., Riwu, . K. H. P. & Widodo, . A. (2025) Molecular detection of blaSHV gene in multidrug resistance of Klebsiella pneumoniae isolated from chicken egg shell swab from a traditional market in Surabaya. Open Veterinary Journal, 15 (5), 2193-2205. doi:10.5455/OVJ.2025.v15.i5.37


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