E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 8
15 / 8Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form

Research Article


Comparative study of mortalities, clinical manifestations, antioxidant-associated genes, and histopathological degeneration upon experiment infection by Aeromonas hydrophila in Cyprinus carpio and Oreochromis niloticus

Nadia A. H. Al- Shammari, Khalidah S. Al-niaeem, Adnan B. Al-hawash, Mosleh Mohammad Abomughaid, Aaser M. Abdelazim, Preetham Elumalai, El-sayed Hemdan Eissa, Heba H. Mahboub, Heba Allah M. Elbaghdady.


Abstract
Background:
Aeromonas hydrophila is a pathogenic bacterial infection threatening the aquaculture industry.

Aim:
The current work was performed to scrutinize the impacts of A. hydrophila infection on the occurrence of mortalities, altering the clinical picture of fish, antioxidants-associated gene expression, and histopathological architecture of the Cyprinus carpio and Oreochromis niloticus.

Methods:
Juvenile fishes including C. carpio, n = 60, and O. niloticus, n = 60 were haphazardly alienated into the control group (uninfected) and infected group with 100 μl of A. hydrophila. Mortalities and clinical signs were recorded during the experiment. Samples of liver, kidney, and spleen were collected post-infection for 7 days to monitor the expression of superoxide dismutase, glutathione peroxidase, and catalase genes, plus the assessment of histopathology.

Results:
The rate of mortalities was higher in C. carpio compared to O. niloticus. Additionally, infected O. niloticus revealed red eyes and erythema, while C. carpio showed exophthalmia and severe skin ulceration with exposure to viscera. The gene expression indicators showed that A. hydrophila significantly declined on the 1st day and the 7th day after infection, but significantly increased (p < 0.05) on the 3rd day compared to their respective control groups for C. carpio. Meanwhile, O. niloticus significantly regulated gene expression (p < 0.05) in the control groups. The histological picture indicated that the liver is the most affected organ. Moreover, C. carpio exhibited more disruption in histological architecture related to O. niloticus.

Conclusion:
Overall, A. hydrophila is extremely virulent and results in higher mortalities, profound clinical manifestations, down-regulation in the gene expression, and histopathological alterations in the hepato-renal and splenic tissues of C. carpio and O. niloticus. Cyprinus carpio was more adversely affected by the infection compared to O. niloticus. However, O. niloticus revealed higher gene expression, particularly in the spleen, and the genes were more expressed in comparison to C. carpio.

Key words: Gene expression, Histopathology, Aeromonas hydrophila, Cyprinus carpio, Oreochromis niloticus


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Nadia A. H. Al- Shammari
Articles by Khalidah S. AL-Niaeem
Articles by Adnan B. Al-Hawash
Articles by Mosleh Mohammad Abomughaid
Articles by Aaser M. Abdelazim
Articles by Preetham Elumalai
Articles by El-Sayed Hemdan Eissa
Articles by Heba H. Mahboub
Articles by Heba Allah M. Elbaghdady
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Shammari NAHA, Al-niaeem KS, Al-hawash AB, Abomughaid MM, Abdelazim AM, Elumalai P, Eissa EH, Mahboub HH, Elbaghdady HAM. Comparative study of mortalities, clinical manifestations, antioxidant-associated genes, and histopathological degeneration upon experiment infection by Aeromonas hydrophila in Cyprinus carpio and Oreochromis niloticus. Open Vet. J.. 2025; 15(7): 3012-3023. doi:10.5455/OVJ.2025.v15.i7.12

Web Style

Shammari NAHA, Al-niaeem KS, Al-hawash AB, Abomughaid MM, Abdelazim AM, Elumalai P, Eissa EH, Mahboub HH, Elbaghdady HAM. Comparative study of mortalities, clinical manifestations, antioxidant-associated genes, and histopathological degeneration upon experiment infection by Aeromonas hydrophila in Cyprinus carpio and Oreochromis niloticus. https://www.openveterinaryjournal.com/?mno=255558 [Access: August 31, 2025]. doi:10.5455/OVJ.2025.v15.i7.12

AMA (American Medical Association) Style

Shammari NAHA, Al-niaeem KS, Al-hawash AB, Abomughaid MM, Abdelazim AM, Elumalai P, Eissa EH, Mahboub HH, Elbaghdady HAM. Comparative study of mortalities, clinical manifestations, antioxidant-associated genes, and histopathological degeneration upon experiment infection by Aeromonas hydrophila in Cyprinus carpio and Oreochromis niloticus. Open Vet. J.. 2025; 15(7): 3012-3023. doi:10.5455/OVJ.2025.v15.i7.12


Vancouver/ICMJE Style

Shammari NAHA, Al-niaeem KS, Al-hawash AB, Abomughaid MM, Abdelazim AM, Elumalai P, Eissa EH, Mahboub HH, Elbaghdady HAM. Comparative study of mortalities, clinical manifestations, antioxidant-associated genes, and histopathological degeneration upon experiment infection by Aeromonas hydrophila in Cyprinus carpio and Oreochromis niloticus. Open Vet. J.. (2025), [cited August 31, 2025]; 15(7): 3012-3023. doi:10.5455/OVJ.2025.v15.i7.12


Harvard Style

Shammari, N. A. H. A., Al-niaeem, . K. S., Al-hawash, . A. B., Abomughaid, . M. M., Abdelazim, . A. M., Elumalai, . P., Eissa, . E. H., Mahboub, . H. H. & Elbaghdady, . H. A. M. (2025) Comparative study of mortalities, clinical manifestations, antioxidant-associated genes, and histopathological degeneration upon experiment infection by Aeromonas hydrophila in Cyprinus carpio and Oreochromis niloticus. Open Vet. J., 15 (7), 3012-3023. doi:10.5455/OVJ.2025.v15.i7.12


Turabian Style

Shammari, Nadia A. H. Al-, Khalidah S. Al-niaeem, Adnan B. Al-hawash, Mosleh Mohammad Abomughaid, Aaser M. Abdelazim, Preetham Elumalai, El-sayed Hemdan Eissa, Heba H. Mahboub, and Heba Allah M. Elbaghdady. 2025. Comparative study of mortalities, clinical manifestations, antioxidant-associated genes, and histopathological degeneration upon experiment infection by Aeromonas hydrophila in Cyprinus carpio and Oreochromis niloticus. Open Veterinary Journal, 15 (7), 3012-3023. doi:10.5455/OVJ.2025.v15.i7.12


Chicago Style

Shammari, Nadia A. H. Al-, Khalidah S. Al-niaeem, Adnan B. Al-hawash, Mosleh Mohammad Abomughaid, Aaser M. Abdelazim, Preetham Elumalai, El-sayed Hemdan Eissa, Heba H. Mahboub, and Heba Allah M. Elbaghdady. "Comparative study of mortalities, clinical manifestations, antioxidant-associated genes, and histopathological degeneration upon experiment infection by Aeromonas hydrophila in Cyprinus carpio and Oreochromis niloticus." Open Veterinary Journal 15 (2025), 3012-3023. doi:10.5455/OVJ.2025.v15.i7.12


MLA (The Modern Language Association) Style

Shammari, Nadia A. H. Al-, Khalidah S. Al-niaeem, Adnan B. Al-hawash, Mosleh Mohammad Abomughaid, Aaser M. Abdelazim, Preetham Elumalai, El-sayed Hemdan Eissa, Heba H. Mahboub, and Heba Allah M. Elbaghdady. "Comparative study of mortalities, clinical manifestations, antioxidant-associated genes, and histopathological degeneration upon experiment infection by Aeromonas hydrophila in Cyprinus carpio and Oreochromis niloticus." Open Veterinary Journal 15.7 (2025), 3012-3023. Print. doi:10.5455/OVJ.2025.v15.i7.12


APA (American Psychological Association) Style

Shammari, N. A. H. A., Al-niaeem, . K. S., Al-hawash, . A. B., Abomughaid, . M. M., Abdelazim, . A. M., Elumalai, . P., Eissa, . E. H., Mahboub, . H. H. & Elbaghdady, . H. A. M. (2025) Comparative study of mortalities, clinical manifestations, antioxidant-associated genes, and histopathological degeneration upon experiment infection by Aeromonas hydrophila in Cyprinus carpio and Oreochromis niloticus. Open Veterinary Journal, 15 (7), 3012-3023. doi:10.5455/OVJ.2025.v15.i7.12


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldaghayes.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly