E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Effects of storage temperature on the Quantity and Integrity of Genomic DNA Extracted from Mice Tissues: A Comparison of Recovery Methods

Huda Hassan AL-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aish M Zaidi, Refaat M Tabagh, Mohamed A AL-Griw.


Cited By:20

Abstract
Background & Study Aim: Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Methods: Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20C, 4C, 25C and 40C. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin Blood & Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Results: Both methods, Chelex-100 and NucleoSpin Blood & Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex100 method yielding a greater quantity (P < 0.045) than the kit. At -20C, 4C, and 25C temperatures, the concentration of DNA yield was numerically lower than at 40C. The NucleoSpin Blood & Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. Conclusion: the Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.

Key words: Forensic science, DNA profiling, challenging samples, DNA degradation, DNA extraction, Purity, Temperature.


 
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How to Cite this Article
Pubmed Style

Huda Hassan AL-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aish M Zaidi, Refaat M Tabagh, Mohamed A AL-Griw. Effects of storage temperature on the Quantity and Integrity of Genomic DNA Extracted from Mice Tissues: A Comparison of Recovery Methods. Open Vet J. 2017; 7(3): 239-243. doi:10.4314/ovj.v7i3.7


Web Style

Huda Hassan AL-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aish M Zaidi, Refaat M Tabagh, Mohamed A AL-Griw. Effects of storage temperature on the Quantity and Integrity of Genomic DNA Extracted from Mice Tissues: A Comparison of Recovery Methods. https://www.openveterinaryjournal.com/?mno=266244 [Access: February 13, 2024]. doi:10.4314/ovj.v7i3.7


AMA (American Medical Association) Style

Huda Hassan AL-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aish M Zaidi, Refaat M Tabagh, Mohamed A AL-Griw. Effects of storage temperature on the Quantity and Integrity of Genomic DNA Extracted from Mice Tissues: A Comparison of Recovery Methods. Open Vet J. 2017; 7(3): 239-243. doi:10.4314/ovj.v7i3.7



Vancouver/ICMJE Style

Huda Hassan AL-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aish M Zaidi, Refaat M Tabagh, Mohamed A AL-Griw. Effects of storage temperature on the Quantity and Integrity of Genomic DNA Extracted from Mice Tissues: A Comparison of Recovery Methods. Open Vet J. (2017), [cited February 13, 2024]; 7(3): 239-243. doi:10.4314/ovj.v7i3.7



Harvard Style

Huda Hassan AL-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aish M Zaidi, Refaat M Tabagh, Mohamed A AL-Griw (2017) Effects of storage temperature on the Quantity and Integrity of Genomic DNA Extracted from Mice Tissues: A Comparison of Recovery Methods. Open Vet J, 7 (3), 239-243. doi:10.4314/ovj.v7i3.7



Turabian Style

Huda Hassan AL-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aish M Zaidi, Refaat M Tabagh, Mohamed A AL-Griw. 2017. Effects of storage temperature on the Quantity and Integrity of Genomic DNA Extracted from Mice Tissues: A Comparison of Recovery Methods. Open Veterinary Journal, 7 (3), 239-243. doi:10.4314/ovj.v7i3.7



Chicago Style

Huda Hassan AL-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aish M Zaidi, Refaat M Tabagh, Mohamed A AL-Griw. "Effects of storage temperature on the Quantity and Integrity of Genomic DNA Extracted from Mice Tissues: A Comparison of Recovery Methods." Open Veterinary Journal 7 (2017), 239-243. doi:10.4314/ovj.v7i3.7



MLA (The Modern Language Association) Style

Huda Hassan AL-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aish M Zaidi, Refaat M Tabagh, Mohamed A AL-Griw. "Effects of storage temperature on the Quantity and Integrity of Genomic DNA Extracted from Mice Tissues: A Comparison of Recovery Methods." Open Veterinary Journal 7.3 (2017), 239-243. Print. doi:10.4314/ovj.v7i3.7



APA (American Psychological Association) Style

Huda Hassan AL-Griw, Zena A Zraba, Salsabiel K Al-Muntaser, Marwan M Draid, Aish M Zaidi, Refaat M Tabagh, Mohamed A AL-Griw (2017) Effects of storage temperature on the Quantity and Integrity of Genomic DNA Extracted from Mice Tissues: A Comparison of Recovery Methods. Open Veterinary Journal, 7 (3), 239-243. doi:10.4314/ovj.v7i3.7