E-ISSN 2218-6050 | ISSN 2226-4485
 

Short Communication


ADAPTATION OF THE AGYROPHIL TECHNIQUE FOR NUCLEOLAR ORGANIZER REGIONS TO CANINE PERIPHERAL BLOOD SMEARS

John Ogunsola, Richard Antia.


Cited By:1

Abstract
Nucleolar organizer regions (NORs) are chromosomal segments which are selectively stained by silver methods and evaluated as agyrophilic nucleolar organizer regions (AgNORs). The evaluation of AgNORs provides an insight into the level of cellular proliferation. This technique has extensively and sparsely been used in human and veterinary histopathology respectively. However, two major drawbacks have been irreproducible results and excessive staining precipitates. This study seeks to adapt the technique to canine peripheral blood smears in order to establish a routine staining protocol. Standardized volumes and concentrations of silver nitrate, gelatin, and formic acid were applied to smears at different temperatures for varying lengths of time. The technique was applied to unfixed and fixed smears. In some cases, a reducing agent (1% potassium iodide) was applied. It was shown that the optimum staining protocol was achieved by applying standardized solutions to a fixed smear at 46°C for 50 minutes. It is concluded that the staining protocol outlined in this study is practicable, and produces excellent and reproducible results that would enhance evaluation of AgNORs in canine peripheral blood cells.

Key words: AgNOR, canine blood smears, cytology, veterinary hematology


 
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How to Cite this Article
Pubmed Style

John Ogunsola, Richard Antia. ADAPTATION OF THE AGYROPHIL TECHNIQUE FOR NUCLEOLAR ORGANIZER REGIONS TO CANINE PERIPHERAL BLOOD SMEARS. Open Vet J. 2018; 8(2): 182-185. doi:10.4314/ovj.v8i2.11


Web Style

John Ogunsola, Richard Antia. ADAPTATION OF THE AGYROPHIL TECHNIQUE FOR NUCLEOLAR ORGANIZER REGIONS TO CANINE PERIPHERAL BLOOD SMEARS. https://www.openveterinaryjournal.com/?mno=281049 [Access: April 10, 2024]. doi:10.4314/ovj.v8i2.11


AMA (American Medical Association) Style

John Ogunsola, Richard Antia. ADAPTATION OF THE AGYROPHIL TECHNIQUE FOR NUCLEOLAR ORGANIZER REGIONS TO CANINE PERIPHERAL BLOOD SMEARS. Open Vet J. 2018; 8(2): 182-185. doi:10.4314/ovj.v8i2.11



Vancouver/ICMJE Style

John Ogunsola, Richard Antia. ADAPTATION OF THE AGYROPHIL TECHNIQUE FOR NUCLEOLAR ORGANIZER REGIONS TO CANINE PERIPHERAL BLOOD SMEARS. Open Vet J. (2018), [cited April 10, 2024]; 8(2): 182-185. doi:10.4314/ovj.v8i2.11



Harvard Style

John Ogunsola, Richard Antia (2018) ADAPTATION OF THE AGYROPHIL TECHNIQUE FOR NUCLEOLAR ORGANIZER REGIONS TO CANINE PERIPHERAL BLOOD SMEARS. Open Vet J, 8 (2), 182-185. doi:10.4314/ovj.v8i2.11



Turabian Style

John Ogunsola, Richard Antia. 2018. ADAPTATION OF THE AGYROPHIL TECHNIQUE FOR NUCLEOLAR ORGANIZER REGIONS TO CANINE PERIPHERAL BLOOD SMEARS. Open Veterinary Journal, 8 (2), 182-185. doi:10.4314/ovj.v8i2.11



Chicago Style

John Ogunsola, Richard Antia. "ADAPTATION OF THE AGYROPHIL TECHNIQUE FOR NUCLEOLAR ORGANIZER REGIONS TO CANINE PERIPHERAL BLOOD SMEARS." Open Veterinary Journal 8 (2018), 182-185. doi:10.4314/ovj.v8i2.11



MLA (The Modern Language Association) Style

John Ogunsola, Richard Antia. "ADAPTATION OF THE AGYROPHIL TECHNIQUE FOR NUCLEOLAR ORGANIZER REGIONS TO CANINE PERIPHERAL BLOOD SMEARS." Open Veterinary Journal 8.2 (2018), 182-185. Print. doi:10.4314/ovj.v8i2.11



APA (American Psychological Association) Style

John Ogunsola, Richard Antia (2018) ADAPTATION OF THE AGYROPHIL TECHNIQUE FOR NUCLEOLAR ORGANIZER REGIONS TO CANINE PERIPHERAL BLOOD SMEARS. Open Veterinary Journal, 8 (2), 182-185. doi:10.4314/ovj.v8i2.11