E-ISSN 2218-6050 | ISSN 2226-4485
 
2023, Vol: 13, Issue: 5
13/5Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Original Article


The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae

Marwa M. Attia, Marwa Khalifa, Olfat Mahdy.

Cited By:14

Abstract
Gasterophiline larvae are of veterinary and medical importance caused specific equine intestinal myiasis. Gasterophilus intestinalis (Botfly larvae) had a wide geographical distribution. The present study explores the prevalence rate of G. intestinalis 3rd stage larvae in Egypt from January- December 2017; besides, in vitro trials to control of this larvae and evaluation of this trial using Scanning Electron Microscope (SEM) and histopathology of treated larvae. In the present study, the 3rd larval stage of G. intestinalis was found in clusters in the epithelium of the investigated stomach and infested with prevalence rate 97.2%. The highest collected numbers of larvae were found in two months; March and August (570 & 520 larvae) and lowest numbers (200 larvae) were collected in October, November, and December. The calculated LC50 and LC90 values of neem seed extract were 707.9 ppm and 1090.7 ppm. The different alteration was recorded after exposure to oil extract which showed some destruction on cuticle surface as folded and corrugated cuticle, destruction of maxillae with pits on its surface, disfigure and irregularity of cephalic spines. Histopathology of exposed G. intestinalis larvae showed different changes as thinning of cuticle at the different level (exocuticle, endocuticle, cell layers), degeneration of epithelial cells of the gut and different degree of necrosis was described. Life cycle of G.intestinalis was followed up after treatment with neem seed extract.

Key words: Donkeys, Gasterophilus intestinalis , Larvicide, Neem seed extract, Prevalence, Egypt.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Marwa M. Attia
Articles by Marwa Khalifa
Articles by Olfat Mahdy
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Attia MM, Khalifa M, Mahdy O. The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Vet J. 2018; 8(4): 423-431. doi:10.4314/ovj.v8i4.12

Web Style

Attia MM, Khalifa M, Mahdy O. The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. https://www.openveterinaryjournal.com/?mno=297196 [Access: June 04, 2023]. doi:10.4314/ovj.v8i4.12

AMA (American Medical Association) Style

Attia MM, Khalifa M, Mahdy O. The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Vet J. 2018; 8(4): 423-431. doi:10.4314/ovj.v8i4.12


Vancouver/ICMJE Style

Attia MM, Khalifa M, Mahdy O. The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Vet J. (2018), [cited June 04, 2023]; 8(4): 423-431. doi:10.4314/ovj.v8i4.12


Harvard Style

Attia, M. M., Khalifa, . M. & Mahdy, . O. (2018) The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Vet J, 8 (4), 423-431. doi:10.4314/ovj.v8i4.12


Turabian Style

Attia, Marwa M., Marwa Khalifa, and Olfat Mahdy. 2018. The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Veterinary Journal, 8 (4), 423-431. doi:10.4314/ovj.v8i4.12


Chicago Style

Attia, Marwa M., Marwa Khalifa, and Olfat Mahdy. "The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae." Open Veterinary Journal 8 (2018), 423-431. doi:10.4314/ovj.v8i4.12


MLA (The Modern Language Association) Style

Attia, Marwa M., Marwa Khalifa, and Olfat Mahdy. "The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae." Open Veterinary Journal 8.4 (2018), 423-431. Print. doi:10.4314/ovj.v8i4.12


APA (American Psychological Association) Style

Attia, M. M., Khalifa, . M. & Mahdy, . O. (2018) The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Veterinary Journal, 8 (4), 423-431. doi:10.4314/ovj.v8i4.12


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information
This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the Budapest Open Access Initiative (BOAI) definition of open access.
The articles in Open Veterinary Journal are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Copyright © 2023 Open Veterinary Journal All Rights Reserved. Subject to change without notice from or liability to Open Veterinary Journal.
For best results, please use Internet Explorer or Google Chrome

Contact Information


All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal

Faculty of Veterinary Medicine

University of Tripoli

P.O. Box 13662, Tripoli, Libya