E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae

Marwa M. Attia, Marwa Khalifa, Olfat Mahdy.

Cited by (9)

Abstract
Gasterophiline larvae are of veterinary and medical importance caused specific equine intestinal myiasis. Gasterophilus intestinalis (Botfly larvae) had a wide geographical distribution. The present study explores the prevalence rate of G. intestinalis 3rd stage larvae in Egypt from January- December 2017; besides, in vitro trials to control of this larvae and evaluation of this trial using Scanning Electron Microscope (SEM) and histopathology of treated larvae. In the present study, the 3rd larval stage of G. intestinalis was found in clusters in the epithelium of the investigated stomach and infested with prevalence rate 97.2%. The highest collected numbers of larvae were found in two months; March and August (570 & 520 larvae) and lowest numbers (200 larvae) were collected in October, November, and December. The calculated LC50 and LC90 values of neem seed extract were 707.9 ppm and 1090.7 ppm. The different alteration was recorded after exposure to oil extract which showed some destruction on cuticle surface as folded and corrugated cuticle, destruction of maxillae with pits on its surface, disfigure and irregularity of cephalic spines. Histopathology of exposed G. intestinalis larvae showed different changes as thinning of cuticle at the different level (exocuticle, endocuticle, cell layers), degeneration of epithelial cells of the gut and different degree of necrosis was described. Life cycle of G.intestinalis was followed up after treatment with neem seed extract.

Key words: Donkeys, Gasterophilus intestinalis , Larvicide, Neem seed extract, Prevalence, Egypt.


 
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How to Cite this Article
Pubmed Style

Attia MM, Khalifa M, Mahdy O. The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Vet J. 2018; 8(4): 423-431. doi:10.4314/ovj.v8i4.12


Web Style

Attia MM, Khalifa M, Mahdy O. The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. https://www.openveterinaryjournal.com/?mno=297196 [Access: February 04, 2023]. doi:10.4314/ovj.v8i4.12


AMA (American Medical Association) Style

Attia MM, Khalifa M, Mahdy O. The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Vet J. 2018; 8(4): 423-431. doi:10.4314/ovj.v8i4.12



Vancouver/ICMJE Style

Attia MM, Khalifa M, Mahdy O. The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Vet J. (2018), [cited February 04, 2023]; 8(4): 423-431. doi:10.4314/ovj.v8i4.12



Harvard Style

Attia, M. M., Khalifa, . M. & Mahdy, . O. (2018) The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Vet J, 8 (4), 423-431. doi:10.4314/ovj.v8i4.12



Turabian Style

Attia, Marwa M., Marwa Khalifa, and Olfat Mahdy. 2018. The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Veterinary Journal, 8 (4), 423-431. doi:10.4314/ovj.v8i4.12



Chicago Style

Attia, Marwa M., Marwa Khalifa, and Olfat Mahdy. "The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae." Open Veterinary Journal 8 (2018), 423-431. doi:10.4314/ovj.v8i4.12



MLA (The Modern Language Association) Style

Attia, Marwa M., Marwa Khalifa, and Olfat Mahdy. "The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae." Open Veterinary Journal 8.4 (2018), 423-431. Print. doi:10.4314/ovj.v8i4.12



APA (American Psychological Association) Style

Attia, M. M., Khalifa, . M. & Mahdy, . O. (2018) The prevalence of Gasterophilus intestinalis (Diptera: Oestridae) in donkeys (Equus asinus) in Egypt with special reference to larvicidal effects of neem seed oil extract (Azadirachta indica) on third stage larvae. Open Veterinary Journal, 8 (4), 423-431. doi:10.4314/ovj.v8i4.12