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Original Research


Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study

M.I. Iswadi1, Z.F. Ann1, M.M. Hafiz2, M.D. Hafiz2, F.J. Fahrul2, H. Hajarian3, H. Wahid3, I. Zawawi4, M.S. Khairiah5 and O.A. Mazni6.


Abstract
The Malayan gaur (Bos gaurus hubbacki) or Seladang is classified as vulnerable by the International Union for Conservation of Nature and Natural Resources (IUCN). The Malayan gaur is mainly distributed in the tropical woodlands of Peninsular Malaysia and Southern Thailand. The aim of this study was to collect, analyze and cryopreserve the semen of wild Malayan gaur. Transrectal massage (TM) and electroejaculation (EEJ) technique was applied in semen collection of the Malayan gaur. The semen was then cryopreserved in liquid nitrogen using slow freezing technique. Makler counting chamber was used to evaluate sperm concentration and motility, while the sperm viability and morphology of fresh and post-thaw sperm was determined using eosin-nigrosin staining protocol. As a result, we have successfully collected the Malayan gaur semen using EEJ technique. Sperm motility, viability and morphological changes of the post-thaw semen of Malayan gaur were found undesirable due to the complication of the cryopreservation process. On the basis of current study it can be concluded that Malayan gaur bulls semen can be obtain by EEJ with no evidence of rectal trauma. Optimization of the process of cryopreservation for Malayan gaur sperm is needed to maintain the cryoviability of the good sperm quality. The data generated in this study would be useful in conservation of genetic diversity program for Malayan gaur.

Key words: Cryopreservation, Malayan gaur, Semen collection.


 
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How to Cite this Article
Pubmed Style

M.I. Iswadi, Z.F. Ann, M.M. Hafiz, M.D. Hafiz, F.J. Fahrul, H. Hajarian, H. Wahid, I. Zawawi, M.S. Khairiah and O.A. Mazni. Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study. Open Vet J. 2012; 2(0): 109-114. doi:10.5455/OVJ.2012.v2.i0.p109


Web Style

M.I. Iswadi, Z.F. Ann, M.M. Hafiz, M.D. Hafiz, F.J. Fahrul, H. Hajarian, H. Wahid, I. Zawawi, M.S. Khairiah and O.A. Mazni. Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study. https://www.openveterinaryjournal.com/?mno=302656797 [Access: June 03, 2024]. doi:10.5455/OVJ.2012.v2.i0.p109


AMA (American Medical Association) Style

M.I. Iswadi, Z.F. Ann, M.M. Hafiz, M.D. Hafiz, F.J. Fahrul, H. Hajarian, H. Wahid, I. Zawawi, M.S. Khairiah and O.A. Mazni. Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study. Open Vet J. 2012; 2(0): 109-114. doi:10.5455/OVJ.2012.v2.i0.p109



Vancouver/ICMJE Style

M.I. Iswadi, Z.F. Ann, M.M. Hafiz, M.D. Hafiz, F.J. Fahrul, H. Hajarian, H. Wahid, I. Zawawi, M.S. Khairiah and O.A. Mazni. Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study. Open Vet J. (2012), [cited June 03, 2024]; 2(0): 109-114. doi:10.5455/OVJ.2012.v2.i0.p109



Harvard Style

M.I. Iswadi, Z.F. Ann, M.M. Hafiz, M.D. Hafiz, F.J. Fahrul, H. Hajarian, H. Wahid, I. Zawawi, M.S. Khairiah and O.A. Mazni (2012) Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study. Open Vet J, 2 (0), 109-114. doi:10.5455/OVJ.2012.v2.i0.p109



Turabian Style

M.I. Iswadi, Z.F. Ann, M.M. Hafiz, M.D. Hafiz, F.J. Fahrul, H. Hajarian, H. Wahid, I. Zawawi, M.S. Khairiah and O.A. Mazni. 2012. Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study. Open Veterinary Journal, 2 (0), 109-114. doi:10.5455/OVJ.2012.v2.i0.p109



Chicago Style

M.I. Iswadi, Z.F. Ann, M.M. Hafiz, M.D. Hafiz, F.J. Fahrul, H. Hajarian, H. Wahid, I. Zawawi, M.S. Khairiah and O.A. Mazni. "Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study." Open Veterinary Journal 2 (2012), 109-114. doi:10.5455/OVJ.2012.v2.i0.p109



MLA (The Modern Language Association) Style

M.I. Iswadi, Z.F. Ann, M.M. Hafiz, M.D. Hafiz, F.J. Fahrul, H. Hajarian, H. Wahid, I. Zawawi, M.S. Khairiah and O.A. Mazni. "Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study." Open Veterinary Journal 2.0 (2012), 109-114. Print. doi:10.5455/OVJ.2012.v2.i0.p109



APA (American Psychological Association) Style

M.I. Iswadi, Z.F. Ann, M.M. Hafiz, M.D. Hafiz, F.J. Fahrul, H. Hajarian, H. Wahid, I. Zawawi, M.S. Khairiah and O.A. Mazni (2012) Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study. Open Veterinary Journal, 2 (0), 109-114. doi:10.5455/OVJ.2012.v2.i0.p109