E-ISSN 2218-6050 | ISSN 2226-4485
 

-


Microscopic analysis of MTT stained boar sperm cells

B.M. van den Berg*.


Abstract
The ability of sperm cells to develop colored formazan by reduction of MTT was used earlier to develop a spectrophotometric assay to determine the viability of sperm cells for several mammalian species. It was the objective of the present study to visualize microscopically the location of the formazan in boar sperm cells. The MTT staining process of boar sperm cells can be divided into a series of morphological events. Incubation of the sperm cells in the presence of MTT resulted after a few min in a diffuse staining of the midpiece of the sperm cells. Upon further incubation the staining of the midpiece became more intense, and gradually the formation of packed formazan granules became more visible. At the same time, a small formazan stained granule appeared medially on the sperm head, which increased in size during further incubation. After incubation for about 1 h the midpiece granules were intensely stained and more clearly distinct as granules, while aggregation of sperm cells occurred. Around 90% of the sperm cells showed these staining events. At the end of the staining the formazan granules have disappeared from both the sperm cells and medium, whereas formazan crystals appeared as thin crystal threads, that became heavily aggregated in the incubation medium. It was concluded that formazan is taken up by lipid droplets in the cytoplasm. Further, the use of the MTT assay to test for sperm viability should be regarded as a qualitative assay, whereas its practical use at artificial insemination (AI) Stations is limited.

Key words: Boar, MTT staining, Sperm, Viability.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by B.M. van den Berg*
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

B.M. van den Berg. Microscopic analysis of MTT stained boar sperm cells. Open Vet J. 2015; 5(1): 58-63. doi:10.5455/OVJ.2015.v5.i1.p58


Web Style

B.M. van den Berg. Microscopic analysis of MTT stained boar sperm cells. https://www.openveterinaryjournal.com/?mno=302656824 [Access: June 04, 2024]. doi:10.5455/OVJ.2015.v5.i1.p58


AMA (American Medical Association) Style

B.M. van den Berg. Microscopic analysis of MTT stained boar sperm cells. Open Vet J. 2015; 5(1): 58-63. doi:10.5455/OVJ.2015.v5.i1.p58



Vancouver/ICMJE Style

B.M. van den Berg. Microscopic analysis of MTT stained boar sperm cells. Open Vet J. (2015), [cited June 04, 2024]; 5(1): 58-63. doi:10.5455/OVJ.2015.v5.i1.p58



Harvard Style

B.M. van den Berg (2015) Microscopic analysis of MTT stained boar sperm cells. Open Vet J, 5 (1), 58-63. doi:10.5455/OVJ.2015.v5.i1.p58



Turabian Style

B.M. van den Berg. 2015. Microscopic analysis of MTT stained boar sperm cells. Open Veterinary Journal, 5 (1), 58-63. doi:10.5455/OVJ.2015.v5.i1.p58



Chicago Style

B.M. van den Berg. "Microscopic analysis of MTT stained boar sperm cells." Open Veterinary Journal 5 (2015), 58-63. doi:10.5455/OVJ.2015.v5.i1.p58



MLA (The Modern Language Association) Style

B.M. van den Berg. "Microscopic analysis of MTT stained boar sperm cells." Open Veterinary Journal 5.1 (2015), 58-63. Print. doi:10.5455/OVJ.2015.v5.i1.p58



APA (American Psychological Association) Style

B.M. van den Berg (2015) Microscopic analysis of MTT stained boar sperm cells. Open Veterinary Journal, 5 (1), 58-63. doi:10.5455/OVJ.2015.v5.i1.p58