E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 11
14 / 11Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

-


Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena

P.N. Agbedanu1,2, M.T. Brewer1,2, T.A. Day2, M.J. Kimber2, K.L. Anderson2, S.K. Rasmussen3, M.A. Rasmussen4 and S.A. Carlson2,*.


Cited By:1

Abstract
In an effort to investigate the molecular basis of protozoa engulfment-mediated hypervirulence of Salmonella in cattle, we evaluated protozoan G protein-coupled receptors (GPCRs) as transducers of Salmonella engulfment by the model protozoan Tetrahymena. Our laboratory previously demonstrated that non-pathogenic protozoa (including Tetrahymena) engulf Salmonella and then exacerbate its virulence in cattle, but the mechanistic details of the phenomenon are not fully understood. GPCRs were investigated since these receptors facilitate phagocytosis of particulates by Tetrahymena, and a GPCR apparently modulates bacterial engulfment for the pathogenic protozoan Entamoeba histolytica. A database search identified three putative Tetrahymena GPCRs, based on sequence homologies and predicted transmembrane domains, that were the focus of this study. Salmonella engulfment by Tetrahymena was assessed in the presence of suramin, a non-specific GPCR inhibitor. Salmonella engulfment was also assessed in Tetrahymena in which expression of putative GPCRs was knocked-down using RNAi. A candidate GPCR was then expressed in a heterologous yeast expression system for further characterization. Our results revealed that Tetrahymena were less efficient at engulfing Salmonella in the presence of suramin. Engulfment was reduced concordantly with a reduction in the density of protozoa. RNAi-based studies revealed that knock-down of one the Tetrahymena GPCRs caused diminished engulfment of Salmonella. Tetrahymena lysates activated this receptor in the heterologous expression system. These data demonstrate that the Tetrahymena receptor is a putative GPCR that facilitates bacterial engulfment by Tetrahymena. Activation of the putative GPCR seemed to be related to protozoan cell density, suggesting that its cognate ligand is an intercellular signaling molecule.

Key words: GPCR, Protozoa, Salmonella, Virulence.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by P.N. Agbedanu1
Articles by 2
Articles by M.T. Brewer1
Articles by 2
Articles by T.A. Day2
Articles by M.J. Kimber2
Articles by K.L. Anderson2
Articles by S.K. Rasmussen3
Articles by M.A. Rasmussen4 and S.A. Carlson2
Articles by *
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

P.N. Agbedanu,, M.T. Brewer,, T.A. Day, M.J. Kimber, K.L. Anderson, S.K. Rasmussen, M.A. Rasmussen and S.A. Carlson,. Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena. Open Vet J. 2013; 3(2): 69-74. doi:10.5455/OVJ.2013.v3.i2.p69

Web Style

P.N. Agbedanu,, M.T. Brewer,, T.A. Day, M.J. Kimber, K.L. Anderson, S.K. Rasmussen, M.A. Rasmussen and S.A. Carlson,. Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena. https://www.openveterinaryjournal.com/?mno=302656852 [Access: December 07, 2024]. doi:10.5455/OVJ.2013.v3.i2.p69

AMA (American Medical Association) Style

P.N. Agbedanu,, M.T. Brewer,, T.A. Day, M.J. Kimber, K.L. Anderson, S.K. Rasmussen, M.A. Rasmussen and S.A. Carlson,. Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena. Open Vet J. 2013; 3(2): 69-74. doi:10.5455/OVJ.2013.v3.i2.p69


Vancouver/ICMJE Style

P.N. Agbedanu,, M.T. Brewer,, T.A. Day, M.J. Kimber, K.L. Anderson, S.K. Rasmussen, M.A. Rasmussen and S.A. Carlson,. Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena. Open Vet J. (2013), [cited December 07, 2024]; 3(2): 69-74. doi:10.5455/OVJ.2013.v3.i2.p69


Harvard Style

P.N. Agbedanu,, M.T. Brewer,, T.A. Day, M.J. Kimber, K.L. Anderson, S.K. Rasmussen, M.A. Rasmussen and S.A. Carlson, (2013) Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena. Open Vet J, 3 (2), 69-74. doi:10.5455/OVJ.2013.v3.i2.p69


Turabian Style

P.N. Agbedanu,, M.T. Brewer,, T.A. Day, M.J. Kimber, K.L. Anderson, S.K. Rasmussen, M.A. Rasmussen and S.A. Carlson,. 2013. Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena. Open Veterinary Journal, 3 (2), 69-74. doi:10.5455/OVJ.2013.v3.i2.p69


Chicago Style

P.N. Agbedanu,, M.T. Brewer,, T.A. Day, M.J. Kimber, K.L. Anderson, S.K. Rasmussen, M.A. Rasmussen and S.A. Carlson,. "Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena." Open Veterinary Journal 3 (2013), 69-74. doi:10.5455/OVJ.2013.v3.i2.p69


MLA (The Modern Language Association) Style

P.N. Agbedanu,, M.T. Brewer,, T.A. Day, M.J. Kimber, K.L. Anderson, S.K. Rasmussen, M.A. Rasmussen and S.A. Carlson,. "Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena." Open Veterinary Journal 3.2 (2013), 69-74. Print. doi:10.5455/OVJ.2013.v3.i2.p69


APA (American Psychological Association) Style

P.N. Agbedanu,, M.T. Brewer,, T.A. Day, M.J. Kimber, K.L. Anderson, S.K. Rasmussen, M.A. Rasmussen and S.A. Carlson, (2013) Involvement of a putative intercellular signal-recognizing G protein-coupled receptor in the engulfment of Salmonella by the protozoan Tetrahymena. Open Veterinary Journal, 3 (2), 69-74. doi:10.5455/OVJ.2013.v3.i2.p69


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly