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Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood

H.A. Hussein* and A.A. Aamer.


Cited By:17

Abstract
The present study was designed to investigate the effects of storage temperature and time on blood gas and acid-base balance of ovine venous blood. Ten clinically healthy sheep were used in this study. A total number of 30 blood samples, were divided into three different groups, and were stored in a refrigerator adjusted to +4 ºC (Group I, n = 10), at RT of about 22-25 ºC (Group II, n = 10) and in an incubator adjusted to 37 ºC (Group III, n = 10) for up to 48 h. Blood samples were analysed for blood gas and acid-base indices at 0, 1, 2, 3, 4, 5, 6, 12, 24 and 48 h of storage. In comparison to the baseline value (0), there were significant decreases of blood pH of samples stored at RT and in the incubator after 1 h (p<0.05), the pH value of refrigerated blood samples exhibited insignificant changes during the study (p<0.05). Mean values of pCO2 showed a significant increase in Group I and Group III after 1 h then a progressive decrease after 12 h in all Groups. Mean pO2 values were significantly higher for Group I after 2 h and for Groups II and III after 1 h (p<0.05). In general, base excess decreased significantly for all the groups during the study especially in Groups II and III. In comparison with baseline values, in all groups, bicarbonate (HCO3) increased between 1 h and 6 h (p<0.05), and later decreased at the end of the study (p<0.05). In conclusion, status of acid-base indices of the samples stored at refrigerator and RT were found within normal reference range and it may be of clinical diagnostic use for up to 6 h.

Key words: Acid-base indices, Clinical relevance, Ovine, Storage temperature time.


 
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How to Cite this Article
Pubmed Style

H.A. Hussein and A.A. Aamer. Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood. Open Vet J. 2013; 3(1): 1-7. doi:10.5455/OVJ.2013.v3.i1.p1


Web Style

H.A. Hussein and A.A. Aamer. Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood. https://www.openveterinaryjournal.com/?mno=302656867 [Access: February 28, 2024]. doi:10.5455/OVJ.2013.v3.i1.p1


AMA (American Medical Association) Style

H.A. Hussein and A.A. Aamer. Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood. Open Vet J. 2013; 3(1): 1-7. doi:10.5455/OVJ.2013.v3.i1.p1



Vancouver/ICMJE Style

H.A. Hussein and A.A. Aamer. Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood. Open Vet J. (2013), [cited February 28, 2024]; 3(1): 1-7. doi:10.5455/OVJ.2013.v3.i1.p1



Harvard Style

H.A. Hussein and A.A. Aamer (2013) Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood. Open Vet J, 3 (1), 1-7. doi:10.5455/OVJ.2013.v3.i1.p1



Turabian Style

H.A. Hussein and A.A. Aamer. 2013. Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood. Open Veterinary Journal, 3 (1), 1-7. doi:10.5455/OVJ.2013.v3.i1.p1



Chicago Style

H.A. Hussein and A.A. Aamer. "Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood." Open Veterinary Journal 3 (2013), 1-7. doi:10.5455/OVJ.2013.v3.i1.p1



MLA (The Modern Language Association) Style

H.A. Hussein and A.A. Aamer. "Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood." Open Veterinary Journal 3.1 (2013), 1-7. Print. doi:10.5455/OVJ.2013.v3.i1.p1



APA (American Psychological Association) Style

H.A. Hussein and A.A. Aamer (2013) Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood. Open Veterinary Journal, 3 (1), 1-7. doi:10.5455/OVJ.2013.v3.i1.p1