E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine Staphylococcus aureus isolates

Sarhan R Sarhan, Hyder O Hashim, Mohammed Baqur S Al Shuhaib.


Cited By:19

Abstract
This study was conducted to detect the mecA gene polymorphism in ovine wounds and its possible association with the structure and function of penicillin binding protein A2 (PBP2A). One genetic locus of 1967 bp that covered the majority of the coding regions of the mecA gene within methicillin-resistant Staphylococcus aureus (MRSA) DNA sequences was designed. In addition to standard microbiological tests, PCR-sequencing reactions and phylogenetic analyses confirmed the identity of the targeted MRSA bacteria. Seven novel missense SNPs, including N57T, N115Y, D120N, D139N, G152V, E189K, and F211V, were observed in the mecA amplicons. Multiple state-of-the-art in silico tools were utilized to assess the consequences of each observed SNP in terms of its effect on the corresponding PBP2A protein structure and function. It was shown that some MRSA isolates exhibited a highly PBP2A-damaging SNP, G152V, which showed an entirely deleterious effect on the PBP2A. Furthermore, G152V induced an alteration in the PBP2A interaction with its receptor, which presumably reduced its affinity to bind with the beta-lactams. In conclusion, the present report indicated a possible role for the observed deleterious G152V SNP in the reduction of PBP2A binding with beta-lactams, which has led to a remarkable increase in MRSA’s resistance to antibiotics.

Key words: Infection, In silico, MecA, Missense, Variations


 
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How to Cite this Article
Pubmed Style

Sarhan SR, Hashim HO, Shuhaib MBSA, . The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine Staphylococcus aureus isolates. Open Vet J. 2019; 9(4): 339-348. doi:10.4314/ovj.v9i4.12


Web Style

Sarhan SR, Hashim HO, Shuhaib MBSA, . The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine Staphylococcus aureus isolates. https://www.openveterinaryjournal.com/?mno=50203 [Access: February 28, 2024]. doi:10.4314/ovj.v9i4.12


AMA (American Medical Association) Style

Sarhan SR, Hashim HO, Shuhaib MBSA, . The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine Staphylococcus aureus isolates. Open Vet J. 2019; 9(4): 339-348. doi:10.4314/ovj.v9i4.12



Vancouver/ICMJE Style

Sarhan SR, Hashim HO, Shuhaib MBSA, . The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine Staphylococcus aureus isolates. Open Vet J. (2019), [cited February 28, 2024]; 9(4): 339-348. doi:10.4314/ovj.v9i4.12



Harvard Style

Sarhan, S. R., Hashim, H. O., Shuhaib, M. B. S. A. & (2019) The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine Staphylococcus aureus isolates. Open Vet J, 9 (4), 339-348. doi:10.4314/ovj.v9i4.12



Turabian Style

Sarhan, Sarhan R, Hyder O Hashim, Mohammed Baqur S Al Shuhaib, and . 2019. The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine Staphylococcus aureus isolates. Open Veterinary Journal, 9 (4), 339-348. doi:10.4314/ovj.v9i4.12



Chicago Style

Sarhan, Sarhan R, Hyder O Hashim, Mohammed Baqur S Al Shuhaib, and . "The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine Staphylococcus aureus isolates." Open Veterinary Journal 9 (2019), 339-348. doi:10.4314/ovj.v9i4.12



MLA (The Modern Language Association) Style

Sarhan, Sarhan R, Hyder O Hashim, Mohammed Baqur S Al Shuhaib, and . "The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine Staphylococcus aureus isolates." Open Veterinary Journal 9.4 (2019), 339-348. Print. doi:10.4314/ovj.v9i4.12



APA (American Psychological Association) Style

Sarhan, S. R., Hashim, H. O., Shuhaib, M. B. S. A. & (2019) The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine Staphylococcus aureus isolates. Open Veterinary Journal, 9 (4), 339-348. doi:10.4314/ovj.v9i4.12