E-ISSN 2218-6050 | ISSN 2226-4485
 

Review Article


The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols

Kakanang Buranaamnuay.

Cited by (10)

Abstract
The MTT reduction assay is one of the methods used to evaluate the viability of sperm. In the assay, a tetrazolium component (MTT) is converted into MTT formazan by some specific enzymes in the viable cells. The amount of formazan product which is in theory directly correlated with the percentage of viable sperm is quantified by measuring the absorbance using a spectrophotometer. The present article compiles the MTT assays that have been used to determine sperm viability in most animal species and human. In each assay, other factors apart from the number of viable cells which potentially influence the accuracy and precision of results are stated, for instance, preparations of sperm and MTT solutions, length and conditions of incubation, a solubilizing agent as well as the formazan detection method. Also, strengths and shortcomings of the MTT test compared with others are summarized at the end of this article. This information may be useful for prospective researchers who are deciding to implement this colorimetric method in their experiments.

Key words: Colorimetric assay, Metabolic activity, Spectrophotometer, Sperm


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Kakanang Buranaamnuay
on Google
on Google Scholar


This Article Cited By the following articles

Supplementation of melatonin to cooling and freezing extenders improves canine spermatozoa quality measures
BMC Vet Res 2022; 18(1): .

1
 
Drug toxicity assessment: cell proliferation versus cell death
Cell Death Discov. 2022; 8(1): .

2
 
Anticancer Potential of Hesperidin against HEp-2 Laryngeal Carcinoma Cell Line in Comparison to Doxorubicin
Open Access Maced J Med Sci 2022; 10(A): 1300.

3
 
Accumulated oxidative stress risk in HUVECs by chronic exposure to non-observable acute effect levels of PM2.5
Toxicology in Vitro 2022; 82(): 105376.

4
 
Effects of epididymal sperm recovery methods on fresh and frozen–thawed sperm characteristics in dogs
Reprod Domestic Animals 2022; 57(9): 1038.

5
 
Ex vivo exposures to arsenite and its methylated trivalent metabolites alter gene transcription in mouse sperm cells
Toxicology and Applied Pharmacology 2022; 455(): 116266.

6
 
Short cytoplasmic isoform of IL1R1/CD121a mediates IL1β induced proliferation of synovium-derived mesenchymal stem/stromal cells through ERK1/2 pathway
Heliyon 2022; 8(5): e09476.

7
 
The effects of green synthesized anionic cupric oxide nanoparticles on Zaraibi goat spermatozoa during cryopreservation with and without removal of seminal plasma
Animal Biotechnology 2022; (): 1.

8
 
Optimizing component formula suppresses lung cancer by blocking DTL-mediated PDCD4 ubiquitination to regulate the MAPK/JNK pathway
Journal of Ethnopharmacology 2022; 299(): 115546.

9
 
Different Seasonal Collections of Ficus carica L. Leaves Diversely Modulate Lipid Metabolism and Adipogenesis in 3T3-L1 Adipocytes
Nutrients 2022; 14(14): 2833.

10
 
How to Cite this Article
Pubmed Style

Buranaamnuay K, . The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols. Open Vet J. 2021; 11(2): 251-269. doi:10.5455/OVJ.2021.v11.i2.9


Web Style

Buranaamnuay K, . The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols. https://www.openveterinaryjournal.com/?mno=50208 [Access: November 28, 2022]. doi:10.5455/OVJ.2021.v11.i2.9


AMA (American Medical Association) Style

Buranaamnuay K, . The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols. Open Vet J. 2021; 11(2): 251-269. doi:10.5455/OVJ.2021.v11.i2.9



Vancouver/ICMJE Style

Buranaamnuay K, . The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols. Open Vet J. (2021), [cited November 28, 2022]; 11(2): 251-269. doi:10.5455/OVJ.2021.v11.i2.9



Harvard Style

Buranaamnuay, K. & (2021) The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols. Open Vet J, 11 (2), 251-269. doi:10.5455/OVJ.2021.v11.i2.9



Turabian Style

Buranaamnuay, Kakanang, and . 2021. The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols. Open Veterinary Journal, 11 (2), 251-269. doi:10.5455/OVJ.2021.v11.i2.9



Chicago Style

Buranaamnuay, Kakanang, and . "The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols." Open Veterinary Journal 11 (2021), 251-269. doi:10.5455/OVJ.2021.v11.i2.9



MLA (The Modern Language Association) Style

Buranaamnuay, Kakanang, and . "The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols." Open Veterinary Journal 11.2 (2021), 251-269. Print. doi:10.5455/OVJ.2021.v11.i2.9



APA (American Psychological Association) Style

Buranaamnuay, K. & (2021) The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols. Open Veterinary Journal, 11 (2), 251-269. doi:10.5455/OVJ.2021.v11.i2.9