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The potential anti- African swine fever virus effects of medium chain fatty acids on in vitro feed model: An evaluation study using a field ASFV strain isolated in Vietnam

Ha Thi Thanh Tran, Duc Anh Truong, Viet Duc Ly, Tuan Van Hoang, Nhu Thi Chu, Huyen Thi Nguyen, Anh Thi Kieu Dang, Maartje De Vos, Kobe Lannoo, Geert Bruggeman, Hoang Vu Dang.


Cited By:6

Abstract
Background: African swine fever (ASF) is an important disease affecting swine and has a significant economic loss on both the developed and developing world. Previous studies have indicated the mitigation of African swine fever virus (ASFV) in feed, suggesting that the risk of ASFV transmissibility via the oral exposure.
Aim: We evaluated the potential effects of MCFAs in both individual and synergistic forms, to prevent and/or reduce ASFV infection using in vitro feed model.
Methods: The cytotoxicity of MCFAs on PAM cells were evaluated by using the MTT assay. The potential effects of MCFAs, including C8, C6-C8-C10 (1:1:1 ratio) and C8-C10-C12 (1:1:1 ratio) against a field ASFV strain isolated in the Capital Hanoi of Vietnam were further examined by real-time PCR and HAD assays in in vitro feed model.
Results: Our results indicated that all products do not induce the cytotoxicity at the dose of 100 µg/ml and are suitable for further in vitro examination. All tested products have shown a strong antiviral effect against ASFV infectivity at doses of 0.375% and 0.5%. Interestingly, the synergistic MCFAs have shown clearly their potential activities against ASFV in which at lower dose of 0.25%, pre-treatment with product 2 and 3 induced significant increases at the level of Cq value when compared to positive control and/or product 1 (P<0.05). However, the viral titre was not changed after 24h post-inoculation when compared to positive control by HAD assay.
Conclusion: Our findings suggested that all tested products, both individual and synergistic forms of MCFAs, have possessed a strong anti-ASFV effect and this effect is dose- dependence in in vitro feed model. Additionally, synergistic effects of MCFAs are more effective against ASFV when compared to individual form. The further studies focusing on in vivo anti-ASFV effects of MCFAs are important to bring new insight into the mode of ASFV-reduced action by these compounds in swine feed.

Key words: African swine fever virus; virus isolation; MCFA; pig; Vietnam


 
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How to Cite this Article
Pubmed Style

Tran HTT, Truong DA, Ly VD, Hoang TV, Chu NT, Nguyen HT, Dang ATK, Vos MD, Lannoo K, Bruggeman G, Dang HV, . The potential anti- African swine fever virus effects of medium chain fatty acids on in vitro feed model: An evaluation study using a field ASFV strain isolated in Vietnam. Open Vet J. 2021; 11(3): 346-355. doi:10.5455/OVJ.2021.v11.i3.3

Web Style

Tran HTT, Truong DA, Ly VD, Hoang TV, Chu NT, Nguyen HT, Dang ATK, Vos MD, Lannoo K, Bruggeman G, Dang HV, . The potential anti- African swine fever virus effects of medium chain fatty acids on in vitro feed model: An evaluation study using a field ASFV strain isolated in Vietnam. https://www.openveterinaryjournal.com/?mno=79280 [Access: December 07, 2024]. doi:10.5455/OVJ.2021.v11.i3.3

AMA (American Medical Association) Style

Tran HTT, Truong DA, Ly VD, Hoang TV, Chu NT, Nguyen HT, Dang ATK, Vos MD, Lannoo K, Bruggeman G, Dang HV, . The potential anti- African swine fever virus effects of medium chain fatty acids on in vitro feed model: An evaluation study using a field ASFV strain isolated in Vietnam. Open Vet J. 2021; 11(3): 346-355. doi:10.5455/OVJ.2021.v11.i3.3


Vancouver/ICMJE Style

Tran HTT, Truong DA, Ly VD, Hoang TV, Chu NT, Nguyen HT, Dang ATK, Vos MD, Lannoo K, Bruggeman G, Dang HV, . The potential anti- African swine fever virus effects of medium chain fatty acids on in vitro feed model: An evaluation study using a field ASFV strain isolated in Vietnam. Open Vet J. (2021), [cited December 07, 2024]; 11(3): 346-355. doi:10.5455/OVJ.2021.v11.i3.3


Harvard Style

Tran, H. T. T., Truong, D. A., Ly, V. D., Hoang, T. V., Chu, N. T., Nguyen, H. T., Dang, A. T. K., Vos, M. D., Lannoo, K., Bruggeman, G., Dang, H. V. & (2021) The potential anti- African swine fever virus effects of medium chain fatty acids on in vitro feed model: An evaluation study using a field ASFV strain isolated in Vietnam. Open Vet J, 11 (3), 346-355. doi:10.5455/OVJ.2021.v11.i3.3


Turabian Style

Tran, Ha Thi Thanh, Duc Anh Truong, Viet Duc Ly, Tuan Van Hoang, Nhu Thi Chu, Huyen Thi Nguyen, Anh Thi Kieu Dang, Maartje De Vos, Kobe Lannoo, Geert Bruggeman, Hoang Vu Dang, and . 2021. The potential anti- African swine fever virus effects of medium chain fatty acids on in vitro feed model: An evaluation study using a field ASFV strain isolated in Vietnam. Open Veterinary Journal, 11 (3), 346-355. doi:10.5455/OVJ.2021.v11.i3.3


Chicago Style

Tran, Ha Thi Thanh, Duc Anh Truong, Viet Duc Ly, Tuan Van Hoang, Nhu Thi Chu, Huyen Thi Nguyen, Anh Thi Kieu Dang, Maartje De Vos, Kobe Lannoo, Geert Bruggeman, Hoang Vu Dang, and . "The potential anti- African swine fever virus effects of medium chain fatty acids on in vitro feed model: An evaluation study using a field ASFV strain isolated in Vietnam." Open Veterinary Journal 11 (2021), 346-355. doi:10.5455/OVJ.2021.v11.i3.3


MLA (The Modern Language Association) Style

Tran, Ha Thi Thanh, Duc Anh Truong, Viet Duc Ly, Tuan Van Hoang, Nhu Thi Chu, Huyen Thi Nguyen, Anh Thi Kieu Dang, Maartje De Vos, Kobe Lannoo, Geert Bruggeman, Hoang Vu Dang, and . "The potential anti- African swine fever virus effects of medium chain fatty acids on in vitro feed model: An evaluation study using a field ASFV strain isolated in Vietnam." Open Veterinary Journal 11.3 (2021), 346-355. Print. doi:10.5455/OVJ.2021.v11.i3.3


APA (American Psychological Association) Style

Tran, H. T. T., Truong, D. A., Ly, V. D., Hoang, T. V., Chu, N. T., Nguyen, H. T., Dang, A. T. K., Vos, M. D., Lannoo, K., Bruggeman, G., Dang, H. V. & (2021) The potential anti- African swine fever virus effects of medium chain fatty acids on in vitro feed model: An evaluation study using a field ASFV strain isolated in Vietnam. Open Veterinary Journal, 11 (3), 346-355. doi:10.5455/OVJ.2021.v11.i3.3


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