Abstract
Foot-and-mouth disease (FMD) is an endemic disease of clove hoofed animals in Bangladesh and multiple outbreaks occurred every year caused by FMD virus (FMDV). The aim of the present investigation was to molecular characterization of VP1 coding region of FMDV serotype O outbreak in cattle. A total of 4 tongue epithelial specimens were collected from clinically FMD positive cattle during June 2018 in Manikgonj district of Bangladesh. All four isolates were recorded positive for FMDV serotype O. The phylogenetic analysis showed that two isolates were clustered within an emerging novel sublineage Ind2001BD1 under lineage Ind2001 of FMDV serotype O, which was identified during 2012-2016 in Bangladesh. One isolate was clustered within lineage PanAsia of FMDV serotype O and closely related with isolate identified in Nepal in 2009. The phylogenetic reconstruction revealed that all four isolates belong to the Middle East–South Asia (ME–SA) topotype. Therefore, multiple lineages of FMDV serotype O are circulating in cattle in the outbreak area that makes it more complex for the FMD control program in Bangladesh. A comprehensive study on the genetic characteristics of FMDV over the country is required for effective FMD prevention and control strategy.

Key words: Cattle, lineage, Ind2001BD1, PanAsia, Foot-and-mouth disease