Abstract
Background:
Hypercholesterolemia is frequently linked to an elevated risk of cardiovascular diseases, including heart attacks and strokes. Additionally, it could be connected to a higher susceptibility to osteoporosis. Hypercholesterolemia can stimulate differentiation and activity of osteoclasts, leading to enhanced bone reabsorption and a subsequent net loss of bone tissue.
Aim:
The purpose of this study was to examine the influence of a high cholesterol diet on osteoporosis in male rats with differences in biological and oxidative indicators in hypercholesterolemia diet in male rat.
Method:
The samples in this study were twenty male rats, ranging between 1.5 and 2 months, were separated into two groups. In one group, 10 rats were fed a regular diet, while in another group, 10 rats were fed a high cholesterol diet (2%) over the course of 8 weeks. Samples of blood were obtained at the last stage of the experiment. to calculate physiological and biological markers including ERK, TRAP, hormones, MDA, and GSH.
Results:
The results of this study demonstrated a decrease in glutathione levels, an increase in extracellular signal-regulated kinase levels (ERK), no significant change in serum TRAP levels, an increase in Malondialdehyde levels in the blood, and elevated levels of parathyroid hormone, Calcitonin, and Vitamin D in the cholesterol group.
Conclusion:
An increased oxidative stress, altered signaling, and disruptions in calcium/bone metabolism associated with cholesterol-related condition and monitoring biomarker ERK can provide valuable information about disease progression.

Key words: Hypercholesterolemia, Osteoporosis, ERK, TRAP