E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 10
14 / 10Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Research Article


Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent

Suraida Meisari, Tita Damayanti Lestari, Djoko Legowo, Thomas Valentinus Widiyatno, Eka Pramyrtha Hestianah, Retno Sri Wahjuni, Ratna Damayanti, Aswin Rafif Khairullah, Ikechukwu Benjamin Moses, Ricadonna Raissa.


Abstract
Background:
Cases of cruelty can occur in wild animal, livestock, and pet animal. Cruelty to cats choose to be the background of this research because many cases of cruelty to cats have not reported although the cases are still high.

Aim:
Aim of this research was to know how much dilution of cat blood that can still be detected by Leucomalachite Green (LMG) and Takayama reagent.

Methods:
Three samples of domestic cat blood were diluted with ratio 1:10; 1:100; 1:1,000; 1:10,000; and 1:100,000. Bloodstain is made by dropping each blood dilution on the filter paper for LMG test and on the object glass for Takayama test. Bloodstain for LMG test was done with ten repetitions from each sample and duplo for Takayama test. Positive results from the LMG test are presented as a bluish-green discoloration in stains. A positive result from the Takayama test is the formation of hemochromogen crystals under a microscope with 400x magnification.

Results:
Based on this research, cat bloodstain can be detected with LMG reagent until 1:40,000 dilution, while Takayama reagent only can detect cat bloodstain until 1:1,000.

Conclusion:
LMG and Takayama reagent are reagents that are often used in human blood spot testing. If there is a case of violence against cats and other animals, these two reagents can be relied on to help with the proof process.

Key words: Bloodstain, Cat, Leucomalachite Green, RGB value, Takayama


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Suraida Meisari
Articles by Tita Damayanti Lestari
Articles by Djoko Legowo
Articles by Thomas Valentinus Widiyatno
Articles by Eka Pramyrtha Hestianah
Articles by Retno Sri Wahjuni
Articles by Ratna Damayanti
Articles by Aswin Rafif Khairullah
Articles by Ikechukwu Benjamin Moses
Articles by Ricadonna Raissa
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Meisari S, Lestari TD, Legowo D, Widiyatno TV, Hestianah EP, Wahjuni RS, Damayanti R, Khairullah AR, Moses IB, Raissa R. Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent. Open Vet J. 2024; 14(10): 2609-2617. doi:10.5455/OVJ.2024.v14.i10.10

Web Style

Meisari S, Lestari TD, Legowo D, Widiyatno TV, Hestianah EP, Wahjuni RS, Damayanti R, Khairullah AR, Moses IB, Raissa R. Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent. https://www.openveterinaryjournal.com/?mno=207656 [Access: October 30, 2024]. doi:10.5455/OVJ.2024.v14.i10.10

AMA (American Medical Association) Style

Meisari S, Lestari TD, Legowo D, Widiyatno TV, Hestianah EP, Wahjuni RS, Damayanti R, Khairullah AR, Moses IB, Raissa R. Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent. Open Vet J. 2024; 14(10): 2609-2617. doi:10.5455/OVJ.2024.v14.i10.10


Vancouver/ICMJE Style

Meisari S, Lestari TD, Legowo D, Widiyatno TV, Hestianah EP, Wahjuni RS, Damayanti R, Khairullah AR, Moses IB, Raissa R. Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent. Open Vet J. (2024), [cited October 30, 2024]; 14(10): 2609-2617. doi:10.5455/OVJ.2024.v14.i10.10


Harvard Style

Meisari, S., Lestari, . T. D., Legowo, . D., Widiyatno, . T. V., Hestianah, . E. P., Wahjuni, . R. S., Damayanti, . R., Khairullah, . A. R., Moses, . I. B. & Raissa, . R. (2024) Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent. Open Vet J, 14 (10), 2609-2617. doi:10.5455/OVJ.2024.v14.i10.10


Turabian Style

Meisari, Suraida, Tita Damayanti Lestari, Djoko Legowo, Thomas Valentinus Widiyatno, Eka Pramyrtha Hestianah, Retno Sri Wahjuni, Ratna Damayanti, Aswin Rafif Khairullah, Ikechukwu Benjamin Moses, and Ricadonna Raissa. 2024. Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent. Open Veterinary Journal, 14 (10), 2609-2617. doi:10.5455/OVJ.2024.v14.i10.10


Chicago Style

Meisari, Suraida, Tita Damayanti Lestari, Djoko Legowo, Thomas Valentinus Widiyatno, Eka Pramyrtha Hestianah, Retno Sri Wahjuni, Ratna Damayanti, Aswin Rafif Khairullah, Ikechukwu Benjamin Moses, and Ricadonna Raissa. "Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent." Open Veterinary Journal 14 (2024), 2609-2617. doi:10.5455/OVJ.2024.v14.i10.10


MLA (The Modern Language Association) Style

Meisari, Suraida, Tita Damayanti Lestari, Djoko Legowo, Thomas Valentinus Widiyatno, Eka Pramyrtha Hestianah, Retno Sri Wahjuni, Ratna Damayanti, Aswin Rafif Khairullah, Ikechukwu Benjamin Moses, and Ricadonna Raissa. "Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent." Open Veterinary Journal 14.10 (2024), 2609-2617. Print. doi:10.5455/OVJ.2024.v14.i10.10


APA (American Psychological Association) Style

Meisari, S., Lestari, . T. D., Legowo, . D., Widiyatno, . T. V., Hestianah, . E. P., Wahjuni, . R. S., Damayanti, . R., Khairullah, . A. R., Moses, . I. B. & Raissa, . R. (2024) Identification of different blood concentration of domestic cat (Felis catus) with Leucomalachite Green and Takayama reagent. Open Veterinary Journal, 14 (10), 2609-2617. doi:10.5455/OVJ.2024.v14.i10.10


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly