E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 12
14 / 12Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Research Article


Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool

Jihen Taleb, Saida Ncibi, Sabah Dhibi, Khaled Athmouni, Najet Ghouaidia, Aida Dhibi, Abdurraouf Zaet, Lazhar Zourgui.


Abstract
Background:
Several studies reported the health benefits of Opuntia ficus indica extracts.

Aim:
This study aimed to (1) optimizes extraction conditions. (2) Point out its antioxidant and cytoprotective potential against cadmium toxicity in rat liver homogenate cells and HepG2.

Methods:
This experiment used the response surface methodology. It helped to determine the optimum conditions necessary to get the highest phenolic compound yield from Opuntia ficus indica cladodes aqueous extract. Then, a chromatography analysis was conducted to determine the phenolic compounds present in this extract. To explore the antioxidant abilities of this extract, this study used superoxide radical and hydroxyl radicals scavenging assays. Finally, to explore its biological benefits and its protective effect against cadmium toxicity, these research investigators carried out (1) an in vitro protein glycation test (2) MTT and inverted microscope imaging in HepG2 and HEK293 cell line (3) a lipid peroxidation test using rat erythrocytes.

Results:
The best extraction conditions were 10 minutes for the extraction time, 80°C for the temperature, and 30 ml/g for the liquid-solid ratio. The in vitro tests showed that Opuntia ficus indica aqueous extract has a high capacity to disable the free radical effect and prevent HepG2 and erythrocytes. Besides, the HPLC method identified 5 phenolic compounds within this extract.

Conclusion:
This study confirmed the health benefits of Opuntia ficus indica. Thus, more attention should be given to investigating this plant's capacities and isolating and identifying its bioactive molecules.

Key words: Antioxidant, Cytoprotective, Extraction, Optimization, Opuntia ficus indica cladode


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Jihen Taleb
Articles by Saida Ncibi
Articles by Sabah Dhibi
Articles by Khaled Athmouni
Articles by Najet Ghouaidia
Articles by Aida Dhibi
Articles by Abdurraouf Zaet
Articles by Lazhar Zourgui
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Taleb J, Ncibi S, Dhibi S, Athmouni K, Ghouaidia N, Dhibi A, Zaet A, Zourgui L. Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool. Open Vet J. 2024; 14(12): 3257-3268. doi:10.5455/OVJ.2024.v14.i12.11

Web Style

Taleb J, Ncibi S, Dhibi S, Athmouni K, Ghouaidia N, Dhibi A, Zaet A, Zourgui L. Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool. https://www.openveterinaryjournal.com/?mno=215882 [Access: January 22, 2025]. doi:10.5455/OVJ.2024.v14.i12.11

AMA (American Medical Association) Style

Taleb J, Ncibi S, Dhibi S, Athmouni K, Ghouaidia N, Dhibi A, Zaet A, Zourgui L. Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool. Open Vet J. 2024; 14(12): 3257-3268. doi:10.5455/OVJ.2024.v14.i12.11


Vancouver/ICMJE Style

Taleb J, Ncibi S, Dhibi S, Athmouni K, Ghouaidia N, Dhibi A, Zaet A, Zourgui L. Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool. Open Vet J. (2024), [cited January 22, 2025]; 14(12): 3257-3268. doi:10.5455/OVJ.2024.v14.i12.11


Harvard Style

Taleb, J., Ncibi, . S., Dhibi, . S., Athmouni, . K., Ghouaidia, . N., Dhibi, . A., Zaet, . A. & Zourgui, . L. (2024) Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool. Open Vet J, 14 (12), 3257-3268. doi:10.5455/OVJ.2024.v14.i12.11


Turabian Style

Taleb, Jihen, Saida Ncibi, Sabah Dhibi, Khaled Athmouni, Najet Ghouaidia, Aida Dhibi, Abdurraouf Zaet, and Lazhar Zourgui. 2024. Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool. Open Veterinary Journal, 14 (12), 3257-3268. doi:10.5455/OVJ.2024.v14.i12.11


Chicago Style

Taleb, Jihen, Saida Ncibi, Sabah Dhibi, Khaled Athmouni, Najet Ghouaidia, Aida Dhibi, Abdurraouf Zaet, and Lazhar Zourgui. "Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool." Open Veterinary Journal 14 (2024), 3257-3268. doi:10.5455/OVJ.2024.v14.i12.11


MLA (The Modern Language Association) Style

Taleb, Jihen, Saida Ncibi, Sabah Dhibi, Khaled Athmouni, Najet Ghouaidia, Aida Dhibi, Abdurraouf Zaet, and Lazhar Zourgui. "Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool." Open Veterinary Journal 14.12 (2024), 3257-3268. Print. doi:10.5455/OVJ.2024.v14.i12.11


APA (American Psychological Association) Style

Taleb, J., Ncibi, . S., Dhibi, . S., Athmouni, . K., Ghouaidia, . N., Dhibi, . A., Zaet, . A. & Zourgui, . L. (2024) Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool. Open Veterinary Journal, 14 (12), 3257-3268. doi:10.5455/OVJ.2024.v14.i12.11


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly