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Research Article


Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin

Melpa Susanti Purba, Dito Anggoro, Naohiro Yamamoto, Sota Yoshimine, Junichi Murakami, Toshiki Tanaka, Kimikazu Hamano, Harumichi Itoh, Kazuhito Itamoto, Yuki Nemoto, Munekazu Nakaichi, Hiroshi Sunahara, Kenji Tani.


Abstract
Background:
Cell sheet therapy has been developed as an effective regenerative medicine to improve wound repair treatment and prevent postoperative complications. Fibroblasts are widely used to create cell sheet engineering because of their essential role in tissue regeneration and the acceleration of the wound healing process.

Aim:
The study aimed to establish the multilayered fibroblast sheets from canine fibroblast.

Methods:
The study used fibroblasts from three origin canine tissues, including oral mucosa, skin, and tail skin. A canine fibroblast sheet was produced using the large-numbers cell seeding method with Rho kinase inhibitor. The assessment included viability cells, histological evaluation of the sheet, and secretion of vascular endothelial growth factor (VEGF) and monocyte chemoattractant protein-1 (MCP-1) of the fibroblast sheet.

Results:
The three fibroblast groups successfully established a multilayered fibroblast sheet. Hematoxylin and eosin staining examination showed the thickest sheet in the oral mucosa fibroblast sheet. Immunohistochemical examination revealed that the multilayered cell sheet comprised fibroblasts expressed by vimentin. All multilayered fibroblast sheet groups secreted the VEGF and MCP-1. No differences were observed in the viability cells of each origin cell.

Conclusion:
The multilayered fibroblast sheets were successfully established from canine fibroblast. This finding provides the primary data to develop regenerative medicine in the veterinary field.

Key words: Angiogenesis, Canine, Regenerative medicine, Rho kinase inhibitor


 
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How to Cite this Article
Pubmed Style

Purba MS, Anggoro D, Yamamoto N, Yoshimine S, Murakami J, Tanaka T, Hamano K, Itoh H, Itamoto K, Nemoto Y, Nakaichi M, Sunahara H, Tani K. Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin. Open Vet J. 2024; 14(12): 3428-3439. doi:10.5455/OVJ.2024.v14.i12.27

Web Style

Purba MS, Anggoro D, Yamamoto N, Yoshimine S, Murakami J, Tanaka T, Hamano K, Itoh H, Itamoto K, Nemoto Y, Nakaichi M, Sunahara H, Tani K. Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin. https://www.openveterinaryjournal.com/?mno=222546 [Access: January 21, 2025]. doi:10.5455/OVJ.2024.v14.i12.27

AMA (American Medical Association) Style

Purba MS, Anggoro D, Yamamoto N, Yoshimine S, Murakami J, Tanaka T, Hamano K, Itoh H, Itamoto K, Nemoto Y, Nakaichi M, Sunahara H, Tani K. Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin. Open Vet J. 2024; 14(12): 3428-3439. doi:10.5455/OVJ.2024.v14.i12.27


Vancouver/ICMJE Style

Purba MS, Anggoro D, Yamamoto N, Yoshimine S, Murakami J, Tanaka T, Hamano K, Itoh H, Itamoto K, Nemoto Y, Nakaichi M, Sunahara H, Tani K. Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin. Open Vet J. (2024), [cited January 21, 2025]; 14(12): 3428-3439. doi:10.5455/OVJ.2024.v14.i12.27


Harvard Style

Purba, M. S., Anggoro, . D., Yamamoto, . N., Yoshimine, . S., Murakami, . J., Tanaka, . T., Hamano, . K., Itoh, . H., Itamoto, . K., Nemoto, . Y., Nakaichi, . M., Sunahara, . H. & Tani, . K. (2024) Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin. Open Vet J, 14 (12), 3428-3439. doi:10.5455/OVJ.2024.v14.i12.27


Turabian Style

Purba, Melpa Susanti, Dito Anggoro, Naohiro Yamamoto, Sota Yoshimine, Junichi Murakami, Toshiki Tanaka, Kimikazu Hamano, Harumichi Itoh, Kazuhito Itamoto, Yuki Nemoto, Munekazu Nakaichi, Hiroshi Sunahara, and Kenji Tani. 2024. Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin. Open Veterinary Journal, 14 (12), 3428-3439. doi:10.5455/OVJ.2024.v14.i12.27


Chicago Style

Purba, Melpa Susanti, Dito Anggoro, Naohiro Yamamoto, Sota Yoshimine, Junichi Murakami, Toshiki Tanaka, Kimikazu Hamano, Harumichi Itoh, Kazuhito Itamoto, Yuki Nemoto, Munekazu Nakaichi, Hiroshi Sunahara, and Kenji Tani. "Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin." Open Veterinary Journal 14 (2024), 3428-3439. doi:10.5455/OVJ.2024.v14.i12.27


MLA (The Modern Language Association) Style

Purba, Melpa Susanti, Dito Anggoro, Naohiro Yamamoto, Sota Yoshimine, Junichi Murakami, Toshiki Tanaka, Kimikazu Hamano, Harumichi Itoh, Kazuhito Itamoto, Yuki Nemoto, Munekazu Nakaichi, Hiroshi Sunahara, and Kenji Tani. "Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin." Open Veterinary Journal 14.12 (2024), 3428-3439. Print. doi:10.5455/OVJ.2024.v14.i12.27


APA (American Psychological Association) Style

Purba, M. S., Anggoro, . D., Yamamoto, . N., Yoshimine, . S., Murakami, . J., Tanaka, . T., Hamano, . K., Itoh, . H., Itamoto, . K., Nemoto, . Y., Nakaichi, . M., Sunahara, . H. & Tani, . K. (2024) Establishment and characterization of multilayered fibroblast cell sheets from the canine oral mucosa, skin, and tail skin. Open Veterinary Journal, 14 (12), 3428-3439. doi:10.5455/OVJ.2024.v14.i12.27


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